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A Novel Function of TET2 in CNS: Sustaining Neuronal Survival
DNA dioxygenases Ten-Eleven Translocation (TET) proteins can catalyze the conversion of 5-methylcytosine (5mC) of DNA to 5-hydroxymethylcytosine (5hmC), and thereby alter the epigenetic state of DNA. The TET family includes TET1, TET2 and TET3 members in mammals. Recently, accumulative research unco...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613284/ https://www.ncbi.nlm.nih.gov/pubmed/26378518 http://dx.doi.org/10.3390/ijms160921846 |
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author | Mi, Yajing Gao, Xingchun Dai, Jinxiang Ma, Yue Xu, Lixian Jin, Weilin |
author_facet | Mi, Yajing Gao, Xingchun Dai, Jinxiang Ma, Yue Xu, Lixian Jin, Weilin |
author_sort | Mi, Yajing |
collection | PubMed |
description | DNA dioxygenases Ten-Eleven Translocation (TET) proteins can catalyze the conversion of 5-methylcytosine (5mC) of DNA to 5-hydroxymethylcytosine (5hmC), and thereby alter the epigenetic state of DNA. The TET family includes TET1, TET2 and TET3 members in mammals. Recently, accumulative research uncovered that TET1–3 occur abundantly in the central nervous system (CNS), and their biological functions have just begun to be investigated. In the present study, we demonstrated that mRNA and protein of TET2 were highly expressed in the cerebral cortex and hippocampus along the whole brain-development process. Further studies showed that TET2 was expressed in various types of cells, especially in most neurons. Subcellular distribution pattern implicated that TET2 is localized in both nucleus and cytoplasm of neurons. Down-regulation of TET2 in cultured cortical neurons with RNA interference implied that TET2 was required for cell survival. In all, our results indicate that neuronal TET2 is positively involved in the regulation of cell survival. |
format | Online Article Text |
id | pubmed-4613284 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-46132842015-10-26 A Novel Function of TET2 in CNS: Sustaining Neuronal Survival Mi, Yajing Gao, Xingchun Dai, Jinxiang Ma, Yue Xu, Lixian Jin, Weilin Int J Mol Sci Article DNA dioxygenases Ten-Eleven Translocation (TET) proteins can catalyze the conversion of 5-methylcytosine (5mC) of DNA to 5-hydroxymethylcytosine (5hmC), and thereby alter the epigenetic state of DNA. The TET family includes TET1, TET2 and TET3 members in mammals. Recently, accumulative research uncovered that TET1–3 occur abundantly in the central nervous system (CNS), and their biological functions have just begun to be investigated. In the present study, we demonstrated that mRNA and protein of TET2 were highly expressed in the cerebral cortex and hippocampus along the whole brain-development process. Further studies showed that TET2 was expressed in various types of cells, especially in most neurons. Subcellular distribution pattern implicated that TET2 is localized in both nucleus and cytoplasm of neurons. Down-regulation of TET2 in cultured cortical neurons with RNA interference implied that TET2 was required for cell survival. In all, our results indicate that neuronal TET2 is positively involved in the regulation of cell survival. MDPI 2015-09-10 /pmc/articles/PMC4613284/ /pubmed/26378518 http://dx.doi.org/10.3390/ijms160921846 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mi, Yajing Gao, Xingchun Dai, Jinxiang Ma, Yue Xu, Lixian Jin, Weilin A Novel Function of TET2 in CNS: Sustaining Neuronal Survival |
title | A Novel Function of TET2 in CNS: Sustaining Neuronal Survival |
title_full | A Novel Function of TET2 in CNS: Sustaining Neuronal Survival |
title_fullStr | A Novel Function of TET2 in CNS: Sustaining Neuronal Survival |
title_full_unstemmed | A Novel Function of TET2 in CNS: Sustaining Neuronal Survival |
title_short | A Novel Function of TET2 in CNS: Sustaining Neuronal Survival |
title_sort | novel function of tet2 in cns: sustaining neuronal survival |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613284/ https://www.ncbi.nlm.nih.gov/pubmed/26378518 http://dx.doi.org/10.3390/ijms160921846 |
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