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Metabolite Profiling of Diverse Rice Germplasm and Identification of Conserved Metabolic Markers of Rice Roots in Response to Long-Term Mild Salinity Stress

The sensitivity of rice to salt stress greatly depends on growth stages, organ types and cultivars. Especially, the roots of young rice seedlings are highly salt-sensitive organs that limit plant growth, even under mild soil salinity conditions. In an attempt to identify metabolic markers of rice ro...

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Autores principales: Nam, Myung Hee, Bang, Eunjung, Kwon, Taek Yun, Kim, Yuran, Kim, Eun Hee, Cho, Kyungwon, Park, Woong June, Kim, Beom-Gi, Yoon, In Sun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613291/
https://www.ncbi.nlm.nih.gov/pubmed/26378525
http://dx.doi.org/10.3390/ijms160921959
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author Nam, Myung Hee
Bang, Eunjung
Kwon, Taek Yun
Kim, Yuran
Kim, Eun Hee
Cho, Kyungwon
Park, Woong June
Kim, Beom-Gi
Yoon, In Sun
author_facet Nam, Myung Hee
Bang, Eunjung
Kwon, Taek Yun
Kim, Yuran
Kim, Eun Hee
Cho, Kyungwon
Park, Woong June
Kim, Beom-Gi
Yoon, In Sun
author_sort Nam, Myung Hee
collection PubMed
description The sensitivity of rice to salt stress greatly depends on growth stages, organ types and cultivars. Especially, the roots of young rice seedlings are highly salt-sensitive organs that limit plant growth, even under mild soil salinity conditions. In an attempt to identify metabolic markers of rice roots responding to salt stress, metabolite profiling was performed by (1)H-NMR spectroscopy in 38 rice genotypes that varied in biomass accumulation under long-term mild salinity condition. Multivariate statistical analysis showed separation of the control and salt-treated rice roots and rice genotypes with differential growth potential. By quantitative analyses of (1)H-NMR data, five conserved salt-responsive metabolic markers of rice roots were identified. Sucrose, allantoin and glutamate accumulated by salt stress, whereas the levels of glutamine and alanine decreased. A positive correlation of metabolite changes with growth potential and salt tolerance of rice genotypes was observed for allantoin and glutamine. Adjustment of nitrogen metabolism in rice roots is likely to be closely related to maintain the growth potential and increase the stress tolerance of rice.
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spelling pubmed-46132912015-10-26 Metabolite Profiling of Diverse Rice Germplasm and Identification of Conserved Metabolic Markers of Rice Roots in Response to Long-Term Mild Salinity Stress Nam, Myung Hee Bang, Eunjung Kwon, Taek Yun Kim, Yuran Kim, Eun Hee Cho, Kyungwon Park, Woong June Kim, Beom-Gi Yoon, In Sun Int J Mol Sci Article The sensitivity of rice to salt stress greatly depends on growth stages, organ types and cultivars. Especially, the roots of young rice seedlings are highly salt-sensitive organs that limit plant growth, even under mild soil salinity conditions. In an attempt to identify metabolic markers of rice roots responding to salt stress, metabolite profiling was performed by (1)H-NMR spectroscopy in 38 rice genotypes that varied in biomass accumulation under long-term mild salinity condition. Multivariate statistical analysis showed separation of the control and salt-treated rice roots and rice genotypes with differential growth potential. By quantitative analyses of (1)H-NMR data, five conserved salt-responsive metabolic markers of rice roots were identified. Sucrose, allantoin and glutamate accumulated by salt stress, whereas the levels of glutamine and alanine decreased. A positive correlation of metabolite changes with growth potential and salt tolerance of rice genotypes was observed for allantoin and glutamine. Adjustment of nitrogen metabolism in rice roots is likely to be closely related to maintain the growth potential and increase the stress tolerance of rice. MDPI 2015-09-11 /pmc/articles/PMC4613291/ /pubmed/26378525 http://dx.doi.org/10.3390/ijms160921959 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nam, Myung Hee
Bang, Eunjung
Kwon, Taek Yun
Kim, Yuran
Kim, Eun Hee
Cho, Kyungwon
Park, Woong June
Kim, Beom-Gi
Yoon, In Sun
Metabolite Profiling of Diverse Rice Germplasm and Identification of Conserved Metabolic Markers of Rice Roots in Response to Long-Term Mild Salinity Stress
title Metabolite Profiling of Diverse Rice Germplasm and Identification of Conserved Metabolic Markers of Rice Roots in Response to Long-Term Mild Salinity Stress
title_full Metabolite Profiling of Diverse Rice Germplasm and Identification of Conserved Metabolic Markers of Rice Roots in Response to Long-Term Mild Salinity Stress
title_fullStr Metabolite Profiling of Diverse Rice Germplasm and Identification of Conserved Metabolic Markers of Rice Roots in Response to Long-Term Mild Salinity Stress
title_full_unstemmed Metabolite Profiling of Diverse Rice Germplasm and Identification of Conserved Metabolic Markers of Rice Roots in Response to Long-Term Mild Salinity Stress
title_short Metabolite Profiling of Diverse Rice Germplasm and Identification of Conserved Metabolic Markers of Rice Roots in Response to Long-Term Mild Salinity Stress
title_sort metabolite profiling of diverse rice germplasm and identification of conserved metabolic markers of rice roots in response to long-term mild salinity stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613291/
https://www.ncbi.nlm.nih.gov/pubmed/26378525
http://dx.doi.org/10.3390/ijms160921959
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