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Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae)

The autumnal moth (Epirrita autumnata) is a cyclically outbreaking forest Lepidoptera with circumpolar distribution and substantial impact on Northern ecosystems. We have isolated 21 microsatellites from the species to facilitate population genetic studies of population cycles, outbreaks, and crashe...

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Autores principales: Aarnes, Siv Grethe, Fløystad, Ida, Schregel, Julia, Vindstad, Ole Petter Laksforsmo, Jepsen, Jane Uhd, Eiken, Hans Geir, Ims, Rolf A., Hagen, Snorre B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613323/
https://www.ncbi.nlm.nih.gov/pubmed/26393576
http://dx.doi.org/10.3390/ijms160922541
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author Aarnes, Siv Grethe
Fløystad, Ida
Schregel, Julia
Vindstad, Ole Petter Laksforsmo
Jepsen, Jane Uhd
Eiken, Hans Geir
Ims, Rolf A.
Hagen, Snorre B.
author_facet Aarnes, Siv Grethe
Fløystad, Ida
Schregel, Julia
Vindstad, Ole Petter Laksforsmo
Jepsen, Jane Uhd
Eiken, Hans Geir
Ims, Rolf A.
Hagen, Snorre B.
author_sort Aarnes, Siv Grethe
collection PubMed
description The autumnal moth (Epirrita autumnata) is a cyclically outbreaking forest Lepidoptera with circumpolar distribution and substantial impact on Northern ecosystems. We have isolated 21 microsatellites from the species to facilitate population genetic studies of population cycles, outbreaks, and crashes. First, PCR primers and PCR conditions were developed to amplify 19 trinucleotide loci and two tetranucleotide loci in six multiplex PCR approaches and then analyzed for species specificity, sensitivity and precision. Twelve of the loci showed simple tandem repeat array structures while nine loci showed imperfect repeat structures, and repeat numbers varied in our material between six and 15. The application in population genetics for all the 21 microsatellites were further validated in 48 autumnal moths sampled from Northern Norway, and allelic variation was detected in 19 loci. The detected numbers of alleles per locus ranged from two to 13, and the observed and expected heterozygosities varied from 0.04 to 0.69 and 0.04 to 0.79, respectively. Evidence for linkage disequilibrium was found for six loci as well as indication of one null allele. We find that these novel microsatellites and their multiplex-PCR assays are suitable for further research on fine- and large-scale population-genetic studies of Epirrita autumnata.
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spelling pubmed-46133232015-10-26 Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae) Aarnes, Siv Grethe Fløystad, Ida Schregel, Julia Vindstad, Ole Petter Laksforsmo Jepsen, Jane Uhd Eiken, Hans Geir Ims, Rolf A. Hagen, Snorre B. Int J Mol Sci Article The autumnal moth (Epirrita autumnata) is a cyclically outbreaking forest Lepidoptera with circumpolar distribution and substantial impact on Northern ecosystems. We have isolated 21 microsatellites from the species to facilitate population genetic studies of population cycles, outbreaks, and crashes. First, PCR primers and PCR conditions were developed to amplify 19 trinucleotide loci and two tetranucleotide loci in six multiplex PCR approaches and then analyzed for species specificity, sensitivity and precision. Twelve of the loci showed simple tandem repeat array structures while nine loci showed imperfect repeat structures, and repeat numbers varied in our material between six and 15. The application in population genetics for all the 21 microsatellites were further validated in 48 autumnal moths sampled from Northern Norway, and allelic variation was detected in 19 loci. The detected numbers of alleles per locus ranged from two to 13, and the observed and expected heterozygosities varied from 0.04 to 0.69 and 0.04 to 0.79, respectively. Evidence for linkage disequilibrium was found for six loci as well as indication of one null allele. We find that these novel microsatellites and their multiplex-PCR assays are suitable for further research on fine- and large-scale population-genetic studies of Epirrita autumnata. MDPI 2015-09-17 /pmc/articles/PMC4613323/ /pubmed/26393576 http://dx.doi.org/10.3390/ijms160922541 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aarnes, Siv Grethe
Fløystad, Ida
Schregel, Julia
Vindstad, Ole Petter Laksforsmo
Jepsen, Jane Uhd
Eiken, Hans Geir
Ims, Rolf A.
Hagen, Snorre B.
Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae)
title Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae)
title_full Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae)
title_fullStr Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae)
title_full_unstemmed Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae)
title_short Identification and Evaluation of 21 Novel Microsatellite Markers from the Autumnal Moth (Epirrita autumnata) (Lepidoptera: Geometridae)
title_sort identification and evaluation of 21 novel microsatellite markers from the autumnal moth (epirrita autumnata) (lepidoptera: geometridae)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613323/
https://www.ncbi.nlm.nih.gov/pubmed/26393576
http://dx.doi.org/10.3390/ijms160922541
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