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Role of miRNA-146 in proliferation and differentiation of mouse neural stem cells
Neural stem cells (NSCs) have been defined as neural cells with the potential to self-renew and eventually generate all cell types of the nervous system. NSCs serve as an ideal cell type for nervous system repair. In the present study, miR-146 overexpression and predicted target (notch 1) were used...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613701/ https://www.ncbi.nlm.nih.gov/pubmed/26199426 http://dx.doi.org/10.1042/BSR20150088 |
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author | Xiao, Wei-Zhong Lu, An-Qing Liu, Xin-Wei Li, Zhe Zi, Ying Wang, Zhi-Wei |
author_facet | Xiao, Wei-Zhong Lu, An-Qing Liu, Xin-Wei Li, Zhe Zi, Ying Wang, Zhi-Wei |
author_sort | Xiao, Wei-Zhong |
collection | PubMed |
description | Neural stem cells (NSCs) have been defined as neural cells with the potential to self-renew and eventually generate all cell types of the nervous system. NSCs serve as an ideal cell type for nervous system repair. In the present study, miR-146 overexpression and predicted target (notch 1) were used to study proliferation and differentiation of mouse NSCs. shRNA were used to demonstrate the function of Notch 1 in proliferation of mouse NSCs and luciferase reporter assay was used to assess and confirm the binding sequence of 3′-UTR between Notch 1 and miR-146. Results showed that miR-146 overexpression and knockdown of notch 1 inhibited proliferation of mouse NSCs under serum-free cultural conditions and promoted spontaneous differentiation of mouse NSCs under contained serum cultural conditions respectively. Mouse NSCs spontaneously underwent differentiation into neurogenic cells with contained serum medium. However, when miR-146 was overexpressed, differentiation efficiency of glial cells from NSCs was increased, suggesting that Notch1 promoted NSC proliferation and repressed spontaneous differentiation of NSC in serum-free medium. In conclusion, our results demonstrate that miR-146 promoted spontaneous differentiation of NSCs, and this mechanism was influenced by miR-146, as well as its target (notch 1) and downstream gene. |
format | Online Article Text |
id | pubmed-4613701 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-46137012015-11-02 Role of miRNA-146 in proliferation and differentiation of mouse neural stem cells Xiao, Wei-Zhong Lu, An-Qing Liu, Xin-Wei Li, Zhe Zi, Ying Wang, Zhi-Wei Biosci Rep Original Papers Neural stem cells (NSCs) have been defined as neural cells with the potential to self-renew and eventually generate all cell types of the nervous system. NSCs serve as an ideal cell type for nervous system repair. In the present study, miR-146 overexpression and predicted target (notch 1) were used to study proliferation and differentiation of mouse NSCs. shRNA were used to demonstrate the function of Notch 1 in proliferation of mouse NSCs and luciferase reporter assay was used to assess and confirm the binding sequence of 3′-UTR between Notch 1 and miR-146. Results showed that miR-146 overexpression and knockdown of notch 1 inhibited proliferation of mouse NSCs under serum-free cultural conditions and promoted spontaneous differentiation of mouse NSCs under contained serum cultural conditions respectively. Mouse NSCs spontaneously underwent differentiation into neurogenic cells with contained serum medium. However, when miR-146 was overexpressed, differentiation efficiency of glial cells from NSCs was increased, suggesting that Notch1 promoted NSC proliferation and repressed spontaneous differentiation of NSC in serum-free medium. In conclusion, our results demonstrate that miR-146 promoted spontaneous differentiation of NSCs, and this mechanism was influenced by miR-146, as well as its target (notch 1) and downstream gene. Portland Press Ltd. 2015-09-10 /pmc/articles/PMC4613701/ /pubmed/26199426 http://dx.doi.org/10.1042/BSR20150088 Text en © 2015 Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article published by Portland Press Limited and distributed under the Creative Commons Attribution License 3.0. (http://creativecommons.org/licenses/by/3.0/) |
spellingShingle | Original Papers Xiao, Wei-Zhong Lu, An-Qing Liu, Xin-Wei Li, Zhe Zi, Ying Wang, Zhi-Wei Role of miRNA-146 in proliferation and differentiation of mouse neural stem cells |
title | Role of miRNA-146 in proliferation and differentiation of mouse neural stem cells |
title_full | Role of miRNA-146 in proliferation and differentiation of mouse neural stem cells |
title_fullStr | Role of miRNA-146 in proliferation and differentiation of mouse neural stem cells |
title_full_unstemmed | Role of miRNA-146 in proliferation and differentiation of mouse neural stem cells |
title_short | Role of miRNA-146 in proliferation and differentiation of mouse neural stem cells |
title_sort | role of mirna-146 in proliferation and differentiation of mouse neural stem cells |
topic | Original Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613701/ https://www.ncbi.nlm.nih.gov/pubmed/26199426 http://dx.doi.org/10.1042/BSR20150088 |
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