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Adenylylsulfate–ammonia adenylyltransferase activity is another inherent property of Fhit proteins
Fhits (fragile histidine triad proteins) occur in eukaryotes but their function is largely unknown, although human Fhit is believed to act as a tumour suppressor. Fhits also exhibit dinucleoside triphosphatase, adenylylsulfatase and nucleoside phosphoramidase activities that in each case yield nucle...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613722/ https://www.ncbi.nlm.nih.gov/pubmed/26181368 http://dx.doi.org/10.1042/BSR20150135 |
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author | Wojdyła-Mamoń, Anna M. Guranowski, Andrzej |
author_facet | Wojdyła-Mamoń, Anna M. Guranowski, Andrzej |
author_sort | Wojdyła-Mamoń, Anna M. |
collection | PubMed |
description | Fhits (fragile histidine triad proteins) occur in eukaryotes but their function is largely unknown, although human Fhit is believed to act as a tumour suppressor. Fhits also exhibit dinucleoside triphosphatase, adenylylsulfatase and nucleoside phosphoramidase activities that in each case yield nucleoside 5′-monophosphate as a product. Due to the dinucleoside triphosphatase activity, Fhits may also be involved in mRNA decapping. In the present study, we demonstrate Fhit-catalysed ammonolysis of adenosine 5′-phosphosulfate, which results in the formation of adenosine 5′-phosphoramidate. This reaction has previously been associated with adenylylsulfate–ammonia adenylyltransferase (EC 2.7.7.51). Our finding shows that the capacity to catalyse ammonolysis is another inherent property of Fhits. Basic kinetic parameters and substrate specificity of this reaction catalysed by human Fhit are presented. |
format | Online Article Text |
id | pubmed-4613722 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-46137222015-11-02 Adenylylsulfate–ammonia adenylyltransferase activity is another inherent property of Fhit proteins Wojdyła-Mamoń, Anna M. Guranowski, Andrzej Biosci Rep Original Papers Fhits (fragile histidine triad proteins) occur in eukaryotes but their function is largely unknown, although human Fhit is believed to act as a tumour suppressor. Fhits also exhibit dinucleoside triphosphatase, adenylylsulfatase and nucleoside phosphoramidase activities that in each case yield nucleoside 5′-monophosphate as a product. Due to the dinucleoside triphosphatase activity, Fhits may also be involved in mRNA decapping. In the present study, we demonstrate Fhit-catalysed ammonolysis of adenosine 5′-phosphosulfate, which results in the formation of adenosine 5′-phosphoramidate. This reaction has previously been associated with adenylylsulfate–ammonia adenylyltransferase (EC 2.7.7.51). Our finding shows that the capacity to catalyse ammonolysis is another inherent property of Fhits. Basic kinetic parameters and substrate specificity of this reaction catalysed by human Fhit are presented. Portland Press Ltd. 2015-08-07 /pmc/articles/PMC4613722/ /pubmed/26181368 http://dx.doi.org/10.1042/BSR20150135 Text en © 2015 Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article published by Portland Press Limited and distributed under the Creative Commons Attribution Licence 3.0 (http://creativecommons.org/licenses/by/3.0/) . |
spellingShingle | Original Papers Wojdyła-Mamoń, Anna M. Guranowski, Andrzej Adenylylsulfate–ammonia adenylyltransferase activity is another inherent property of Fhit proteins |
title | Adenylylsulfate–ammonia adenylyltransferase activity is another inherent property of Fhit proteins |
title_full | Adenylylsulfate–ammonia adenylyltransferase activity is another inherent property of Fhit proteins |
title_fullStr | Adenylylsulfate–ammonia adenylyltransferase activity is another inherent property of Fhit proteins |
title_full_unstemmed | Adenylylsulfate–ammonia adenylyltransferase activity is another inherent property of Fhit proteins |
title_short | Adenylylsulfate–ammonia adenylyltransferase activity is another inherent property of Fhit proteins |
title_sort | adenylylsulfate–ammonia adenylyltransferase activity is another inherent property of fhit proteins |
topic | Original Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613722/ https://www.ncbi.nlm.nih.gov/pubmed/26181368 http://dx.doi.org/10.1042/BSR20150135 |
work_keys_str_mv | AT wojdyłamamonannam adenylylsulfateammoniaadenylyltransferaseactivityisanotherinherentpropertyoffhitproteins AT guranowskiandrzej adenylylsulfateammoniaadenylyltransferaseactivityisanotherinherentpropertyoffhitproteins |