Cargando…
Synaptic input changes to spinal cord motoneurons correlate with motor control impairments in a type 1 diabetes mellitus model
INTRODUCTION: Hyperglycemia is the main cause of diabetic complications, contributing to a widespread degeneration of the nervous system. Nevertheless, the main focus has been the sensory neurons because of neuropathic pain, while the impairments associated with the spinal cord and motor deficits, m...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4614060/ https://www.ncbi.nlm.nih.gov/pubmed/26516607 http://dx.doi.org/10.1002/brb3.372 |
_version_ | 1782396354608234496 |
---|---|
author | Benitez, Suzana Ulian Carneiro, Everardo Magalhães de Oliveira, Alexandre Leite Rodrigues |
author_facet | Benitez, Suzana Ulian Carneiro, Everardo Magalhães de Oliveira, Alexandre Leite Rodrigues |
author_sort | Benitez, Suzana Ulian |
collection | PubMed |
description | INTRODUCTION: Hyperglycemia is the main cause of diabetic complications, contributing to a widespread degeneration of the nervous system. Nevertheless, the main focus has been the sensory neurons because of neuropathic pain, while the impairments associated with the spinal cord and motor deficits, mostly of those initiated at early stages of the disease, have been poorly investigated. In this way, the present study used the nonobese diabetic mouse model to evaluate the microenvironment around motoneurons at ventral horn of the spinal cord, following prolonged hyperglycemia. METHODS: Adult female mice were divided into two groups: spontaneously diabetic (n = 33) and nondiabetic (n = 26). Mice were considered hyperglycemic when blood glucose surpassed 400 mg/dL. Following 2 weeks from that stage, part of the animals was euthanized and the lumbar intumescences were obtained and processed for immunohistochemistry and transmission electron microscopy. For immunohistochemistry, the antibodies used for integrated density of pixels quantification were anti‐synaptophysin, anti‐GFAP, and anti‐Iba1. The functional analysis was monitored with the walking track test (CatWalk system) during 4 weeks. RESULTS: The results revealed significant motor impairment in diabetic animals in comparison to the control group. Such loss of motor control correlated with a significant reduction in presynaptic terminals apposed to the motoneurons. Nevertheless, there were no significant changes in glial reaction in the spinal cord. CONCLUSION: Overall, the results herein revealed central nervous system changes at early stages of the disease that may in turn contribute to the motor deficit. Such changes open a new window of investigation in early stages of diabetes to better comprehend motor impairment as a long‐term complication of the disease. |
format | Online Article Text |
id | pubmed-4614060 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-46140602015-10-29 Synaptic input changes to spinal cord motoneurons correlate with motor control impairments in a type 1 diabetes mellitus model Benitez, Suzana Ulian Carneiro, Everardo Magalhães de Oliveira, Alexandre Leite Rodrigues Brain Behav Original Research INTRODUCTION: Hyperglycemia is the main cause of diabetic complications, contributing to a widespread degeneration of the nervous system. Nevertheless, the main focus has been the sensory neurons because of neuropathic pain, while the impairments associated with the spinal cord and motor deficits, mostly of those initiated at early stages of the disease, have been poorly investigated. In this way, the present study used the nonobese diabetic mouse model to evaluate the microenvironment around motoneurons at ventral horn of the spinal cord, following prolonged hyperglycemia. METHODS: Adult female mice were divided into two groups: spontaneously diabetic (n = 33) and nondiabetic (n = 26). Mice were considered hyperglycemic when blood glucose surpassed 400 mg/dL. Following 2 weeks from that stage, part of the animals was euthanized and the lumbar intumescences were obtained and processed for immunohistochemistry and transmission electron microscopy. For immunohistochemistry, the antibodies used for integrated density of pixels quantification were anti‐synaptophysin, anti‐GFAP, and anti‐Iba1. The functional analysis was monitored with the walking track test (CatWalk system) during 4 weeks. RESULTS: The results revealed significant motor impairment in diabetic animals in comparison to the control group. Such loss of motor control correlated with a significant reduction in presynaptic terminals apposed to the motoneurons. Nevertheless, there were no significant changes in glial reaction in the spinal cord. CONCLUSION: Overall, the results herein revealed central nervous system changes at early stages of the disease that may in turn contribute to the motor deficit. Such changes open a new window of investigation in early stages of diabetes to better comprehend motor impairment as a long‐term complication of the disease. John Wiley and Sons Inc. 2015-09-09 /pmc/articles/PMC4614060/ /pubmed/26516607 http://dx.doi.org/10.1002/brb3.372 Text en © 2015 The Authors. Brain and Behavior published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Benitez, Suzana Ulian Carneiro, Everardo Magalhães de Oliveira, Alexandre Leite Rodrigues Synaptic input changes to spinal cord motoneurons correlate with motor control impairments in a type 1 diabetes mellitus model |
title | Synaptic input changes to spinal cord motoneurons correlate with motor control impairments in a type 1 diabetes mellitus model |
title_full | Synaptic input changes to spinal cord motoneurons correlate with motor control impairments in a type 1 diabetes mellitus model |
title_fullStr | Synaptic input changes to spinal cord motoneurons correlate with motor control impairments in a type 1 diabetes mellitus model |
title_full_unstemmed | Synaptic input changes to spinal cord motoneurons correlate with motor control impairments in a type 1 diabetes mellitus model |
title_short | Synaptic input changes to spinal cord motoneurons correlate with motor control impairments in a type 1 diabetes mellitus model |
title_sort | synaptic input changes to spinal cord motoneurons correlate with motor control impairments in a type 1 diabetes mellitus model |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4614060/ https://www.ncbi.nlm.nih.gov/pubmed/26516607 http://dx.doi.org/10.1002/brb3.372 |
work_keys_str_mv | AT benitezsuzanaulian synapticinputchangestospinalcordmotoneuronscorrelatewithmotorcontrolimpairmentsinatype1diabetesmellitusmodel AT carneiroeverardomagalhaes synapticinputchangestospinalcordmotoneuronscorrelatewithmotorcontrolimpairmentsinatype1diabetesmellitusmodel AT deoliveiraalexandreleiterodrigues synapticinputchangestospinalcordmotoneuronscorrelatewithmotorcontrolimpairmentsinatype1diabetesmellitusmodel |