A single design strategy for dual sensitive pH probe with a suitable range to map pH in living cells

Due to the lack of a proper imaging approach, a veracious pH map of normal and abnormal cell is still rare. In this work, we presented a rhodamine-salicylaldehyde combination (Rh-SA2) as a novel pH probe, which has dual sensitive units for both acidic and basic environment. This dual sensitive probe...

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Autores principales: Yu, Kang-Kang, Hou, Ji-Ting, Li, Kun, Yao, Qian, Yang, Jin, Wu, Ming-Yu, Xie, Yong-Mei, Yu, Xiao-Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4614390/
https://www.ncbi.nlm.nih.gov/pubmed/26486180
http://dx.doi.org/10.1038/srep15540
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author Yu, Kang-Kang
Hou, Ji-Ting
Li, Kun
Yao, Qian
Yang, Jin
Wu, Ming-Yu
Xie, Yong-Mei
Yu, Xiao-Qi
author_facet Yu, Kang-Kang
Hou, Ji-Ting
Li, Kun
Yao, Qian
Yang, Jin
Wu, Ming-Yu
Xie, Yong-Mei
Yu, Xiao-Qi
author_sort Yu, Kang-Kang
collection PubMed
description Due to the lack of a proper imaging approach, a veracious pH map of normal and abnormal cell is still rare. In this work, we presented a rhodamine-salicylaldehyde combination (Rh-SA2) as a novel pH probe, which has dual sensitive units for both acidic and basic environment. This dual sensitive probe acts like a chameleon in living cells and offers the doubling guarantees for endocellular pH mapping. Moreover, a quantitative measurement of cellular pH changes was allowed and the endocellular pH values under drug-associated stimuli were also investigated.
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spelling pubmed-46143902015-10-29 A single design strategy for dual sensitive pH probe with a suitable range to map pH in living cells Yu, Kang-Kang Hou, Ji-Ting Li, Kun Yao, Qian Yang, Jin Wu, Ming-Yu Xie, Yong-Mei Yu, Xiao-Qi Sci Rep Article Due to the lack of a proper imaging approach, a veracious pH map of normal and abnormal cell is still rare. In this work, we presented a rhodamine-salicylaldehyde combination (Rh-SA2) as a novel pH probe, which has dual sensitive units for both acidic and basic environment. This dual sensitive probe acts like a chameleon in living cells and offers the doubling guarantees for endocellular pH mapping. Moreover, a quantitative measurement of cellular pH changes was allowed and the endocellular pH values under drug-associated stimuli were also investigated. Nature Publishing Group 2015-10-21 /pmc/articles/PMC4614390/ /pubmed/26486180 http://dx.doi.org/10.1038/srep15540 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Yu, Kang-Kang
Hou, Ji-Ting
Li, Kun
Yao, Qian
Yang, Jin
Wu, Ming-Yu
Xie, Yong-Mei
Yu, Xiao-Qi
A single design strategy for dual sensitive pH probe with a suitable range to map pH in living cells
title A single design strategy for dual sensitive pH probe with a suitable range to map pH in living cells
title_full A single design strategy for dual sensitive pH probe with a suitable range to map pH in living cells
title_fullStr A single design strategy for dual sensitive pH probe with a suitable range to map pH in living cells
title_full_unstemmed A single design strategy for dual sensitive pH probe with a suitable range to map pH in living cells
title_short A single design strategy for dual sensitive pH probe with a suitable range to map pH in living cells
title_sort single design strategy for dual sensitive ph probe with a suitable range to map ph in living cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4614390/
https://www.ncbi.nlm.nih.gov/pubmed/26486180
http://dx.doi.org/10.1038/srep15540
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