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Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast Carcinomas

The present study investigated (i) the relationship between standardised morphometric AgNOR parameters (argyrophilic nucleolar organiser region-associated proteins) and MIB1 growth fraction, and (ii) their correlation with immunohistochemical p53, sex steroid receptor status and histopathological di...

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Detalles Bibliográficos
Autores principales: Bànkfalvi, Àgnes, Schmitz, Klaus, Mock, Tobias, Kemper, Mechthild, Cubick, Christiane, Böcker, Werner
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4615186/
https://www.ncbi.nlm.nih.gov/pubmed/10391375
http://dx.doi.org/10.1155/1998/760324
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author Bànkfalvi, Àgnes
Schmitz, Klaus
Mock, Tobias
Kemper, Mechthild
Cubick, Christiane
Böcker, Werner
author_facet Bànkfalvi, Àgnes
Schmitz, Klaus
Mock, Tobias
Kemper, Mechthild
Cubick, Christiane
Böcker, Werner
author_sort Bànkfalvi, Àgnes
collection PubMed
description The present study investigated (i) the relationship between standardised morphometric AgNOR parameters (argyrophilic nucleolar organiser region-associated proteins) and MIB1 growth fraction, and (ii) their correlation with immunohistochemical p53, sex steroid receptor status and histopathological differentiation grade in serial paraffin sections from 39 breast carcinomas. Ten sections were double-stained for AgNOR/MIB1. AgNOR parameters correlated significantly with MIB1 growth fraction and p53 protein expression. Significant inverse correlation was found between proliferation markers and oestrogen/progesterone receptor status and histopathological grade. AgNOR expression was significantly higher in cycling (MIB1 positive) tumour cells, than in resting (MIB1 negative) ones, however with exceptions. We conclude, that standardised AgNOR parameters correlate with markers of increased malignant potential in breast carcinomas. However, AgNORs seem to reflect proliferation independent cellular and nucleolar activity of tumour cells, as well. We recommend the use of standardised AgNOR analysis for obtaining sound results in routine paraffin sections.
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spelling pubmed-46151862016-01-12 Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast Carcinomas Bànkfalvi, Àgnes Schmitz, Klaus Mock, Tobias Kemper, Mechthild Cubick, Christiane Böcker, Werner Anal Cell Pathol Other The present study investigated (i) the relationship between standardised morphometric AgNOR parameters (argyrophilic nucleolar organiser region-associated proteins) and MIB1 growth fraction, and (ii) their correlation with immunohistochemical p53, sex steroid receptor status and histopathological differentiation grade in serial paraffin sections from 39 breast carcinomas. Ten sections were double-stained for AgNOR/MIB1. AgNOR parameters correlated significantly with MIB1 growth fraction and p53 protein expression. Significant inverse correlation was found between proliferation markers and oestrogen/progesterone receptor status and histopathological grade. AgNOR expression was significantly higher in cycling (MIB1 positive) tumour cells, than in resting (MIB1 negative) ones, however with exceptions. We conclude, that standardised AgNOR parameters correlate with markers of increased malignant potential in breast carcinomas. However, AgNORs seem to reflect proliferation independent cellular and nucleolar activity of tumour cells, as well. We recommend the use of standardised AgNOR analysis for obtaining sound results in routine paraffin sections. IOS Press 1998 1998-01-01 /pmc/articles/PMC4615186/ /pubmed/10391375 http://dx.doi.org/10.1155/1998/760324 Text en Copyright © 1998 Hindawi Publishing Corporation.
spellingShingle Other
Bànkfalvi, Àgnes
Schmitz, Klaus
Mock, Tobias
Kemper, Mechthild
Cubick, Christiane
Böcker, Werner
Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast Carcinomas
title Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast Carcinomas
title_full Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast Carcinomas
title_fullStr Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast Carcinomas
title_full_unstemmed Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast Carcinomas
title_short Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast Carcinomas
title_sort relationship between agnor proteins, ki-67 antigen, p53 immunophenotype and differentiation markers in archival breast carcinomas
topic Other
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4615186/
https://www.ncbi.nlm.nih.gov/pubmed/10391375
http://dx.doi.org/10.1155/1998/760324
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