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RNA imaging in living cells – methods and applications
Numerous types of transcripts perform multiple functions in cells, and these functions are mainly facilitated by the interactions of the RNA with various proteins and other RNAs. Insight into the dynamics of RNA biosynthesis, processing and cellular activities is highly desirable because this knowle...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4615301/ https://www.ncbi.nlm.nih.gov/pubmed/25483044 http://dx.doi.org/10.4161/rna.35506 |
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author | Urbanek, Martyna O Galka-Marciniak, Paulina Olejniczak, Marta Krzyzosiak, Wlodzimierz J |
author_facet | Urbanek, Martyna O Galka-Marciniak, Paulina Olejniczak, Marta Krzyzosiak, Wlodzimierz J |
author_sort | Urbanek, Martyna O |
collection | PubMed |
description | Numerous types of transcripts perform multiple functions in cells, and these functions are mainly facilitated by the interactions of the RNA with various proteins and other RNAs. Insight into the dynamics of RNA biosynthesis, processing and cellular activities is highly desirable because this knowledge will deepen our understanding of cell physiology and help explain the mechanisms of RNA-mediated pathologies. In this review, we discuss the live RNA imaging systems that have been developed to date. We highlight information on the design of these systems, briefly discuss their advantages and limitations and provide examples of their numerous applications in various organisms and cell types. We present a detailed examination of one application of RNA imaging systems: this application aims to explain the role of mutant transcripts in human disease pathogenesis caused by triplet repeat expansions. Thus, this review introduces live RNA imaging systems and provides a glimpse into their various applications. |
format | Online Article Text |
id | pubmed-4615301 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-46153012015-10-31 RNA imaging in living cells – methods and applications Urbanek, Martyna O Galka-Marciniak, Paulina Olejniczak, Marta Krzyzosiak, Wlodzimierz J RNA Biol Review Numerous types of transcripts perform multiple functions in cells, and these functions are mainly facilitated by the interactions of the RNA with various proteins and other RNAs. Insight into the dynamics of RNA biosynthesis, processing and cellular activities is highly desirable because this knowledge will deepen our understanding of cell physiology and help explain the mechanisms of RNA-mediated pathologies. In this review, we discuss the live RNA imaging systems that have been developed to date. We highlight information on the design of these systems, briefly discuss their advantages and limitations and provide examples of their numerous applications in various organisms and cell types. We present a detailed examination of one application of RNA imaging systems: this application aims to explain the role of mutant transcripts in human disease pathogenesis caused by triplet repeat expansions. Thus, this review introduces live RNA imaging systems and provides a glimpse into their various applications. Taylor & Francis 2014-10-31 /pmc/articles/PMC4615301/ /pubmed/25483044 http://dx.doi.org/10.4161/rna.35506 Text en © 2014 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. |
spellingShingle | Review Urbanek, Martyna O Galka-Marciniak, Paulina Olejniczak, Marta Krzyzosiak, Wlodzimierz J RNA imaging in living cells – methods and applications |
title | RNA imaging in living cells – methods and applications |
title_full | RNA imaging in living cells – methods and applications |
title_fullStr | RNA imaging in living cells – methods and applications |
title_full_unstemmed | RNA imaging in living cells – methods and applications |
title_short | RNA imaging in living cells – methods and applications |
title_sort | rna imaging in living cells – methods and applications |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4615301/ https://www.ncbi.nlm.nih.gov/pubmed/25483044 http://dx.doi.org/10.4161/rna.35506 |
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