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Phytomedical investigation of Najas minor All. in the view of the chemical constituents
Plants are an abundant natural source of effective antibiotic compounds. Phytomedical investigations of certain plants haven't still been conducted. One of them is Najas minor (N. minor), an aquatic plant with confirmed allelopathy. Research conducted in this study showed the influence of water...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Leibniz Research Centre for Working Environment and Human Factors
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4616246/ https://www.ncbi.nlm.nih.gov/pubmed/26535038 http://dx.doi.org/10.17179/excli2014-662 |
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author | Topuzovic, Marina D. Radojevic, Ivana D. Dekic, Milan S. Radulovic, Niko S. Vasic, Sava M. Comic, Ljiljana R. Licina, Braho Z. |
author_facet | Topuzovic, Marina D. Radojevic, Ivana D. Dekic, Milan S. Radulovic, Niko S. Vasic, Sava M. Comic, Ljiljana R. Licina, Braho Z. |
author_sort | Topuzovic, Marina D. |
collection | PubMed |
description | Plants are an abundant natural source of effective antibiotic compounds. Phytomedical investigations of certain plants haven't still been conducted. One of them is Najas minor (N. minor), an aquatic plant with confirmed allelopathy. Research conducted in this study showed the influence of water and ethyl acetate extracts of N. minor on microorganisms, in the view of chemical profiling of volatile constituents and the concentrations of total phenols, flavonoids and tannins. Antimicrobial activity was defined by determining minimum inhibitory and minimum microbicidal concentrations using microdilution method. Influence on bacterial biofilm formation was performed by tissue culture plate method. The total phenolics, flavonoids and condensed tannins were determined by Folin-Ciocalteu, aluminum chloride and butanol-HCl colorimetric methods. Chemical profiling of volatile constituents was investigated by GC and GC-MS. Water extract didn't have antimicrobial activity below 5000 µg/mL. Ethyl acetate extract has shown strong antimicrobial activity on G+ bacteria - Staphylococcus aureus PMFKGB12 and Bacillus subtilis (MIC < 78.13 µg/mL). The best antibiofilm activity was obtained on Escherichia coli ATCC25922 (BIC50 at 719 µg/mL). Water extract had higher yield. Ethyl acetate extract had a significantly greater amount of total phenolics, flavonoids and tannins. As major constituent hexahydrofarnesyl acetone was identified. The ethyl acetate extract effected only G+ bacteria, but the biofilm formation of G-bacteria was suppressed. There was a connection between those in vivo and in vitro effects against pathogenic bacterial biofilm formation. All of this points to a so far unexplored potential of N. minor. |
format | Online Article Text |
id | pubmed-4616246 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Leibniz Research Centre for Working Environment and Human Factors |
record_format | MEDLINE/PubMed |
spelling | pubmed-46162462015-11-03 Phytomedical investigation of Najas minor All. in the view of the chemical constituents Topuzovic, Marina D. Radojevic, Ivana D. Dekic, Milan S. Radulovic, Niko S. Vasic, Sava M. Comic, Ljiljana R. Licina, Braho Z. EXCLI J Original Article Plants are an abundant natural source of effective antibiotic compounds. Phytomedical investigations of certain plants haven't still been conducted. One of them is Najas minor (N. minor), an aquatic plant with confirmed allelopathy. Research conducted in this study showed the influence of water and ethyl acetate extracts of N. minor on microorganisms, in the view of chemical profiling of volatile constituents and the concentrations of total phenols, flavonoids and tannins. Antimicrobial activity was defined by determining minimum inhibitory and minimum microbicidal concentrations using microdilution method. Influence on bacterial biofilm formation was performed by tissue culture plate method. The total phenolics, flavonoids and condensed tannins were determined by Folin-Ciocalteu, aluminum chloride and butanol-HCl colorimetric methods. Chemical profiling of volatile constituents was investigated by GC and GC-MS. Water extract didn't have antimicrobial activity below 5000 µg/mL. Ethyl acetate extract has shown strong antimicrobial activity on G+ bacteria - Staphylococcus aureus PMFKGB12 and Bacillus subtilis (MIC < 78.13 µg/mL). The best antibiofilm activity was obtained on Escherichia coli ATCC25922 (BIC50 at 719 µg/mL). Water extract had higher yield. Ethyl acetate extract had a significantly greater amount of total phenolics, flavonoids and tannins. As major constituent hexahydrofarnesyl acetone was identified. The ethyl acetate extract effected only G+ bacteria, but the biofilm formation of G-bacteria was suppressed. There was a connection between those in vivo and in vitro effects against pathogenic bacterial biofilm formation. All of this points to a so far unexplored potential of N. minor. Leibniz Research Centre for Working Environment and Human Factors 2015-03-30 /pmc/articles/PMC4616246/ /pubmed/26535038 http://dx.doi.org/10.17179/excli2014-662 Text en Copyright © 2015 Topuzovic et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/) You are free to copy, distribute and transmit the work, provided the original author and source are credited. |
spellingShingle | Original Article Topuzovic, Marina D. Radojevic, Ivana D. Dekic, Milan S. Radulovic, Niko S. Vasic, Sava M. Comic, Ljiljana R. Licina, Braho Z. Phytomedical investigation of Najas minor All. in the view of the chemical constituents |
title | Phytomedical investigation of Najas minor All. in the view of the chemical constituents |
title_full | Phytomedical investigation of Najas minor All. in the view of the chemical constituents |
title_fullStr | Phytomedical investigation of Najas minor All. in the view of the chemical constituents |
title_full_unstemmed | Phytomedical investigation of Najas minor All. in the view of the chemical constituents |
title_short | Phytomedical investigation of Najas minor All. in the view of the chemical constituents |
title_sort | phytomedical investigation of najas minor all. in the view of the chemical constituents |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4616246/ https://www.ncbi.nlm.nih.gov/pubmed/26535038 http://dx.doi.org/10.17179/excli2014-662 |
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