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Phytomedical investigation of Najas minor All. in the view of the chemical constituents

Plants are an abundant natural source of effective antibiotic compounds. Phytomedical investigations of certain plants haven't still been conducted. One of them is Najas minor (N. minor), an aquatic plant with confirmed allelopathy. Research conducted in this study showed the influence of water...

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Autores principales: Topuzovic, Marina D., Radojevic, Ivana D., Dekic, Milan S., Radulovic, Niko S., Vasic, Sava M., Comic, Ljiljana R., Licina, Braho Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Leibniz Research Centre for Working Environment and Human Factors 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4616246/
https://www.ncbi.nlm.nih.gov/pubmed/26535038
http://dx.doi.org/10.17179/excli2014-662
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author Topuzovic, Marina D.
Radojevic, Ivana D.
Dekic, Milan S.
Radulovic, Niko S.
Vasic, Sava M.
Comic, Ljiljana R.
Licina, Braho Z.
author_facet Topuzovic, Marina D.
Radojevic, Ivana D.
Dekic, Milan S.
Radulovic, Niko S.
Vasic, Sava M.
Comic, Ljiljana R.
Licina, Braho Z.
author_sort Topuzovic, Marina D.
collection PubMed
description Plants are an abundant natural source of effective antibiotic compounds. Phytomedical investigations of certain plants haven't still been conducted. One of them is Najas minor (N. minor), an aquatic plant with confirmed allelopathy. Research conducted in this study showed the influence of water and ethyl acetate extracts of N. minor on microorganisms, in the view of chemical profiling of volatile constituents and the concentrations of total phenols, flavonoids and tannins. Antimicrobial activity was defined by determining minimum inhibitory and minimum microbicidal concentrations using microdilution method. Influence on bacterial biofilm formation was performed by tissue culture plate method. The total phenolics, flavonoids and condensed tannins were determined by Folin-Ciocalteu, aluminum chloride and butanol-HCl colorimetric methods. Chemical profiling of volatile constituents was investigated by GC and GC-MS. Water extract didn't have antimicrobial activity below 5000 µg/mL. Ethyl acetate extract has shown strong antimicrobial activity on G+ bacteria - Staphylococcus aureus PMFKGB12 and Bacillus subtilis (MIC < 78.13 µg/mL). The best antibiofilm activity was obtained on Escherichia coli ATCC25922 (BIC50 at 719 µg/mL). Water extract had higher yield. Ethyl acetate extract had a significantly greater amount of total phenolics, flavonoids and tannins. As major constituent hexahydrofarnesyl acetone was identified. The ethyl acetate extract effected only G+ bacteria, but the biofilm formation of G-bacteria was suppressed. There was a connection between those in vivo and in vitro effects against pathogenic bacterial biofilm formation. All of this points to a so far unexplored potential of N. minor.
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spelling pubmed-46162462015-11-03 Phytomedical investigation of Najas minor All. in the view of the chemical constituents Topuzovic, Marina D. Radojevic, Ivana D. Dekic, Milan S. Radulovic, Niko S. Vasic, Sava M. Comic, Ljiljana R. Licina, Braho Z. EXCLI J Original Article Plants are an abundant natural source of effective antibiotic compounds. Phytomedical investigations of certain plants haven't still been conducted. One of them is Najas minor (N. minor), an aquatic plant with confirmed allelopathy. Research conducted in this study showed the influence of water and ethyl acetate extracts of N. minor on microorganisms, in the view of chemical profiling of volatile constituents and the concentrations of total phenols, flavonoids and tannins. Antimicrobial activity was defined by determining minimum inhibitory and minimum microbicidal concentrations using microdilution method. Influence on bacterial biofilm formation was performed by tissue culture plate method. The total phenolics, flavonoids and condensed tannins were determined by Folin-Ciocalteu, aluminum chloride and butanol-HCl colorimetric methods. Chemical profiling of volatile constituents was investigated by GC and GC-MS. Water extract didn't have antimicrobial activity below 5000 µg/mL. Ethyl acetate extract has shown strong antimicrobial activity on G+ bacteria - Staphylococcus aureus PMFKGB12 and Bacillus subtilis (MIC < 78.13 µg/mL). The best antibiofilm activity was obtained on Escherichia coli ATCC25922 (BIC50 at 719 µg/mL). Water extract had higher yield. Ethyl acetate extract had a significantly greater amount of total phenolics, flavonoids and tannins. As major constituent hexahydrofarnesyl acetone was identified. The ethyl acetate extract effected only G+ bacteria, but the biofilm formation of G-bacteria was suppressed. There was a connection between those in vivo and in vitro effects against pathogenic bacterial biofilm formation. All of this points to a so far unexplored potential of N. minor. Leibniz Research Centre for Working Environment and Human Factors 2015-03-30 /pmc/articles/PMC4616246/ /pubmed/26535038 http://dx.doi.org/10.17179/excli2014-662 Text en Copyright © 2015 Topuzovic et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/) You are free to copy, distribute and transmit the work, provided the original author and source are credited.
spellingShingle Original Article
Topuzovic, Marina D.
Radojevic, Ivana D.
Dekic, Milan S.
Radulovic, Niko S.
Vasic, Sava M.
Comic, Ljiljana R.
Licina, Braho Z.
Phytomedical investigation of Najas minor All. in the view of the chemical constituents
title Phytomedical investigation of Najas minor All. in the view of the chemical constituents
title_full Phytomedical investigation of Najas minor All. in the view of the chemical constituents
title_fullStr Phytomedical investigation of Najas minor All. in the view of the chemical constituents
title_full_unstemmed Phytomedical investigation of Najas minor All. in the view of the chemical constituents
title_short Phytomedical investigation of Najas minor All. in the view of the chemical constituents
title_sort phytomedical investigation of najas minor all. in the view of the chemical constituents
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4616246/
https://www.ncbi.nlm.nih.gov/pubmed/26535038
http://dx.doi.org/10.17179/excli2014-662
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