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Sporothrix schenckii COMPLEX: SUSCEPTIBILITIES TO COMBINED ANTIFUNGAL AGENTS AND CHARACTERIZATION OF ENZYMATIC PROFILES
Sporothrix schenckiiwas reclassified as a complex encompassing six cryptic species, which calls for the reassessment of clinical and epidemiological data of these new species. We evaluated the susceptibility of Sporothrix albicans (n = 1) , S. brasiliensis (n = 6) , S. globosa (n = 1), S. mexicana(n...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Instituto de Medicina Tropical
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4616912/ https://www.ncbi.nlm.nih.gov/pubmed/26422151 http://dx.doi.org/10.1590/S0036-46652015000400003 |
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author | OLIVEIRA, Daniele Carvalho de LORETO, Érico Silva MARIO, Débora Alves Nunes LOPES, Paulo G. Markus NEVES, Louise Vignolles da ROCHA, Marta Pires SANTURIO, Janio Morais ALVES, Sydney Hartz |
author_facet | OLIVEIRA, Daniele Carvalho de LORETO, Érico Silva MARIO, Débora Alves Nunes LOPES, Paulo G. Markus NEVES, Louise Vignolles da ROCHA, Marta Pires SANTURIO, Janio Morais ALVES, Sydney Hartz |
author_sort | OLIVEIRA, Daniele Carvalho |
collection | PubMed |
description | Sporothrix schenckiiwas reclassified as a complex encompassing six cryptic species, which calls for the reassessment of clinical and epidemiological data of these new species. We evaluated the susceptibility of Sporothrix albicans (n = 1) , S. brasiliensis (n = 6) , S. globosa (n = 1), S. mexicana(n = 1) and S. schenckii(n = 36) to terbinafine (TRB) alone and in combination with itraconazole (ITZ), ketoconazole (KTZ), and voriconazole (VRZ) by a checkerboard microdilution method and determined the enzymatic profile of these species with the API-ZYM kit. Most interactions were additive (27.5%, 32.5% and 5%) or indifferent (70%, 50% and 52.5%) for TRB+KTZ, TRB+ITZ and TRB+VRZ, respectively. Antagonisms were observed in 42.5% of isolates for the TRB+VRZ combination. Based on enzymatic profiling, the Sporothrix schenckii strains were categorized into 14 biotypes. Leucine arylamidase (LA) activity was observed only for S. albicans and S. mexicana. The species S. globosaand S. mexicanawere the only species without β-glucosidase (GS) activity. Our results may contribute to a better understanding of virulence and resistance among species of the genus Sporothrixin further studies. |
format | Online Article Text |
id | pubmed-4616912 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Instituto de Medicina Tropical |
record_format | MEDLINE/PubMed |
spelling | pubmed-46169122015-11-03 Sporothrix schenckii COMPLEX: SUSCEPTIBILITIES TO COMBINED ANTIFUNGAL AGENTS AND CHARACTERIZATION OF ENZYMATIC PROFILES OLIVEIRA, Daniele Carvalho de LORETO, Érico Silva MARIO, Débora Alves Nunes LOPES, Paulo G. Markus NEVES, Louise Vignolles da ROCHA, Marta Pires SANTURIO, Janio Morais ALVES, Sydney Hartz Rev Inst Med Trop Sao Paulo Mycology Sporothrix schenckiiwas reclassified as a complex encompassing six cryptic species, which calls for the reassessment of clinical and epidemiological data of these new species. We evaluated the susceptibility of Sporothrix albicans (n = 1) , S. brasiliensis (n = 6) , S. globosa (n = 1), S. mexicana(n = 1) and S. schenckii(n = 36) to terbinafine (TRB) alone and in combination with itraconazole (ITZ), ketoconazole (KTZ), and voriconazole (VRZ) by a checkerboard microdilution method and determined the enzymatic profile of these species with the API-ZYM kit. Most interactions were additive (27.5%, 32.5% and 5%) or indifferent (70%, 50% and 52.5%) for TRB+KTZ, TRB+ITZ and TRB+VRZ, respectively. Antagonisms were observed in 42.5% of isolates for the TRB+VRZ combination. Based on enzymatic profiling, the Sporothrix schenckii strains were categorized into 14 biotypes. Leucine arylamidase (LA) activity was observed only for S. albicans and S. mexicana. The species S. globosaand S. mexicanawere the only species without β-glucosidase (GS) activity. Our results may contribute to a better understanding of virulence and resistance among species of the genus Sporothrixin further studies. Instituto de Medicina Tropical 2015 /pmc/articles/PMC4616912/ /pubmed/26422151 http://dx.doi.org/10.1590/S0036-46652015000400003 Text en http://creativecommons.org/licenses/by-nc/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License |
spellingShingle | Mycology OLIVEIRA, Daniele Carvalho de LORETO, Érico Silva MARIO, Débora Alves Nunes LOPES, Paulo G. Markus NEVES, Louise Vignolles da ROCHA, Marta Pires SANTURIO, Janio Morais ALVES, Sydney Hartz Sporothrix schenckii COMPLEX: SUSCEPTIBILITIES TO COMBINED ANTIFUNGAL AGENTS AND CHARACTERIZATION OF ENZYMATIC PROFILES |
title | Sporothrix schenckii COMPLEX: SUSCEPTIBILITIES TO COMBINED ANTIFUNGAL AGENTS AND CHARACTERIZATION OF ENZYMATIC PROFILES |
title_full | Sporothrix schenckii COMPLEX: SUSCEPTIBILITIES TO COMBINED ANTIFUNGAL AGENTS AND CHARACTERIZATION OF ENZYMATIC PROFILES |
title_fullStr | Sporothrix schenckii COMPLEX: SUSCEPTIBILITIES TO COMBINED ANTIFUNGAL AGENTS AND CHARACTERIZATION OF ENZYMATIC PROFILES |
title_full_unstemmed | Sporothrix schenckii COMPLEX: SUSCEPTIBILITIES TO COMBINED ANTIFUNGAL AGENTS AND CHARACTERIZATION OF ENZYMATIC PROFILES |
title_short | Sporothrix schenckii COMPLEX: SUSCEPTIBILITIES TO COMBINED ANTIFUNGAL AGENTS AND CHARACTERIZATION OF ENZYMATIC PROFILES |
title_sort | sporothrix schenckii complex: susceptibilities to combined antifungal agents and characterization of enzymatic profiles |
topic | Mycology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4616912/ https://www.ncbi.nlm.nih.gov/pubmed/26422151 http://dx.doi.org/10.1590/S0036-46652015000400003 |
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