DIARRHEA OUTBREAK IN PERNAMBUCO, BRAZIL, ASSOCIATED WITH A HEAT-STABLE CYTOTOXIC ENTEROTOXIN PRODUCED BY Aeromonas caviae

In the present study enterotoxic and cytotoxic activities of twenty Aeromonas caviaestrains were examined. They originated from fecal specimens of patients with acute diarrhea during an outbreak in Brazil in 2004. Culture supernatants of fourteen strains (70%) caused fluid accumulation in rabbit ile...

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Autores principales: LOPES, Ana Carolina Amaral, MARTINS, Luciano Moura, GATTI, Maria Silvia Viccari, FALAVINA DOS REIS, Cristhiane Moura, HOFER, Ernesto, YANO, Tomomasa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2015
Materias:
Bacteriology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4616922/
https://www.ncbi.nlm.nih.gov/pubmed/26422161
http://dx.doi.org/10.1590/S0036-46652015000400013
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author LOPES, Ana Carolina Amaral
MARTINS, Luciano Moura
GATTI, Maria Silvia Viccari
FALAVINA DOS REIS, Cristhiane Moura
HOFER, Ernesto
YANO, Tomomasa
author_facet LOPES, Ana Carolina Amaral
MARTINS, Luciano Moura
GATTI, Maria Silvia Viccari
FALAVINA DOS REIS, Cristhiane Moura
HOFER, Ernesto
YANO, Tomomasa
author_sort LOPES, Ana Carolina Amaral
collection PubMed
description In the present study enterotoxic and cytotoxic activities of twenty Aeromonas caviaestrains were examined. They originated from fecal specimens of patients with acute diarrhea during an outbreak in Brazil in 2004. Culture supernatants of fourteen strains (70%) caused fluid accumulation in rabbit ileal intestinal loops and in suckling mice assays, and also showed a cytotoxic activity in Vero and Caco-2 cells. The enterotoxic and cytotoxic factors were heat-stable after culture supernatants treatment at 100 ºC. The results revealed that A. caviaestrains produce a putative diarrheagenic virulence factor, a heat-stable cytotoxic enterotoxin that could be linked to the diarrhea outbreak that took place in Brazil.
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spelling pubmed-46169222015-11-03 DIARRHEA OUTBREAK IN PERNAMBUCO, BRAZIL, ASSOCIATED WITH A HEAT-STABLE CYTOTOXIC ENTEROTOXIN PRODUCED BY Aeromonas caviae LOPES, Ana Carolina Amaral MARTINS, Luciano Moura GATTI, Maria Silvia Viccari FALAVINA DOS REIS, Cristhiane Moura HOFER, Ernesto YANO, Tomomasa Rev Inst Med Trop Sao Paulo Bacteriology In the present study enterotoxic and cytotoxic activities of twenty Aeromonas caviaestrains were examined. They originated from fecal specimens of patients with acute diarrhea during an outbreak in Brazil in 2004. Culture supernatants of fourteen strains (70%) caused fluid accumulation in rabbit ileal intestinal loops and in suckling mice assays, and also showed a cytotoxic activity in Vero and Caco-2 cells. The enterotoxic and cytotoxic factors were heat-stable after culture supernatants treatment at 100 ºC. The results revealed that A. caviaestrains produce a putative diarrheagenic virulence factor, a heat-stable cytotoxic enterotoxin that could be linked to the diarrhea outbreak that took place in Brazil. Instituto de Medicina Tropical 2015 /pmc/articles/PMC4616922/ /pubmed/26422161 http://dx.doi.org/10.1590/S0036-46652015000400013 Text en http://creativecommons.org/licenses/by-nc/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Bacteriology
LOPES, Ana Carolina Amaral
MARTINS, Luciano Moura
GATTI, Maria Silvia Viccari
FALAVINA DOS REIS, Cristhiane Moura
HOFER, Ernesto
YANO, Tomomasa
DIARRHEA OUTBREAK IN PERNAMBUCO, BRAZIL, ASSOCIATED WITH A HEAT-STABLE CYTOTOXIC ENTEROTOXIN PRODUCED BY Aeromonas caviae
title DIARRHEA OUTBREAK IN PERNAMBUCO, BRAZIL, ASSOCIATED WITH A HEAT-STABLE CYTOTOXIC ENTEROTOXIN PRODUCED BY Aeromonas caviae
title_full DIARRHEA OUTBREAK IN PERNAMBUCO, BRAZIL, ASSOCIATED WITH A HEAT-STABLE CYTOTOXIC ENTEROTOXIN PRODUCED BY Aeromonas caviae
title_fullStr DIARRHEA OUTBREAK IN PERNAMBUCO, BRAZIL, ASSOCIATED WITH A HEAT-STABLE CYTOTOXIC ENTEROTOXIN PRODUCED BY Aeromonas caviae
title_full_unstemmed DIARRHEA OUTBREAK IN PERNAMBUCO, BRAZIL, ASSOCIATED WITH A HEAT-STABLE CYTOTOXIC ENTEROTOXIN PRODUCED BY Aeromonas caviae
title_short DIARRHEA OUTBREAK IN PERNAMBUCO, BRAZIL, ASSOCIATED WITH A HEAT-STABLE CYTOTOXIC ENTEROTOXIN PRODUCED BY Aeromonas caviae
title_sort diarrhea outbreak in pernambuco, brazil, associated with a heat-stable cytotoxic enterotoxin produced by aeromonas caviae
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4616922/
https://www.ncbi.nlm.nih.gov/pubmed/26422161
http://dx.doi.org/10.1590/S0036-46652015000400013
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