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Fluoroquinolone Resistance Mechanisms and population structure of Enterobacter cloacae non-susceptible to Ertapenem in North-Eastern France
Fluoroquinolone (FQ) agents are a potential resort to treat infection due to Enterobacteriaceae producing extended spectrum β-lactamase and susceptible to FQ. In a context of increase of non-susceptibility to carbapenems among Enterobacteriaceae, we characterized FQ resistance mechanisms in 75 Enter...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4616961/ https://www.ncbi.nlm.nih.gov/pubmed/26557115 http://dx.doi.org/10.3389/fmicb.2015.01186 |
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author | Guillard, Thomas Cholley, Pascal Limelette, Anne Hocquet, Didier Matton, Lucie Guyeux, Christophe Lebreil, Anne-Laure Bajolet, Odile Brasme, Lucien Madoux, Janick Vernet-Garnier, Véronique Barbe, Coralie Bertrand, Xavier de Champs on behalf of CarbaFrEst Group, Christophe |
author_facet | Guillard, Thomas Cholley, Pascal Limelette, Anne Hocquet, Didier Matton, Lucie Guyeux, Christophe Lebreil, Anne-Laure Bajolet, Odile Brasme, Lucien Madoux, Janick Vernet-Garnier, Véronique Barbe, Coralie Bertrand, Xavier de Champs on behalf of CarbaFrEst Group, Christophe |
author_sort | Guillard, Thomas |
collection | PubMed |
description | Fluoroquinolone (FQ) agents are a potential resort to treat infection due to Enterobacteriaceae producing extended spectrum β-lactamase and susceptible to FQ. In a context of increase of non-susceptibility to carbapenems among Enterobacteriaceae, we characterized FQ resistance mechanisms in 75 Enterobacter cloacae isolates non-susceptible to ertapenem in North-Eastern France in 2012 and describe the population structure by pulsed field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Among them, 14.7% (12/75) carried a carbapenemase-encoding gene. Except one isolate producing VIM-1, the carbapenemase-producing isolates carried the well-known IncL/M pOXA48a plasmid. Most of the isolates (59/75) harbored at least a FQ-R determinant. qnr genes were predominant (40%, 30/75). The MLST study revealed that E. cloacae isolates’ clonality was wide [24 different sequence types (STs)]. The more widespread STs were ST74, ST101, ST110, ST114, and ST133. Carbapenem MICs were higher for E. cloacae ST74 than for other E. cloacae isolates. Plasmid-mediated quinolone resistance determinants were more often observed in E. cloacae ST74 isolates. These findings showed that (i) pOXA-48a is spreading in North-Eastern France, (ii) qnr is preponderant in E. cloacae, (iii) E. cloacae comprised a large amount of lineages spreading in North-Eastern France, and (iv) FQ as an alternative to β-lactams to treat ertapenem non-susceptible Enterobacteriaceae are compromised. |
format | Online Article Text |
id | pubmed-4616961 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-46169612015-11-09 Fluoroquinolone Resistance Mechanisms and population structure of Enterobacter cloacae non-susceptible to Ertapenem in North-Eastern France Guillard, Thomas Cholley, Pascal Limelette, Anne Hocquet, Didier Matton, Lucie Guyeux, Christophe Lebreil, Anne-Laure Bajolet, Odile Brasme, Lucien Madoux, Janick Vernet-Garnier, Véronique Barbe, Coralie Bertrand, Xavier de Champs on behalf of CarbaFrEst Group, Christophe Front Microbiol Microbiology Fluoroquinolone (FQ) agents are a potential resort to treat infection due to Enterobacteriaceae producing extended spectrum β-lactamase and susceptible to FQ. In a context of increase of non-susceptibility to carbapenems among Enterobacteriaceae, we characterized FQ resistance mechanisms in 75 Enterobacter cloacae isolates non-susceptible to ertapenem in North-Eastern France in 2012 and describe the population structure by pulsed field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Among them, 14.7% (12/75) carried a carbapenemase-encoding gene. Except one isolate producing VIM-1, the carbapenemase-producing isolates carried the well-known IncL/M pOXA48a plasmid. Most of the isolates (59/75) harbored at least a FQ-R determinant. qnr genes were predominant (40%, 30/75). The MLST study revealed that E. cloacae isolates’ clonality was wide [24 different sequence types (STs)]. The more widespread STs were ST74, ST101, ST110, ST114, and ST133. Carbapenem MICs were higher for E. cloacae ST74 than for other E. cloacae isolates. Plasmid-mediated quinolone resistance determinants were more often observed in E. cloacae ST74 isolates. These findings showed that (i) pOXA-48a is spreading in North-Eastern France, (ii) qnr is preponderant in E. cloacae, (iii) E. cloacae comprised a large amount of lineages spreading in North-Eastern France, and (iv) FQ as an alternative to β-lactams to treat ertapenem non-susceptible Enterobacteriaceae are compromised. Frontiers Media S.A. 2015-10-23 /pmc/articles/PMC4616961/ /pubmed/26557115 http://dx.doi.org/10.3389/fmicb.2015.01186 Text en Copyright © 2015 Guillard, Cholley, Limelette, Hocquet, Matton, Guyeux, Lebreil, Bajolet, Brasme, Madoux, Vernet-Garnier, Barbe, Bertrand and de Champs on behalf of CarbaFrEst Group. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Guillard, Thomas Cholley, Pascal Limelette, Anne Hocquet, Didier Matton, Lucie Guyeux, Christophe Lebreil, Anne-Laure Bajolet, Odile Brasme, Lucien Madoux, Janick Vernet-Garnier, Véronique Barbe, Coralie Bertrand, Xavier de Champs on behalf of CarbaFrEst Group, Christophe Fluoroquinolone Resistance Mechanisms and population structure of Enterobacter cloacae non-susceptible to Ertapenem in North-Eastern France |
title | Fluoroquinolone Resistance Mechanisms and population structure of Enterobacter cloacae non-susceptible to Ertapenem in North-Eastern France |
title_full | Fluoroquinolone Resistance Mechanisms and population structure of Enterobacter cloacae non-susceptible to Ertapenem in North-Eastern France |
title_fullStr | Fluoroquinolone Resistance Mechanisms and population structure of Enterobacter cloacae non-susceptible to Ertapenem in North-Eastern France |
title_full_unstemmed | Fluoroquinolone Resistance Mechanisms and population structure of Enterobacter cloacae non-susceptible to Ertapenem in North-Eastern France |
title_short | Fluoroquinolone Resistance Mechanisms and population structure of Enterobacter cloacae non-susceptible to Ertapenem in North-Eastern France |
title_sort | fluoroquinolone resistance mechanisms and population structure of enterobacter cloacae non-susceptible to ertapenem in north-eastern france |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4616961/ https://www.ncbi.nlm.nih.gov/pubmed/26557115 http://dx.doi.org/10.3389/fmicb.2015.01186 |
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