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Nucleotide excision repair pathway assessment in DNA exposed to low-intensity red and infrared lasers
Low-intensity lasers are used for prevention and management of oral mucositis induced by anticancer therapy, but the effectiveness of treatment depends on the genetic characteristics of affected cells. This study evaluated the survival and induction of filamentation of Escherichia coli cells deficie...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Associação Brasileira de Divulgação Científica
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4617120/ https://www.ncbi.nlm.nih.gov/pubmed/26445337 http://dx.doi.org/10.1590/1414-431X20154457 |
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author | Fonseca, A.S. Campos, V.M.A. Magalhães, L.A.G. Paoli, F. |
author_facet | Fonseca, A.S. Campos, V.M.A. Magalhães, L.A.G. Paoli, F. |
author_sort | Fonseca, A.S. |
collection | PubMed |
description | Low-intensity lasers are used for prevention and management of oral mucositis induced by anticancer therapy, but the effectiveness of treatment depends on the genetic characteristics of affected cells. This study evaluated the survival and induction of filamentation of Escherichia coli cells deficient in the nucleotide excision repair pathway, and the action of T(4)endonuclease V on plasmid DNA exposed to low-intensity red and near-infrared laser light. Cultures of wild-type (strain AB1157) E. coli and strain AB1886 (deficient in uvrA protein) were exposed to red (660 nm) and infrared (808 nm) lasers at various fluences, powers and emission modes to study bacterial survival and filamentation. Also, plasmid DNA was exposed to laser light to study DNA lesions produced in vitro by T(4)endonuclease V. Low-intensity lasers:i) had no effect on survival of wild-type E. coli but decreased the survival of uvrA protein-deficient cells,ii) induced bacterial filamentation, iii) did not alter the electrophoretic profile of plasmids in agarose gels, andiv) did not alter the electrophoretic profile of plasmids incubated with T(4) endonuclease V. These results increase our understanding of the effects of laser light on cells with various genetic characteristics, such as xeroderma pigmentosum cells deficient in nucleotide excision pathway activity in patients with mucositis treated by low-intensity lasers. |
format | Online Article Text |
id | pubmed-4617120 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Associação Brasileira de Divulgação Científica |
record_format | MEDLINE/PubMed |
spelling | pubmed-46171202015-11-24 Nucleotide excision repair pathway assessment in DNA exposed to low-intensity red and infrared lasers Fonseca, A.S. Campos, V.M.A. Magalhães, L.A.G. Paoli, F. Braz J Med Biol Res IX Meeting of the "Sociedade Brasileira De Biociências Nucleares". Caxambu, Mg, Brazil August 27-30, 2014 Low-intensity lasers are used for prevention and management of oral mucositis induced by anticancer therapy, but the effectiveness of treatment depends on the genetic characteristics of affected cells. This study evaluated the survival and induction of filamentation of Escherichia coli cells deficient in the nucleotide excision repair pathway, and the action of T(4)endonuclease V on plasmid DNA exposed to low-intensity red and near-infrared laser light. Cultures of wild-type (strain AB1157) E. coli and strain AB1886 (deficient in uvrA protein) were exposed to red (660 nm) and infrared (808 nm) lasers at various fluences, powers and emission modes to study bacterial survival and filamentation. Also, plasmid DNA was exposed to laser light to study DNA lesions produced in vitro by T(4)endonuclease V. Low-intensity lasers:i) had no effect on survival of wild-type E. coli but decreased the survival of uvrA protein-deficient cells,ii) induced bacterial filamentation, iii) did not alter the electrophoretic profile of plasmids in agarose gels, andiv) did not alter the electrophoretic profile of plasmids incubated with T(4) endonuclease V. These results increase our understanding of the effects of laser light on cells with various genetic characteristics, such as xeroderma pigmentosum cells deficient in nucleotide excision pathway activity in patients with mucositis treated by low-intensity lasers. Associação Brasileira de Divulgação Científica 2015-07-10 /pmc/articles/PMC4617120/ /pubmed/26445337 http://dx.doi.org/10.1590/1414-431X20154457 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | IX Meeting of the "Sociedade Brasileira De Biociências Nucleares". Caxambu, Mg, Brazil August 27-30, 2014 Fonseca, A.S. Campos, V.M.A. Magalhães, L.A.G. Paoli, F. Nucleotide excision repair pathway assessment in DNA exposed to low-intensity red and infrared lasers |
title | Nucleotide excision repair pathway assessment in DNA exposed to
low-intensity red and infrared lasers |
title_full | Nucleotide excision repair pathway assessment in DNA exposed to
low-intensity red and infrared lasers |
title_fullStr | Nucleotide excision repair pathway assessment in DNA exposed to
low-intensity red and infrared lasers |
title_full_unstemmed | Nucleotide excision repair pathway assessment in DNA exposed to
low-intensity red and infrared lasers |
title_short | Nucleotide excision repair pathway assessment in DNA exposed to
low-intensity red and infrared lasers |
title_sort | nucleotide excision repair pathway assessment in dna exposed to
low-intensity red and infrared lasers |
topic | IX Meeting of the "Sociedade Brasileira De Biociências Nucleares". Caxambu, Mg, Brazil August 27-30, 2014 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4617120/ https://www.ncbi.nlm.nih.gov/pubmed/26445337 http://dx.doi.org/10.1590/1414-431X20154457 |
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