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TNFα–308G/A Polymorphism as a Risk Factor for HPV Associated Cervical Cancer in Indian Population
Background: Investigation of the potential association of single nucleotide polymorphisms (SNPs) at –308 G/A and –238 G/A of Tumor necrosis factor α (TNFα) with susceptibility to HPV-16 associated cervical cancer in Indian women. Methods: The study included 165 histologically confirmed cases with 45...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
IOS Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4617819/ https://www.ncbi.nlm.nih.gov/pubmed/17452777 http://dx.doi.org/10.1155/2007/418247 |
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author | Kohaar, Indu Thakur, Nisha Salhan, Sudha Batra, Swaraj Singh, Veena Sharma, Anita Sodhani, Pushpa Das, B. C. Sarkar, Debi P. Bharadwaj, Mausumi |
author_facet | Kohaar, Indu Thakur, Nisha Salhan, Sudha Batra, Swaraj Singh, Veena Sharma, Anita Sodhani, Pushpa Das, B. C. Sarkar, Debi P. Bharadwaj, Mausumi |
author_sort | Kohaar, Indu |
collection | PubMed |
description | Background: Investigation of the potential association of single nucleotide polymorphisms (SNPs) at –308 G/A and –238 G/A of Tumor necrosis factor α (TNFα) with susceptibility to HPV-16 associated cervical cancer in Indian women. Methods: The study included 165 histologically confirmed cases with 45 precancer and 120 cancer patients and an equal number (165) of healthy controls with normal cervical cytology. PCR-RFLP was employed to analyze TNFα promoter polymorphisms, which were confirmed by direct sequencing. Both patients and controls were screened for Human Papillomavirus (HPV) infection. Results: The frequency of –308 A allele in TNFα was significantly higher in cases compared with control subjects (21% in cases vs. 9% in controls; p < 0.01), with an odds ratio of 2.7 (95% CI = 1.41–5.15). Also, women carrying A allele for this locus presented 3 times increased susceptibility to HPV 16 infection as evident from carrier genotype distribution between HPV positive cases and control subjects (24% in HPV positive cases vs. 9% in controls; p < 0.01; OR = 3.1; 95% CI = 1.60–6.03). No such association was found for TNFα–238 (G/A) polymorphism with the risk of development of cervical cancer. Conclusion: It suggests that SNP at –308 (G/A) of TNFα promoter may represent an increased risk for HPV infection and development of cervical cancer in Indian women. |
format | Online Article Text |
id | pubmed-4617819 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | IOS Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-46178192016-01-12 TNFα–308G/A Polymorphism as a Risk Factor for HPV Associated Cervical Cancer in Indian Population Kohaar, Indu Thakur, Nisha Salhan, Sudha Batra, Swaraj Singh, Veena Sharma, Anita Sodhani, Pushpa Das, B. C. Sarkar, Debi P. Bharadwaj, Mausumi Cell Oncol Other Background: Investigation of the potential association of single nucleotide polymorphisms (SNPs) at –308 G/A and –238 G/A of Tumor necrosis factor α (TNFα) with susceptibility to HPV-16 associated cervical cancer in Indian women. Methods: The study included 165 histologically confirmed cases with 45 precancer and 120 cancer patients and an equal number (165) of healthy controls with normal cervical cytology. PCR-RFLP was employed to analyze TNFα promoter polymorphisms, which were confirmed by direct sequencing. Both patients and controls were screened for Human Papillomavirus (HPV) infection. Results: The frequency of –308 A allele in TNFα was significantly higher in cases compared with control subjects (21% in cases vs. 9% in controls; p < 0.01), with an odds ratio of 2.7 (95% CI = 1.41–5.15). Also, women carrying A allele for this locus presented 3 times increased susceptibility to HPV 16 infection as evident from carrier genotype distribution between HPV positive cases and control subjects (24% in HPV positive cases vs. 9% in controls; p < 0.01; OR = 3.1; 95% CI = 1.60–6.03). No such association was found for TNFα–238 (G/A) polymorphism with the risk of development of cervical cancer. Conclusion: It suggests that SNP at –308 (G/A) of TNFα promoter may represent an increased risk for HPV infection and development of cervical cancer in Indian women. IOS Press 2007 2007-04-23 /pmc/articles/PMC4617819/ /pubmed/17452777 http://dx.doi.org/10.1155/2007/418247 Text en Copyright © 2007 Hindawi Publishing Corporation and the authors. |
spellingShingle | Other Kohaar, Indu Thakur, Nisha Salhan, Sudha Batra, Swaraj Singh, Veena Sharma, Anita Sodhani, Pushpa Das, B. C. Sarkar, Debi P. Bharadwaj, Mausumi TNFα–308G/A Polymorphism as a Risk Factor for HPV Associated Cervical Cancer in Indian Population |
title | TNFα–308G/A Polymorphism as a Risk Factor for HPV Associated Cervical Cancer in Indian Population |
title_full | TNFα–308G/A Polymorphism as a Risk Factor for HPV Associated Cervical Cancer in Indian Population |
title_fullStr | TNFα–308G/A Polymorphism as a Risk Factor for HPV Associated Cervical Cancer in Indian Population |
title_full_unstemmed | TNFα–308G/A Polymorphism as a Risk Factor for HPV Associated Cervical Cancer in Indian Population |
title_short | TNFα–308G/A Polymorphism as a Risk Factor for HPV Associated Cervical Cancer in Indian Population |
title_sort | tnfα–308g/a polymorphism as a risk factor for hpv associated cervical cancer in indian population |
topic | Other |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4617819/ https://www.ncbi.nlm.nih.gov/pubmed/17452777 http://dx.doi.org/10.1155/2007/418247 |
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