In Situ Reverse Transcriptase-Nested Polymerase Chain Reaction to Identify Intracellular Nucleic Acids without the Necessity of Dnase Pretreatment and Hybridisation

In the present study a protocol of in situ reverse transcriptase‐nested polymerase chain reaction (in situ RT‐nested PCR) was examined based on the following modifications. (i) To exclude false positive signals caused by “DNA repair mechanisms” and “endogenous priming”, a two‐step PCR was applied af...

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Detalles Bibliográficos
Autores principales: Menschikowski, Mario, Vogel, Margot, Eckey, Rolf, Dinnebier, Gerd, Jaross, Werner
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2001
Materias:
Other
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618000/
https://www.ncbi.nlm.nih.gov/pubmed/11455034
http://dx.doi.org/10.1155/2001/654016

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