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Telomerase Activity Levels in the Surgical Margin and Tumour Distant Tissue of the Squamous Cell Carcinoma of the Head-And-Neck

The survival of patients with a head‐and‐neck squamous cell carcinoma is determined by loco‐regional recurrence and second primary carcinomas. As a complement to histopathology, molecular changes of tumour marginal and tumour distant tissue may confirm curative surgical tumour extirpation. We tested...

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Detalles Bibliográficos
Autores principales: Fabricius, Eva‐Maria, Gurr, Ulrike, Wildner, Gustav‐Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618224/
https://www.ncbi.nlm.nih.gov/pubmed/12122281
http://dx.doi.org/10.1155/2002/452527
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author Fabricius, Eva‐Maria
Gurr, Ulrike
Wildner, Gustav‐Paul
author_facet Fabricius, Eva‐Maria
Gurr, Ulrike
Wildner, Gustav‐Paul
author_sort Fabricius, Eva‐Maria
collection PubMed
description The survival of patients with a head‐and‐neck squamous cell carcinoma is determined by loco‐regional recurrence and second primary carcinomas. As a complement to histopathology, molecular changes of tumour marginal and tumour distant tissue may confirm curative surgical tumour extirpation. We tested telomerase activity with PCR‐ELISA kits. 20 tumour margin biopsies were chosen by the surgeon from 20 patients. In addition, 3 tissue samples were taken from each of 20 additional patients, one from the carcinoma centre, the tumour margin and one distant from the tumour. 50% of the carcinoma centres were telomerase‐positive. Thirteen of the 40 tumour margin samples showed increased telomerase levels, and in 3 of these residual carcinoma was histopathologically detected. Six of the 20 tumour distant tissues revealed increased telomerase levels. Telomerase positivity in carcinoma‐free tumour margins correlated with a good prognosis. Confirmation of the results in a larger patient group is needed.
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spelling pubmed-46182242016-01-12 Telomerase Activity Levels in the Surgical Margin and Tumour Distant Tissue of the Squamous Cell Carcinoma of the Head-And-Neck Fabricius, Eva‐Maria Gurr, Ulrike Wildner, Gustav‐Paul Anal Cell Pathol Other The survival of patients with a head‐and‐neck squamous cell carcinoma is determined by loco‐regional recurrence and second primary carcinomas. As a complement to histopathology, molecular changes of tumour marginal and tumour distant tissue may confirm curative surgical tumour extirpation. We tested telomerase activity with PCR‐ELISA kits. 20 tumour margin biopsies were chosen by the surgeon from 20 patients. In addition, 3 tissue samples were taken from each of 20 additional patients, one from the carcinoma centre, the tumour margin and one distant from the tumour. 50% of the carcinoma centres were telomerase‐positive. Thirteen of the 40 tumour margin samples showed increased telomerase levels, and in 3 of these residual carcinoma was histopathologically detected. Six of the 20 tumour distant tissues revealed increased telomerase levels. Telomerase positivity in carcinoma‐free tumour margins correlated with a good prognosis. Confirmation of the results in a larger patient group is needed. IOS Press 2002 2002-01-01 /pmc/articles/PMC4618224/ /pubmed/12122281 http://dx.doi.org/10.1155/2002/452527 Text en Copyright © 2002 Hindawi Publishing Corporation.
spellingShingle Other
Fabricius, Eva‐Maria
Gurr, Ulrike
Wildner, Gustav‐Paul
Telomerase Activity Levels in the Surgical Margin and Tumour Distant Tissue of the Squamous Cell Carcinoma of the Head-And-Neck
title Telomerase Activity Levels in the Surgical Margin and Tumour Distant Tissue of the Squamous Cell Carcinoma of the Head-And-Neck
title_full Telomerase Activity Levels in the Surgical Margin and Tumour Distant Tissue of the Squamous Cell Carcinoma of the Head-And-Neck
title_fullStr Telomerase Activity Levels in the Surgical Margin and Tumour Distant Tissue of the Squamous Cell Carcinoma of the Head-And-Neck
title_full_unstemmed Telomerase Activity Levels in the Surgical Margin and Tumour Distant Tissue of the Squamous Cell Carcinoma of the Head-And-Neck
title_short Telomerase Activity Levels in the Surgical Margin and Tumour Distant Tissue of the Squamous Cell Carcinoma of the Head-And-Neck
title_sort telomerase activity levels in the surgical margin and tumour distant tissue of the squamous cell carcinoma of the head-and-neck
topic Other
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618224/
https://www.ncbi.nlm.nih.gov/pubmed/12122281
http://dx.doi.org/10.1155/2002/452527
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