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Susceptibility of Snails to Infection with Schistosomes is influenced by Temperature and Expression of Heat Shock Proteins

The freshwater snail, Biomphalaria glabrata is the obligate intermediate host for the transmission of the parasitic trematode, Schistosoma mansoni the causative agent of the chronic debilitating neglected tropical disease, schistosomiasis. We showed previously that in juvenile snails, early and sign...

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Autores principales: Knight, Matty, Elhelu, O, Smith, M, Haugen, B, Miller, A, Raghavan, N, Wellman, C, Cousin, C, Dixon, F, Mann, V, Rinaldi, G, Ittiprasert, W, Brindley, PJ
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618387/
https://www.ncbi.nlm.nih.gov/pubmed/26504668
http://dx.doi.org/10.4172/2161-1165.1000189
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author Knight, Matty
Elhelu, O
Smith, M
Haugen, B
Miller, A
Raghavan, N
Wellman, C
Cousin, C
Dixon, F
Mann, V
Rinaldi, G
Ittiprasert, W
Brindley, PJ
author_facet Knight, Matty
Elhelu, O
Smith, M
Haugen, B
Miller, A
Raghavan, N
Wellman, C
Cousin, C
Dixon, F
Mann, V
Rinaldi, G
Ittiprasert, W
Brindley, PJ
author_sort Knight, Matty
collection PubMed
description The freshwater snail, Biomphalaria glabrata is the obligate intermediate host for the transmission of the parasitic trematode, Schistosoma mansoni the causative agent of the chronic debilitating neglected tropical disease, schistosomiasis. We showed previously that in juvenile snails, early and significant induction of stress manifested by the expression of stress proteins, Hsp 70, Hsp 90 and reverse transcriptase (RT) of the non- LTR retrotransposon, nimbus, is a characteristic feature of juvenile susceptible NMRI but not resistant BS-90 snails. These latter, however, could be rendered susceptible after mild heat shock at 32°C, revealing that resistance in the BS-90 resistant snail to schistosomes is a temperature dependent trait. Here we tested the hypothesis that maintenance of BS-90 resistant snails at the permissive temperature for several generations affects the resistance phenotype displayed at the non-permissive temperature of 25°C. The progeny of BS-90 snails bred and maintained through several generations (F1 to F4) at 32°C were susceptible to the schistosome infection when returned to room temperature, shedding cercariae at four weeks post-infection. Moreover, the study of expression levels of the heat shock protein (Hsp) 70 protein by ELISA and western blot analysis, showed that this protein is also differentially expressed between susceptible and resistant snails, with susceptible snails expressing more protein than their resistant counterparts after early exposure to wild-type but not to radiation-attenuated miracidia. These data suggested that in the face of global warming, the ability to sustain a reduction in schistosomiasis by using refractory snails as a strategy to block transmission of the disease might prove challenging since non-lethal elevation in temperature, affects snail susceptibility to S. mansoni.
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spelling pubmed-46183872015-10-24 Susceptibility of Snails to Infection with Schistosomes is influenced by Temperature and Expression of Heat Shock Proteins Knight, Matty Elhelu, O Smith, M Haugen, B Miller, A Raghavan, N Wellman, C Cousin, C Dixon, F Mann, V Rinaldi, G Ittiprasert, W Brindley, PJ Epidemiology (Sunnyvale) Article The freshwater snail, Biomphalaria glabrata is the obligate intermediate host for the transmission of the parasitic trematode, Schistosoma mansoni the causative agent of the chronic debilitating neglected tropical disease, schistosomiasis. We showed previously that in juvenile snails, early and significant induction of stress manifested by the expression of stress proteins, Hsp 70, Hsp 90 and reverse transcriptase (RT) of the non- LTR retrotransposon, nimbus, is a characteristic feature of juvenile susceptible NMRI but not resistant BS-90 snails. These latter, however, could be rendered susceptible after mild heat shock at 32°C, revealing that resistance in the BS-90 resistant snail to schistosomes is a temperature dependent trait. Here we tested the hypothesis that maintenance of BS-90 resistant snails at the permissive temperature for several generations affects the resistance phenotype displayed at the non-permissive temperature of 25°C. The progeny of BS-90 snails bred and maintained through several generations (F1 to F4) at 32°C were susceptible to the schistosome infection when returned to room temperature, shedding cercariae at four weeks post-infection. Moreover, the study of expression levels of the heat shock protein (Hsp) 70 protein by ELISA and western blot analysis, showed that this protein is also differentially expressed between susceptible and resistant snails, with susceptible snails expressing more protein than their resistant counterparts after early exposure to wild-type but not to radiation-attenuated miracidia. These data suggested that in the face of global warming, the ability to sustain a reduction in schistosomiasis by using refractory snails as a strategy to block transmission of the disease might prove challenging since non-lethal elevation in temperature, affects snail susceptibility to S. mansoni. 2015-06-21 2015-06 /pmc/articles/PMC4618387/ /pubmed/26504668 http://dx.doi.org/10.4172/2161-1165.1000189 Text en http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Knight, Matty
Elhelu, O
Smith, M
Haugen, B
Miller, A
Raghavan, N
Wellman, C
Cousin, C
Dixon, F
Mann, V
Rinaldi, G
Ittiprasert, W
Brindley, PJ
Susceptibility of Snails to Infection with Schistosomes is influenced by Temperature and Expression of Heat Shock Proteins
title Susceptibility of Snails to Infection with Schistosomes is influenced by Temperature and Expression of Heat Shock Proteins
title_full Susceptibility of Snails to Infection with Schistosomes is influenced by Temperature and Expression of Heat Shock Proteins
title_fullStr Susceptibility of Snails to Infection with Schistosomes is influenced by Temperature and Expression of Heat Shock Proteins
title_full_unstemmed Susceptibility of Snails to Infection with Schistosomes is influenced by Temperature and Expression of Heat Shock Proteins
title_short Susceptibility of Snails to Infection with Schistosomes is influenced by Temperature and Expression of Heat Shock Proteins
title_sort susceptibility of snails to infection with schistosomes is influenced by temperature and expression of heat shock proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618387/
https://www.ncbi.nlm.nih.gov/pubmed/26504668
http://dx.doi.org/10.4172/2161-1165.1000189
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