ChEC-seq kinetics discriminates transcription factor binding sites by DNA sequence and shape in vivo

Chromatin endogenous cleavage (ChEC) uses fusion of a protein of interest to micrococcal nuclease (MNase) to target calcium-dependent cleavage to specific genomic loci in vivo. Here we report the combination of ChEC with high-throughput sequencing (ChEC-seq) to map budding yeast transcription factor...

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Detalles Bibliográficos
Autores principales: Zentner, Gabriel E., Kasinathan, Sivakanthan, Xin, Beibei, Rohs, Remo, Henikoff, Steven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618392/
https://www.ncbi.nlm.nih.gov/pubmed/26490019
http://dx.doi.org/10.1038/ncomms9733

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618392/
https://www.ncbi.nlm.nih.gov/pubmed/26490019
http://dx.doi.org/10.1038/ncomms9733

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