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Technique and Feasibility of a Dual Staining Method for Estrogen Receptors and AgNORs

A new staining method for dual demonstration of Estrogen receptors (ER) and argyrophilc Nucleolus‐Organizer Regions (AgNORs) was developed. To rule out possible reciprocal effects, serial slides of 10 invasive ductale breast cancers were stained with either the single staining method or the simultan...

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Autores principales: Günther, Lukas, Hufnagl, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618433/
https://www.ncbi.nlm.nih.gov/pubmed/11205317
http://dx.doi.org/10.1155/2000/565976
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author Günther, Lukas
Hufnagl, Peter
author_facet Günther, Lukas
Hufnagl, Peter
author_sort Günther, Lukas
collection PubMed
description A new staining method for dual demonstration of Estrogen receptors (ER) and argyrophilc Nucleolus‐Organizer Regions (AgNORs) was developed. To rule out possible reciprocal effects, serial slides of 10 invasive ductale breast cancers were stained with either the single staining method or the simultaneous ER/AgNOR‐staining method and investigated comparatively. By measuring the slides with the image analysis system AMBA, reciprocal effects could be excluded. It was proven that dual staining of both markers results in a reproducible and specific staining result. We concluded that it is justified to measure AgNORs in immunohistochemically stained cells.
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spelling pubmed-46184332016-01-12 Technique and Feasibility of a Dual Staining Method for Estrogen Receptors and AgNORs Günther, Lukas Hufnagl, Peter Anal Cell Pathol Technical Note A new staining method for dual demonstration of Estrogen receptors (ER) and argyrophilc Nucleolus‐Organizer Regions (AgNORs) was developed. To rule out possible reciprocal effects, serial slides of 10 invasive ductale breast cancers were stained with either the single staining method or the simultaneous ER/AgNOR‐staining method and investigated comparatively. By measuring the slides with the image analysis system AMBA, reciprocal effects could be excluded. It was proven that dual staining of both markers results in a reproducible and specific staining result. We concluded that it is justified to measure AgNORs in immunohistochemically stained cells. IOS Press 2000 2000-01-01 /pmc/articles/PMC4618433/ /pubmed/11205317 http://dx.doi.org/10.1155/2000/565976 Text en Copyright © 2000 Hindawi Publishing Corporation.
spellingShingle Technical Note
Günther, Lukas
Hufnagl, Peter
Technique and Feasibility of a Dual Staining Method for Estrogen Receptors and AgNORs
title Technique and Feasibility of a Dual Staining Method for Estrogen Receptors and AgNORs
title_full Technique and Feasibility of a Dual Staining Method for Estrogen Receptors and AgNORs
title_fullStr Technique and Feasibility of a Dual Staining Method for Estrogen Receptors and AgNORs
title_full_unstemmed Technique and Feasibility of a Dual Staining Method for Estrogen Receptors and AgNORs
title_short Technique and Feasibility of a Dual Staining Method for Estrogen Receptors and AgNORs
title_sort technique and feasibility of a dual staining method for estrogen receptors and agnors
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618433/
https://www.ncbi.nlm.nih.gov/pubmed/11205317
http://dx.doi.org/10.1155/2000/565976
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