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Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9

Mouse spermatogonial stem cells (SSCs) can be cultured for multiplication and maintained for long periods while preserving their spermatogenic ability. Although the cultured SSCs, named germline stem (GS) cells, are targets of genome modification, this process remains technically difficult. In the p...

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Detalles Bibliográficos
Autores principales:	Sato, Takuya, Sakuma, Tetsushi, Yokonishi, Tetsuhiro, Katagiri, Kumiko, Kamimura, Satoshi, Ogonuki, Narumi, Ogura, Atsuo, Yamamoto, Takashi, Ogawa, Takehiko
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2015
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618438/ https://www.ncbi.nlm.nih.gov/pubmed/26095606 http://dx.doi.org/10.1016/j.stemcr.2015.05.011

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