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In Vitro Model for Studying Malignancy Associated Changes
Malignancy associated changes (MAC) can be defined as subtle morphological and physiologic changes that are found in ostensibly normal cells of patients harboring malignant disease. It has been postulated that MAC have a potential to become a useful tool in detection, diagnosis and prognosis of mali...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
IOS Press
2003
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618911/ https://www.ncbi.nlm.nih.gov/pubmed/12632019 http://dx.doi.org/10.1155/2003/238921 |
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author | Sun, Xiao Rong Zheng, Yonghong MacAulay, Calum Lam, Stephen Doudkine, Alexei Palcic, Branko |
author_facet | Sun, Xiao Rong Zheng, Yonghong MacAulay, Calum Lam, Stephen Doudkine, Alexei Palcic, Branko |
author_sort | Sun, Xiao Rong |
collection | PubMed |
description | Malignancy associated changes (MAC) can be defined as subtle morphological and physiologic changes that are found in ostensibly normal cells of patients harboring malignant disease. It has been postulated that MAC have a potential to become a useful tool in detection, diagnosis and prognosis of malignant diseases. An in vitro cell culture model system was designed to study interactions between non‐small cell lung cancer (NSCLC) and the normal bronchial epithelium of the human respiratory tract in vivo to see if the MAC‐like phenomenon can be detected in such a system. In this study we examined changes in nuclear features of normal human bronchial epithelial cells (NHBE) when they were co‐cultured with cells derived from a lung cancer cell line NCI‐H460. Using discriminant function analysis, nuclear features were determined which allow maximal discrimination between normal cells incubated with or without cancerous cells. Our results demonstrate that MAC appear to be specific to changes induced by malignancy, and that these changes differ from those induced by growth factors in the serum. This study provides evidence in support to the hypothesis that MAC are induced by a soluble factor(s) released by malignant cells. Colour figure can be viewed on http://www.esacp.org/acp/2003/25‐2/sun.htm. |
format | Online Article Text |
id | pubmed-4618911 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | IOS Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-46189112016-01-12 In Vitro Model for Studying Malignancy Associated Changes Sun, Xiao Rong Zheng, Yonghong MacAulay, Calum Lam, Stephen Doudkine, Alexei Palcic, Branko Anal Cell Pathol Other Malignancy associated changes (MAC) can be defined as subtle morphological and physiologic changes that are found in ostensibly normal cells of patients harboring malignant disease. It has been postulated that MAC have a potential to become a useful tool in detection, diagnosis and prognosis of malignant diseases. An in vitro cell culture model system was designed to study interactions between non‐small cell lung cancer (NSCLC) and the normal bronchial epithelium of the human respiratory tract in vivo to see if the MAC‐like phenomenon can be detected in such a system. In this study we examined changes in nuclear features of normal human bronchial epithelial cells (NHBE) when they were co‐cultured with cells derived from a lung cancer cell line NCI‐H460. Using discriminant function analysis, nuclear features were determined which allow maximal discrimination between normal cells incubated with or without cancerous cells. Our results demonstrate that MAC appear to be specific to changes induced by malignancy, and that these changes differ from those induced by growth factors in the serum. This study provides evidence in support to the hypothesis that MAC are induced by a soluble factor(s) released by malignant cells. Colour figure can be viewed on http://www.esacp.org/acp/2003/25‐2/sun.htm. IOS Press 2003 2003-01-01 /pmc/articles/PMC4618911/ /pubmed/12632019 http://dx.doi.org/10.1155/2003/238921 Text en Copyright © 2003 Hindawi Publishing Corporation. |
spellingShingle | Other Sun, Xiao Rong Zheng, Yonghong MacAulay, Calum Lam, Stephen Doudkine, Alexei Palcic, Branko In Vitro Model for Studying Malignancy Associated Changes |
title |
In Vitro Model for Studying Malignancy Associated Changes |
title_full |
In Vitro Model for Studying Malignancy Associated Changes |
title_fullStr |
In Vitro Model for Studying Malignancy Associated Changes |
title_full_unstemmed |
In Vitro Model for Studying Malignancy Associated Changes |
title_short |
In Vitro Model for Studying Malignancy Associated Changes |
title_sort | in vitro model for studying malignancy associated changes |
topic | Other |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618911/ https://www.ncbi.nlm.nih.gov/pubmed/12632019 http://dx.doi.org/10.1155/2003/238921 |
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