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In Vitro Model for Studying Malignancy Associated Changes

Malignancy associated changes (MAC) can be defined as subtle morphological and physiologic changes that are found in ostensibly normal cells of patients harboring malignant disease. It has been postulated that MAC have a potential to become a useful tool in detection, diagnosis and prognosis of mali...

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Autores principales: Sun, Xiao Rong, Zheng, Yonghong, MacAulay, Calum, Lam, Stephen, Doudkine, Alexei, Palcic, Branko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618911/
https://www.ncbi.nlm.nih.gov/pubmed/12632019
http://dx.doi.org/10.1155/2003/238921
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author Sun, Xiao Rong
Zheng, Yonghong
MacAulay, Calum
Lam, Stephen
Doudkine, Alexei
Palcic, Branko
author_facet Sun, Xiao Rong
Zheng, Yonghong
MacAulay, Calum
Lam, Stephen
Doudkine, Alexei
Palcic, Branko
author_sort Sun, Xiao Rong
collection PubMed
description Malignancy associated changes (MAC) can be defined as subtle morphological and physiologic changes that are found in ostensibly normal cells of patients harboring malignant disease. It has been postulated that MAC have a potential to become a useful tool in detection, diagnosis and prognosis of malignant diseases. An in vitro cell culture model system was designed to study interactions between non‐small cell lung cancer (NSCLC) and the normal bronchial epithelium of the human respiratory tract in vivo to see if the MAC‐like phenomenon can be detected in such a system. In this study we examined changes in nuclear features of normal human bronchial epithelial cells (NHBE) when they were co‐cultured with cells derived from a lung cancer cell line NCI‐H460. Using discriminant function analysis, nuclear features were determined which allow maximal discrimination between normal cells incubated with or without cancerous cells. Our results demonstrate that MAC appear to be specific to changes induced by malignancy, and that these changes differ from those induced by growth factors in the serum. This study provides evidence in support to the hypothesis that MAC are induced by a soluble factor(s) released by malignant cells. Colour figure can be viewed on http://www.esacp.org/acp/2003/25‐2/sun.htm.
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spelling pubmed-46189112016-01-12 In Vitro Model for Studying Malignancy Associated Changes Sun, Xiao Rong Zheng, Yonghong MacAulay, Calum Lam, Stephen Doudkine, Alexei Palcic, Branko Anal Cell Pathol Other Malignancy associated changes (MAC) can be defined as subtle morphological and physiologic changes that are found in ostensibly normal cells of patients harboring malignant disease. It has been postulated that MAC have a potential to become a useful tool in detection, diagnosis and prognosis of malignant diseases. An in vitro cell culture model system was designed to study interactions between non‐small cell lung cancer (NSCLC) and the normal bronchial epithelium of the human respiratory tract in vivo to see if the MAC‐like phenomenon can be detected in such a system. In this study we examined changes in nuclear features of normal human bronchial epithelial cells (NHBE) when they were co‐cultured with cells derived from a lung cancer cell line NCI‐H460. Using discriminant function analysis, nuclear features were determined which allow maximal discrimination between normal cells incubated with or without cancerous cells. Our results demonstrate that MAC appear to be specific to changes induced by malignancy, and that these changes differ from those induced by growth factors in the serum. This study provides evidence in support to the hypothesis that MAC are induced by a soluble factor(s) released by malignant cells. Colour figure can be viewed on http://www.esacp.org/acp/2003/25‐2/sun.htm. IOS Press 2003 2003-01-01 /pmc/articles/PMC4618911/ /pubmed/12632019 http://dx.doi.org/10.1155/2003/238921 Text en Copyright © 2003 Hindawi Publishing Corporation.
spellingShingle Other
Sun, Xiao Rong
Zheng, Yonghong
MacAulay, Calum
Lam, Stephen
Doudkine, Alexei
Palcic, Branko
In Vitro Model for Studying Malignancy Associated Changes
title In Vitro Model for Studying Malignancy Associated Changes
title_full In Vitro Model for Studying Malignancy Associated Changes
title_fullStr In Vitro Model for Studying Malignancy Associated Changes
title_full_unstemmed In Vitro Model for Studying Malignancy Associated Changes
title_short In Vitro Model for Studying Malignancy Associated Changes
title_sort in vitro model for studying malignancy associated changes
topic Other
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618911/
https://www.ncbi.nlm.nih.gov/pubmed/12632019
http://dx.doi.org/10.1155/2003/238921
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