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Mansonella, including a Potential New Species, as Common Parasites in Children in Gabon

BACKGROUND: Like other tropical African countries, Gabon is afflicted by many parasitic diseases, including filariases such as loiasis and mansonellosis. This study aimed to assess the prevalence of these two filarial diseases in febrile and afebrile children using quantitative real-time PCR and sta...

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Autores principales: Mourembou, Gaël, Fenollar, Florence, Lekana-Douki, Jean Bernard, Ndjoyi Mbiguino, Angelique, Maghendji Nzondo, Sydney, Matsiegui, Pierre Blaise, Zoleko Manego, Rella, Ehounoud, Cyrille Herve Bile, Bittar, Fadi, Raoult, Didier, Mediannikov, Oleg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618925/
https://www.ncbi.nlm.nih.gov/pubmed/26484866
http://dx.doi.org/10.1371/journal.pntd.0004155
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author Mourembou, Gaël
Fenollar, Florence
Lekana-Douki, Jean Bernard
Ndjoyi Mbiguino, Angelique
Maghendji Nzondo, Sydney
Matsiegui, Pierre Blaise
Zoleko Manego, Rella
Ehounoud, Cyrille Herve Bile
Bittar, Fadi
Raoult, Didier
Mediannikov, Oleg
author_facet Mourembou, Gaël
Fenollar, Florence
Lekana-Douki, Jean Bernard
Ndjoyi Mbiguino, Angelique
Maghendji Nzondo, Sydney
Matsiegui, Pierre Blaise
Zoleko Manego, Rella
Ehounoud, Cyrille Herve Bile
Bittar, Fadi
Raoult, Didier
Mediannikov, Oleg
author_sort Mourembou, Gaël
collection PubMed
description BACKGROUND: Like other tropical African countries, Gabon is afflicted by many parasitic diseases, including filariases such as loiasis and mansonellosis. This study aimed to assess the prevalence of these two filarial diseases in febrile and afebrile children using quantitative real-time PCR and standard PCR assays coupled with sequencing. METHODOLOGY/PRINCIPAL FINDINGS: DNA from blood specimens of 1,418 Gabonese children (1,258 febrile and 160 afebrile) were analyzed. Overall, filarial DNA was detected in 95 (6.7%) children, including 67 positive for M. perstans (4.7%), which was the most common. M. perstans was detected in 61/1,258 febrile children (4.8%) and 6/160 afebrile children (3.8%, P = 0.6). Its prevalence increased statistically with age: 3.5%, 7.7% and 10.6% in children aged ≤5, 6–10 and 11–15 years, respectively. M. perstans prevalence was significantly higher in Koulamoutou and Lastourville (12% and 10.5%, respectively) than in Franceville and Fougamou (2.6% and 2.4%, respectively). Loa loa was detected in seven febrile children including one co-infection with M. perstans. Finally, 21 filarial DNA positive were negative for M. perstans and Loa loa, but ITS sequencing could be performed for 12 and allowed the identification of a potential new species of Mansonella provisionally called “DEUX”. Mansonella sp. “DEUX” was detected only in febrile children. CONCLUSIONS/SIGNIFICANCE: Further study should be performed to characterize Mansonella sp. “DEUX” and evaluate the clinical significance of mansonellosis in humans.
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spelling pubmed-46189252015-10-29 Mansonella, including a Potential New Species, as Common Parasites in Children in Gabon Mourembou, Gaël Fenollar, Florence Lekana-Douki, Jean Bernard Ndjoyi Mbiguino, Angelique Maghendji Nzondo, Sydney Matsiegui, Pierre Blaise Zoleko Manego, Rella Ehounoud, Cyrille Herve Bile Bittar, Fadi Raoult, Didier Mediannikov, Oleg PLoS Negl Trop Dis Research Article BACKGROUND: Like other tropical African countries, Gabon is afflicted by many parasitic diseases, including filariases such as loiasis and mansonellosis. This study aimed to assess the prevalence of these two filarial diseases in febrile and afebrile children using quantitative real-time PCR and standard PCR assays coupled with sequencing. METHODOLOGY/PRINCIPAL FINDINGS: DNA from blood specimens of 1,418 Gabonese children (1,258 febrile and 160 afebrile) were analyzed. Overall, filarial DNA was detected in 95 (6.7%) children, including 67 positive for M. perstans (4.7%), which was the most common. M. perstans was detected in 61/1,258 febrile children (4.8%) and 6/160 afebrile children (3.8%, P = 0.6). Its prevalence increased statistically with age: 3.5%, 7.7% and 10.6% in children aged ≤5, 6–10 and 11–15 years, respectively. M. perstans prevalence was significantly higher in Koulamoutou and Lastourville (12% and 10.5%, respectively) than in Franceville and Fougamou (2.6% and 2.4%, respectively). Loa loa was detected in seven febrile children including one co-infection with M. perstans. Finally, 21 filarial DNA positive were negative for M. perstans and Loa loa, but ITS sequencing could be performed for 12 and allowed the identification of a potential new species of Mansonella provisionally called “DEUX”. Mansonella sp. “DEUX” was detected only in febrile children. CONCLUSIONS/SIGNIFICANCE: Further study should be performed to characterize Mansonella sp. “DEUX” and evaluate the clinical significance of mansonellosis in humans. Public Library of Science 2015-10-20 /pmc/articles/PMC4618925/ /pubmed/26484866 http://dx.doi.org/10.1371/journal.pntd.0004155 Text en © 2015 Mourembou et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mourembou, Gaël
Fenollar, Florence
Lekana-Douki, Jean Bernard
Ndjoyi Mbiguino, Angelique
Maghendji Nzondo, Sydney
Matsiegui, Pierre Blaise
Zoleko Manego, Rella
Ehounoud, Cyrille Herve Bile
Bittar, Fadi
Raoult, Didier
Mediannikov, Oleg
Mansonella, including a Potential New Species, as Common Parasites in Children in Gabon
title Mansonella, including a Potential New Species, as Common Parasites in Children in Gabon
title_full Mansonella, including a Potential New Species, as Common Parasites in Children in Gabon
title_fullStr Mansonella, including a Potential New Species, as Common Parasites in Children in Gabon
title_full_unstemmed Mansonella, including a Potential New Species, as Common Parasites in Children in Gabon
title_short Mansonella, including a Potential New Species, as Common Parasites in Children in Gabon
title_sort mansonella, including a potential new species, as common parasites in children in gabon
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618925/
https://www.ncbi.nlm.nih.gov/pubmed/26484866
http://dx.doi.org/10.1371/journal.pntd.0004155
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