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DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs

BACKGROUND: CRISPR genome-editing technology makes it possible to quickly and cheaply delete non-protein-coding regulatory elements. We present a vector system adapted for this purpose called DECKO (Double Excision CRISPR Knockout), which applies a simple two-step cloning to generate lentiviral vect...

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Detalles Bibliográficos
Autores principales:	Aparicio-Prat, Estel, Arnan, Carme, Sala, Ilaria, Bosch, Núria, Guigó, Roderic, Johnson, Rory
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619085/ https://www.ncbi.nlm.nih.gov/pubmed/26493208 http://dx.doi.org/10.1186/s12864-015-2086-z

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