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Oral Cancer Genesis and Progression: DNA Near-Diploid Aneuploidization and Endoreduplication by High Resolution Flow Cytometry

Oral potentially malignant lesions (OPMLs) with dysplasia and aneuploidy are thought to have a high risk of progression into oral squamous cell carcinomas (OSCCs). Non-dysplastic “oral distant fields” (ODFs), characterized by clinically normal appearing mucosa sited at a distance from co-existing OP...

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Autores principales: Donadini, Alessandra, Maffei, Massimo, Cavallero, Antonio, Pentenero, Monica, Malacarne, Davide, Nallo, Emanuela Di, Truini, Mauro, Navone, Roberto, Mereu, Paola, Scala, Marco, Santelli, Alida, Gandolfo, Sergio, Giaretti, Walter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619238/
https://www.ncbi.nlm.nih.gov/pubmed/20448331
http://dx.doi.org/10.3233/CLO-2010-0525
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author Donadini, Alessandra
Maffei, Massimo
Cavallero, Antonio
Pentenero, Monica
Malacarne, Davide
Nallo, Emanuela Di
Truini, Mauro
Navone, Roberto
Mereu, Paola
Scala, Marco
Santelli, Alida
Gandolfo, Sergio
Giaretti, Walter
author_facet Donadini, Alessandra
Maffei, Massimo
Cavallero, Antonio
Pentenero, Monica
Malacarne, Davide
Nallo, Emanuela Di
Truini, Mauro
Navone, Roberto
Mereu, Paola
Scala, Marco
Santelli, Alida
Gandolfo, Sergio
Giaretti, Walter
author_sort Donadini, Alessandra
collection PubMed
description Oral potentially malignant lesions (OPMLs) with dysplasia and aneuploidy are thought to have a high risk of progression into oral squamous cell carcinomas (OSCCs). Non-dysplastic “oral distant fields” (ODFs), characterized by clinically normal appearing mucosa sited at a distance from co-existing OPMLs, and non-dysplastic OPMLs may also represent an early pre-cancerous state. ODFs, OPMLs without and with dysplasia and OSCCs were investigated by high resolution DNA content flow cytometry (FCM). ODFs and OPMLs without dysplasia were DNA aneuploid respectively in 7/82 (8.5%) and 25/109 (23%) cases. “True normal oral mucosa” and human lymphocytes from healthy donors were DNA diploid in all cases and were used as sex specific DNA diploid controls. Dysplastic OPMLs and OSCCs were DNA aneuploid in 12/26 (46%) and 12/13 (92%) cases. The DNA aneuploid sublines were characterized by the DNA Index (DI ≠ 1). Aneuploid sublines in ODFs and in non-dysplastic and dysplastic OPMLs were near-diploid (DI < 1.4) respectively in all, 2/3 and 1/3 of the cases. DNA aneuploid OSCCs, instead, were characterized prevalently by multiple aneuploid sublines (67%), which were commonly (57%) high-aneuploid (DI ≥ 1.4). DNA near-diploid aneuploid sublines in ODFs and OPMLs appear as early events of the oral carcinogenesis in agreement with the concept of field effect. Near-diploid aneuploidization is likely to reflect mechanisms of loss of symmetry in the chromosome mitotic division. High DNA aneuploid and multiple sublines in OPMLs with dysplasia and OSCCs suggest, instead, mechanisms of “endoreduplication” of diploid and near-diploid aneuploid cells and chromosomal loss. High resolution DNA FCM seems to enable the separation of subsequent progression steps of the oral carcinogenesis.
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spelling pubmed-46192382016-01-12 Oral Cancer Genesis and Progression: DNA Near-Diploid Aneuploidization and Endoreduplication by High Resolution Flow Cytometry Donadini, Alessandra Maffei, Massimo Cavallero, Antonio Pentenero, Monica Malacarne, Davide Nallo, Emanuela Di Truini, Mauro Navone, Roberto Mereu, Paola Scala, Marco Santelli, Alida Gandolfo, Sergio Giaretti, Walter Cell Oncol Other Oral potentially malignant lesions (OPMLs) with dysplasia and aneuploidy are thought to have a high risk of progression into oral squamous cell carcinomas (OSCCs). Non-dysplastic “oral distant fields” (ODFs), characterized by clinically normal appearing mucosa sited at a distance from co-existing OPMLs, and non-dysplastic OPMLs may also represent an early pre-cancerous state. ODFs, OPMLs without and with dysplasia and OSCCs were investigated by high resolution DNA content flow cytometry (FCM). ODFs and OPMLs without dysplasia were DNA aneuploid respectively in 7/82 (8.5%) and 25/109 (23%) cases. “True normal oral mucosa” and human lymphocytes from healthy donors were DNA diploid in all cases and were used as sex specific DNA diploid controls. Dysplastic OPMLs and OSCCs were DNA aneuploid in 12/26 (46%) and 12/13 (92%) cases. The DNA aneuploid sublines were characterized by the DNA Index (DI ≠ 1). Aneuploid sublines in ODFs and in non-dysplastic and dysplastic OPMLs were near-diploid (DI < 1.4) respectively in all, 2/3 and 1/3 of the cases. DNA aneuploid OSCCs, instead, were characterized prevalently by multiple aneuploid sublines (67%), which were commonly (57%) high-aneuploid (DI ≥ 1.4). DNA near-diploid aneuploid sublines in ODFs and OPMLs appear as early events of the oral carcinogenesis in agreement with the concept of field effect. Near-diploid aneuploidization is likely to reflect mechanisms of loss of symmetry in the chromosome mitotic division. High DNA aneuploid and multiple sublines in OPMLs with dysplasia and OSCCs suggest, instead, mechanisms of “endoreduplication” of diploid and near-diploid aneuploid cells and chromosomal loss. High resolution DNA FCM seems to enable the separation of subsequent progression steps of the oral carcinogenesis. IOS Press 2010 2010-05-06 /pmc/articles/PMC4619238/ /pubmed/20448331 http://dx.doi.org/10.3233/CLO-2010-0525 Text en Copyright © 2010 Hindawi Publishing Corporation and the authors.
spellingShingle Other
Donadini, Alessandra
Maffei, Massimo
Cavallero, Antonio
Pentenero, Monica
Malacarne, Davide
Nallo, Emanuela Di
Truini, Mauro
Navone, Roberto
Mereu, Paola
Scala, Marco
Santelli, Alida
Gandolfo, Sergio
Giaretti, Walter
Oral Cancer Genesis and Progression: DNA Near-Diploid Aneuploidization and Endoreduplication by High Resolution Flow Cytometry
title Oral Cancer Genesis and Progression: DNA Near-Diploid Aneuploidization and Endoreduplication by High Resolution Flow Cytometry
title_full Oral Cancer Genesis and Progression: DNA Near-Diploid Aneuploidization and Endoreduplication by High Resolution Flow Cytometry
title_fullStr Oral Cancer Genesis and Progression: DNA Near-Diploid Aneuploidization and Endoreduplication by High Resolution Flow Cytometry
title_full_unstemmed Oral Cancer Genesis and Progression: DNA Near-Diploid Aneuploidization and Endoreduplication by High Resolution Flow Cytometry
title_short Oral Cancer Genesis and Progression: DNA Near-Diploid Aneuploidization and Endoreduplication by High Resolution Flow Cytometry
title_sort oral cancer genesis and progression: dna near-diploid aneuploidization and endoreduplication by high resolution flow cytometry
topic Other
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619238/
https://www.ncbi.nlm.nih.gov/pubmed/20448331
http://dx.doi.org/10.3233/CLO-2010-0525
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