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Detection of the HA-33 protein in botulinum neurotoxin type G complex by mass spectrometry

BACKGROUND: The disease botulism is caused by intoxication with botulinum neurotoxins (BoNTs), extremely toxic proteins which cause paralysis. This neurotoxin is produced by some members of the Clostridium botulinum and closely related species, and is produced as a protein complex consisting of the...

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Autores principales: Kalb, Suzanne R., Baudys, Jakub, Barr, John R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619279/
https://www.ncbi.nlm.nih.gov/pubmed/26494251
http://dx.doi.org/10.1186/s12866-015-0567-5
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author Kalb, Suzanne R.
Baudys, Jakub
Barr, John R.
author_facet Kalb, Suzanne R.
Baudys, Jakub
Barr, John R.
author_sort Kalb, Suzanne R.
collection PubMed
description BACKGROUND: The disease botulism is caused by intoxication with botulinum neurotoxins (BoNTs), extremely toxic proteins which cause paralysis. This neurotoxin is produced by some members of the Clostridium botulinum and closely related species, and is produced as a protein complex consisting of the neurotoxin and neurotoxin-associated proteins (NAPs). There are seven known serotypes of BoNT, A-G, and the composition of the NAPs can differ between these serotypes. It was previously published that the BoNT/G complex consisted of BoNT/G, nontoxic-nonhemagglutinin (NTNH), Hemagglutinin 70 (HA-70), and HA-17, but that HA-33, a component of the protein complex of other serotypes of BoNT, was not found. METHODS: Components of the BoNT/G complex were first separated by SDS-PAGE, and bands corresponding to components of the complex were digested and analyzed by LC-MS/MS. RESULTS: Gel bands were identified with sequence coverages of 91 % for BoNT/G, 91 % for NTNH, 89 % for HA-70, and 88 % for HA-17. Notably, one gel band was also clearly identified as HA-33 with 93 % sequence coverage. CONCLUSIONS: The BoNT/G complex consists of BoNT/G, NTNH, HA-70, HA-17, and HA-33. These proteins form the progenitor form of BoNT/G, similar to all other HA positive progenitor toxin complexes.
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spelling pubmed-46192792015-10-26 Detection of the HA-33 protein in botulinum neurotoxin type G complex by mass spectrometry Kalb, Suzanne R. Baudys, Jakub Barr, John R. BMC Microbiol Research Article BACKGROUND: The disease botulism is caused by intoxication with botulinum neurotoxins (BoNTs), extremely toxic proteins which cause paralysis. This neurotoxin is produced by some members of the Clostridium botulinum and closely related species, and is produced as a protein complex consisting of the neurotoxin and neurotoxin-associated proteins (NAPs). There are seven known serotypes of BoNT, A-G, and the composition of the NAPs can differ between these serotypes. It was previously published that the BoNT/G complex consisted of BoNT/G, nontoxic-nonhemagglutinin (NTNH), Hemagglutinin 70 (HA-70), and HA-17, but that HA-33, a component of the protein complex of other serotypes of BoNT, was not found. METHODS: Components of the BoNT/G complex were first separated by SDS-PAGE, and bands corresponding to components of the complex were digested and analyzed by LC-MS/MS. RESULTS: Gel bands were identified with sequence coverages of 91 % for BoNT/G, 91 % for NTNH, 89 % for HA-70, and 88 % for HA-17. Notably, one gel band was also clearly identified as HA-33 with 93 % sequence coverage. CONCLUSIONS: The BoNT/G complex consists of BoNT/G, NTNH, HA-70, HA-17, and HA-33. These proteins form the progenitor form of BoNT/G, similar to all other HA positive progenitor toxin complexes. BioMed Central 2015-10-23 /pmc/articles/PMC4619279/ /pubmed/26494251 http://dx.doi.org/10.1186/s12866-015-0567-5 Text en © Kalb et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Kalb, Suzanne R.
Baudys, Jakub
Barr, John R.
Detection of the HA-33 protein in botulinum neurotoxin type G complex by mass spectrometry
title Detection of the HA-33 protein in botulinum neurotoxin type G complex by mass spectrometry
title_full Detection of the HA-33 protein in botulinum neurotoxin type G complex by mass spectrometry
title_fullStr Detection of the HA-33 protein in botulinum neurotoxin type G complex by mass spectrometry
title_full_unstemmed Detection of the HA-33 protein in botulinum neurotoxin type G complex by mass spectrometry
title_short Detection of the HA-33 protein in botulinum neurotoxin type G complex by mass spectrometry
title_sort detection of the ha-33 protein in botulinum neurotoxin type g complex by mass spectrometry
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619279/
https://www.ncbi.nlm.nih.gov/pubmed/26494251
http://dx.doi.org/10.1186/s12866-015-0567-5
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