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NF-Protocadherin Regulates Retinal Ganglion Cell Axon Behaviour in the Developing Visual System

Cell adhesion molecules play a central role in mediating axonal tract development within the nascent nervous system. NF-protocadherin (NFPC), a member of the non-clustered protocadherin family, has been shown to regulate retinal ganglion cell (RGC) axon and dendrite initiation, as well as influencin...

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Autores principales: Leung, Louis C., Harris, William A., Holt, Christine E., Piper, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619323/
https://www.ncbi.nlm.nih.gov/pubmed/26489017
http://dx.doi.org/10.1371/journal.pone.0141290
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author Leung, Louis C.
Harris, William A.
Holt, Christine E.
Piper, Michael
author_facet Leung, Louis C.
Harris, William A.
Holt, Christine E.
Piper, Michael
author_sort Leung, Louis C.
collection PubMed
description Cell adhesion molecules play a central role in mediating axonal tract development within the nascent nervous system. NF-protocadherin (NFPC), a member of the non-clustered protocadherin family, has been shown to regulate retinal ganglion cell (RGC) axon and dendrite initiation, as well as influencing axonal navigation within the mid-optic tract. However, whether NFPC mediates RGC axonal behaviour at other positions within the optic pathway remains unclear. Here we report that NFPC plays an important role in RGC axonogenesis, but not in intraretinal guidance. Moreover, axons with reduced NFPC levels exhibit insensitivity to Netrin-1, an attractive guidance cue expressed at the optic nerve head. Netrin-1 induces rapid turnover of NFPC localized to RGC growth cones, suggesting that the regulation of NFPC protein levels may underlie Netrin-1-mediated entry of RGC axons into the optic nerve head. At the tectum, we further reveal a function for NFPC in controlling RGC axonal entry into the final target area. Collectively, our results expand our understanding of the role of NFPC in RGC guidance and illustrate that this adhesion molecule contributes to axon behaviour at multiple points in the optic pathway.
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spelling pubmed-46193232015-10-29 NF-Protocadherin Regulates Retinal Ganglion Cell Axon Behaviour in the Developing Visual System Leung, Louis C. Harris, William A. Holt, Christine E. Piper, Michael PLoS One Research Article Cell adhesion molecules play a central role in mediating axonal tract development within the nascent nervous system. NF-protocadherin (NFPC), a member of the non-clustered protocadherin family, has been shown to regulate retinal ganglion cell (RGC) axon and dendrite initiation, as well as influencing axonal navigation within the mid-optic tract. However, whether NFPC mediates RGC axonal behaviour at other positions within the optic pathway remains unclear. Here we report that NFPC plays an important role in RGC axonogenesis, but not in intraretinal guidance. Moreover, axons with reduced NFPC levels exhibit insensitivity to Netrin-1, an attractive guidance cue expressed at the optic nerve head. Netrin-1 induces rapid turnover of NFPC localized to RGC growth cones, suggesting that the regulation of NFPC protein levels may underlie Netrin-1-mediated entry of RGC axons into the optic nerve head. At the tectum, we further reveal a function for NFPC in controlling RGC axonal entry into the final target area. Collectively, our results expand our understanding of the role of NFPC in RGC guidance and illustrate that this adhesion molecule contributes to axon behaviour at multiple points in the optic pathway. Public Library of Science 2015-10-21 /pmc/articles/PMC4619323/ /pubmed/26489017 http://dx.doi.org/10.1371/journal.pone.0141290 Text en © 2015 Leung et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Leung, Louis C.
Harris, William A.
Holt, Christine E.
Piper, Michael
NF-Protocadherin Regulates Retinal Ganglion Cell Axon Behaviour in the Developing Visual System
title NF-Protocadherin Regulates Retinal Ganglion Cell Axon Behaviour in the Developing Visual System
title_full NF-Protocadherin Regulates Retinal Ganglion Cell Axon Behaviour in the Developing Visual System
title_fullStr NF-Protocadherin Regulates Retinal Ganglion Cell Axon Behaviour in the Developing Visual System
title_full_unstemmed NF-Protocadherin Regulates Retinal Ganglion Cell Axon Behaviour in the Developing Visual System
title_short NF-Protocadherin Regulates Retinal Ganglion Cell Axon Behaviour in the Developing Visual System
title_sort nf-protocadherin regulates retinal ganglion cell axon behaviour in the developing visual system
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619323/
https://www.ncbi.nlm.nih.gov/pubmed/26489017
http://dx.doi.org/10.1371/journal.pone.0141290
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