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Hepatitis D virus and hepatitis B virus infection in Amerindian communities of the Amazonas state, Colombia

BACKGROUND: In Colombia, cases of Hepatitis D virus (HDV) infection have been officially described since 1985 mainly in Amerindian population from Sierra Nevada de Santa Marta (North Caribbean Coast), Uraba (North West), and Amazon (South East). The last official report of a clinical case of HDV inf...

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Autores principales: di Filippo Villa, Diana, Cortes-Mancera, Fabian, Payares, Edra, Montes, Neyla, de la Hoz, Fernando, Arbelaez, Maria Patricia, Correa, Gonzalo, Navas, Maria-Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619413/
https://www.ncbi.nlm.nih.gov/pubmed/26497287
http://dx.doi.org/10.1186/s12985-015-0402-5
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author di Filippo Villa, Diana
Cortes-Mancera, Fabian
Payares, Edra
Montes, Neyla
de la Hoz, Fernando
Arbelaez, Maria Patricia
Correa, Gonzalo
Navas, Maria-Cristina
author_facet di Filippo Villa, Diana
Cortes-Mancera, Fabian
Payares, Edra
Montes, Neyla
de la Hoz, Fernando
Arbelaez, Maria Patricia
Correa, Gonzalo
Navas, Maria-Cristina
author_sort di Filippo Villa, Diana
collection PubMed
description BACKGROUND: In Colombia, cases of Hepatitis D virus (HDV) infection have been officially described since 1985 mainly in Amerindian population from Sierra Nevada de Santa Marta (North Caribbean Coast), Uraba (North West), and Amazon (South East). The last official report of a clinical case of HDV infection in Colombia was registered in 2005. OBJECTIVES: The aims of this study were to identify cases of HDV and/or Hepatitis B virus (HBV) infection in asymptomatic Amerindians from Amazonas state, South East Colombia, and to describe the circulating viral genotypes in this population. STUDY DESIGN: The study population was recruited in 19 Amerindian communities in the Amazonas state. Individuals over 18 years old were screened by rapid test for Hepatitis B surface Antigen (HBsAg). Blood samples obtained from individuals positives for HBsAg in the rapid-test assay were analyzed for HBsAg, anti-HBc, anti-HDV IgM/IgG by ELISA. The detection of HBV DNA and HDV RNA was performed by PCR amplification. The viral genotype was determined by sequencing and phylogenetic analysis. RESULTS: A total of 23/861 individuals were positive for HBsAg detection by rapid test. Serological and/or molecular markers of HDV infection were demonstrated in 43.5 % (10/23) of samples from Amerindians. The phylogenetic analysis demonstrated the exclusive circulation of HBV subgenotype F1b of and HDV 3 in this population. CONCLUSIONS: A high frequency of HBV/HDV infection was found in Amerindian population from Amazonas State, Colombia (43.5 %, 10/23). Nine cases were identified in a population of 861 asymptomatic Amerindian individuals; one symptomatic case (with diagnosis of end-stage hepatic disease) was also identified in the study. The circulation of HDV 3 and HBV subgenotype F1b suggests a constant flow of these viral genotypes as a result of the interaction of the Amerindian populations from Amazon basin. Further studies are necessary to confirm whether HBV subgenotype F1b is the prevalent in the population from South East region in Colombia.
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spelling pubmed-46194132015-10-26 Hepatitis D virus and hepatitis B virus infection in Amerindian communities of the Amazonas state, Colombia di Filippo Villa, Diana Cortes-Mancera, Fabian Payares, Edra Montes, Neyla de la Hoz, Fernando Arbelaez, Maria Patricia Correa, Gonzalo Navas, Maria-Cristina Virol J Research BACKGROUND: In Colombia, cases of Hepatitis D virus (HDV) infection have been officially described since 1985 mainly in Amerindian population from Sierra Nevada de Santa Marta (North Caribbean Coast), Uraba (North West), and Amazon (South East). The last official report of a clinical case of HDV infection in Colombia was registered in 2005. OBJECTIVES: The aims of this study were to identify cases of HDV and/or Hepatitis B virus (HBV) infection in asymptomatic Amerindians from Amazonas state, South East Colombia, and to describe the circulating viral genotypes in this population. STUDY DESIGN: The study population was recruited in 19 Amerindian communities in the Amazonas state. Individuals over 18 years old were screened by rapid test for Hepatitis B surface Antigen (HBsAg). Blood samples obtained from individuals positives for HBsAg in the rapid-test assay were analyzed for HBsAg, anti-HBc, anti-HDV IgM/IgG by ELISA. The detection of HBV DNA and HDV RNA was performed by PCR amplification. The viral genotype was determined by sequencing and phylogenetic analysis. RESULTS: A total of 23/861 individuals were positive for HBsAg detection by rapid test. Serological and/or molecular markers of HDV infection were demonstrated in 43.5 % (10/23) of samples from Amerindians. The phylogenetic analysis demonstrated the exclusive circulation of HBV subgenotype F1b of and HDV 3 in this population. CONCLUSIONS: A high frequency of HBV/HDV infection was found in Amerindian population from Amazonas State, Colombia (43.5 %, 10/23). Nine cases were identified in a population of 861 asymptomatic Amerindian individuals; one symptomatic case (with diagnosis of end-stage hepatic disease) was also identified in the study. The circulation of HDV 3 and HBV subgenotype F1b suggests a constant flow of these viral genotypes as a result of the interaction of the Amerindian populations from Amazon basin. Further studies are necessary to confirm whether HBV subgenotype F1b is the prevalent in the population from South East region in Colombia. BioMed Central 2015-10-24 /pmc/articles/PMC4619413/ /pubmed/26497287 http://dx.doi.org/10.1186/s12985-015-0402-5 Text en © di Filippo Villa et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
di Filippo Villa, Diana
Cortes-Mancera, Fabian
Payares, Edra
Montes, Neyla
de la Hoz, Fernando
Arbelaez, Maria Patricia
Correa, Gonzalo
Navas, Maria-Cristina
Hepatitis D virus and hepatitis B virus infection in Amerindian communities of the Amazonas state, Colombia
title Hepatitis D virus and hepatitis B virus infection in Amerindian communities of the Amazonas state, Colombia
title_full Hepatitis D virus and hepatitis B virus infection in Amerindian communities of the Amazonas state, Colombia
title_fullStr Hepatitis D virus and hepatitis B virus infection in Amerindian communities of the Amazonas state, Colombia
title_full_unstemmed Hepatitis D virus and hepatitis B virus infection in Amerindian communities of the Amazonas state, Colombia
title_short Hepatitis D virus and hepatitis B virus infection in Amerindian communities of the Amazonas state, Colombia
title_sort hepatitis d virus and hepatitis b virus infection in amerindian communities of the amazonas state, colombia
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619413/
https://www.ncbi.nlm.nih.gov/pubmed/26497287
http://dx.doi.org/10.1186/s12985-015-0402-5
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