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Camel milk peptide improves wound healing in diabetic rats by orchestrating the redox status and immune response
BACKGROUND: Diabetes mellitus alters oxidative stability and immune response. Here, we investigated the impact of a peptide extracted from camel milk (CMP) on the oxidative status, transcription factor kappa-B (NF-kB) and inflammatory cytokine in diabetic wounds. METHODS: Rats were assigned into thr...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619484/ https://www.ncbi.nlm.nih.gov/pubmed/26498022 http://dx.doi.org/10.1186/s12944-015-0136-9 |
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author | Ebaid, Hossam Abdel-salam, Bahaa Hassan, Iftekhar Al-Tamimi, Jameel Metwalli, Ali Alhazza, Ibrahim |
author_facet | Ebaid, Hossam Abdel-salam, Bahaa Hassan, Iftekhar Al-Tamimi, Jameel Metwalli, Ali Alhazza, Ibrahim |
author_sort | Ebaid, Hossam |
collection | PubMed |
description | BACKGROUND: Diabetes mellitus alters oxidative stability and immune response. Here, we investigated the impact of a peptide extracted from camel milk (CMP) on the oxidative status, transcription factor kappa-B (NF-kB) and inflammatory cytokine in diabetic wounds. METHODS: Rats were assigned into three groups: control, diabetic induced (DM) and diabetic induced with multiple doses of CMP for a week (DM-CMP). RESULTS: DM showed a sharp decline in the activity of major antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) compared to the control. The DM-CMP group, however, showed a noticeable replenishment in the activity of these enzymes compared to the DM group. The CMP-treated group also showed a normal level of lipid peroxidation marker (MDA) compared to the DM rats. Furthermore, ELISA analysis of serum TNF-α protein showed an elevated level in diabetic rats in comparison to control serum. However, RT-PCR analysis of locally wounded skin tissues revealed that diabetes down-regulates the RNA expression of both TNF-α and MIF genes in comparison to the control samples but that CMP was found to restore RNA expression significantly. Although it was elevated in CMP-treated rats after one day of wound incision, the NF-kB protein level was significantly decreased seven days after the incision in comparison to the animals in the diabetic group. CONCLUSION: CMP, therefore, can be seen an effective antioxidant and immune stimulant that induces oxidative stability and speeds up wound healing in diabetic model animals, making it a potential adjuvant in improving wound healing in those with diabetic conditions. |
format | Online Article Text |
id | pubmed-4619484 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46194842015-10-26 Camel milk peptide improves wound healing in diabetic rats by orchestrating the redox status and immune response Ebaid, Hossam Abdel-salam, Bahaa Hassan, Iftekhar Al-Tamimi, Jameel Metwalli, Ali Alhazza, Ibrahim Lipids Health Dis Research BACKGROUND: Diabetes mellitus alters oxidative stability and immune response. Here, we investigated the impact of a peptide extracted from camel milk (CMP) on the oxidative status, transcription factor kappa-B (NF-kB) and inflammatory cytokine in diabetic wounds. METHODS: Rats were assigned into three groups: control, diabetic induced (DM) and diabetic induced with multiple doses of CMP for a week (DM-CMP). RESULTS: DM showed a sharp decline in the activity of major antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) compared to the control. The DM-CMP group, however, showed a noticeable replenishment in the activity of these enzymes compared to the DM group. The CMP-treated group also showed a normal level of lipid peroxidation marker (MDA) compared to the DM rats. Furthermore, ELISA analysis of serum TNF-α protein showed an elevated level in diabetic rats in comparison to control serum. However, RT-PCR analysis of locally wounded skin tissues revealed that diabetes down-regulates the RNA expression of both TNF-α and MIF genes in comparison to the control samples but that CMP was found to restore RNA expression significantly. Although it was elevated in CMP-treated rats after one day of wound incision, the NF-kB protein level was significantly decreased seven days after the incision in comparison to the animals in the diabetic group. CONCLUSION: CMP, therefore, can be seen an effective antioxidant and immune stimulant that induces oxidative stability and speeds up wound healing in diabetic model animals, making it a potential adjuvant in improving wound healing in those with diabetic conditions. BioMed Central 2015-10-24 /pmc/articles/PMC4619484/ /pubmed/26498022 http://dx.doi.org/10.1186/s12944-015-0136-9 Text en © Ebaid et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Ebaid, Hossam Abdel-salam, Bahaa Hassan, Iftekhar Al-Tamimi, Jameel Metwalli, Ali Alhazza, Ibrahim Camel milk peptide improves wound healing in diabetic rats by orchestrating the redox status and immune response |
title | Camel milk peptide improves wound healing in diabetic rats by orchestrating the redox status and immune response |
title_full | Camel milk peptide improves wound healing in diabetic rats by orchestrating the redox status and immune response |
title_fullStr | Camel milk peptide improves wound healing in diabetic rats by orchestrating the redox status and immune response |
title_full_unstemmed | Camel milk peptide improves wound healing in diabetic rats by orchestrating the redox status and immune response |
title_short | Camel milk peptide improves wound healing in diabetic rats by orchestrating the redox status and immune response |
title_sort | camel milk peptide improves wound healing in diabetic rats by orchestrating the redox status and immune response |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619484/ https://www.ncbi.nlm.nih.gov/pubmed/26498022 http://dx.doi.org/10.1186/s12944-015-0136-9 |
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