Cargando…
Analysis of zebrafish (Danio rerio) behavior in response to bacterial infection using a self-organizing map
BACKGROUND: Animal behavioral responses have been recently established as a suitable tool for detecting contaminants in the environment for risk assessment in situ. In this study, we observed movement behavior of zebrafish (Danio rerio) before and after infection with Edwardsiella tarda CK41 for 3 d...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619490/ https://www.ncbi.nlm.nih.gov/pubmed/26497220 http://dx.doi.org/10.1186/s12917-015-0579-2 |
_version_ | 1782397114722025472 |
---|---|
author | Lee, Sang-Bin Choe, Yunjeong Chon, Tae-Soo Kang, Ho Young |
author_facet | Lee, Sang-Bin Choe, Yunjeong Chon, Tae-Soo Kang, Ho Young |
author_sort | Lee, Sang-Bin |
collection | PubMed |
description | BACKGROUND: Animal behavioral responses have been recently established as a suitable tool for detecting contaminants in the environment for risk assessment in situ. In this study, we observed movement behavior of zebrafish (Danio rerio) before and after infection with Edwardsiella tarda CK41 for 3 days until death. METHODS: Infection status of zebrafish was confirmed through PCR and colonization assay as time progressed and lesion development in the tails of zebrafish was also examined. Movement behaviors in response to bacterial infection were patterned by self-organizing map (SOM) based on movement parameters, including speed (mm/s), acceleration (mm/s(2)), stop duration (t), stop number (n), locomotory rate (mm/s), turning rate (rad/s), and meander (rad/mm). RESULTS: According to SOM result, clusters were identified firstly according to time and secondly according to infection. Two movement patterns were observed in the early period of infection: one group with minimum turning rate and meander (i.e., stiff movement) and the other group with maximum strop number. Late infection was characterized by long stop duration. CONCLUSION: SOM was suitable for extracting complex behavioral data and thus can serve as a referencing system for diagnosing disease development in order to reveal the mechanism of the infection process. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12917-015-0579-2) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4619490 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46194902015-10-26 Analysis of zebrafish (Danio rerio) behavior in response to bacterial infection using a self-organizing map Lee, Sang-Bin Choe, Yunjeong Chon, Tae-Soo Kang, Ho Young BMC Vet Res Research Article BACKGROUND: Animal behavioral responses have been recently established as a suitable tool for detecting contaminants in the environment for risk assessment in situ. In this study, we observed movement behavior of zebrafish (Danio rerio) before and after infection with Edwardsiella tarda CK41 for 3 days until death. METHODS: Infection status of zebrafish was confirmed through PCR and colonization assay as time progressed and lesion development in the tails of zebrafish was also examined. Movement behaviors in response to bacterial infection were patterned by self-organizing map (SOM) based on movement parameters, including speed (mm/s), acceleration (mm/s(2)), stop duration (t), stop number (n), locomotory rate (mm/s), turning rate (rad/s), and meander (rad/mm). RESULTS: According to SOM result, clusters were identified firstly according to time and secondly according to infection. Two movement patterns were observed in the early period of infection: one group with minimum turning rate and meander (i.e., stiff movement) and the other group with maximum strop number. Late infection was characterized by long stop duration. CONCLUSION: SOM was suitable for extracting complex behavioral data and thus can serve as a referencing system for diagnosing disease development in order to reveal the mechanism of the infection process. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12917-015-0579-2) contains supplementary material, which is available to authorized users. BioMed Central 2015-10-23 /pmc/articles/PMC4619490/ /pubmed/26497220 http://dx.doi.org/10.1186/s12917-015-0579-2 Text en © Lee et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Lee, Sang-Bin Choe, Yunjeong Chon, Tae-Soo Kang, Ho Young Analysis of zebrafish (Danio rerio) behavior in response to bacterial infection using a self-organizing map |
title | Analysis of zebrafish (Danio rerio) behavior in response to bacterial infection using a self-organizing map |
title_full | Analysis of zebrafish (Danio rerio) behavior in response to bacterial infection using a self-organizing map |
title_fullStr | Analysis of zebrafish (Danio rerio) behavior in response to bacterial infection using a self-organizing map |
title_full_unstemmed | Analysis of zebrafish (Danio rerio) behavior in response to bacterial infection using a self-organizing map |
title_short | Analysis of zebrafish (Danio rerio) behavior in response to bacterial infection using a self-organizing map |
title_sort | analysis of zebrafish (danio rerio) behavior in response to bacterial infection using a self-organizing map |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619490/ https://www.ncbi.nlm.nih.gov/pubmed/26497220 http://dx.doi.org/10.1186/s12917-015-0579-2 |
work_keys_str_mv | AT leesangbin analysisofzebrafishdanioreriobehaviorinresponsetobacterialinfectionusingaselforganizingmap AT choeyunjeong analysisofzebrafishdanioreriobehaviorinresponsetobacterialinfectionusingaselforganizingmap AT chontaesoo analysisofzebrafishdanioreriobehaviorinresponsetobacterialinfectionusingaselforganizingmap AT kanghoyoung analysisofzebrafishdanioreriobehaviorinresponsetobacterialinfectionusingaselforganizingmap |