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Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells

Quercetin is a dietary flavonoid which exerts anti-oxidant, anti-inflammatory and anti-cancer properties. In this study, we investigated the anti-proliferative effect of quercetin in two breast cancer cell lines (MCF-7 and MDA-MB-231), which differed in hormone receptor. IC(50) value (37μM) of querc...

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Autores principales: Ranganathan, Santhalakshmi, Halagowder, Devaraj, Sivasithambaram, Niranjali Devaraj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619597/
https://www.ncbi.nlm.nih.gov/pubmed/26491966
http://dx.doi.org/10.1371/journal.pone.0141370
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author Ranganathan, Santhalakshmi
Halagowder, Devaraj
Sivasithambaram, Niranjali Devaraj
author_facet Ranganathan, Santhalakshmi
Halagowder, Devaraj
Sivasithambaram, Niranjali Devaraj
author_sort Ranganathan, Santhalakshmi
collection PubMed
description Quercetin is a dietary flavonoid which exerts anti-oxidant, anti-inflammatory and anti-cancer properties. In this study, we investigated the anti-proliferative effect of quercetin in two breast cancer cell lines (MCF-7 and MDA-MB-231), which differed in hormone receptor. IC(50) value (37μM) of quercetin showed significant cytotoxicity in MCF-7 cells, which was not observed in MDA-MB-231 cells even at 100μM of quercetin treatment. To study the response of cancer cells to quercetin, with respect to different hormone receptors, both the cell lines were treated with a fixed concentration (40μM) of quercetin. MCF-7 cells on quercetin treatment showed more apoptotic cells with G1 phase arrest. In addition, quercetin effectively suppressed the expression of CyclinD1, p21, Twist and phospho p38MAPK, which was not observed in MDA-MB-231 cells. To analyse the molecular mechanism of quercetin in exerting an apoptotic effect in MCF-7 cells, Twist was over-expressed and the molecular changes were observed after quercetin administration. Quercetin effectively regulated the expression of Twist, in turn p16 and p21 which induced apoptosis in MCF-7 cells. In conclusion, quercetin induces apoptosis in breast cancer cells through suppression of Twist via p38MAPK pathway.
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spelling pubmed-46195972015-10-29 Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells Ranganathan, Santhalakshmi Halagowder, Devaraj Sivasithambaram, Niranjali Devaraj PLoS One Research Article Quercetin is a dietary flavonoid which exerts anti-oxidant, anti-inflammatory and anti-cancer properties. In this study, we investigated the anti-proliferative effect of quercetin in two breast cancer cell lines (MCF-7 and MDA-MB-231), which differed in hormone receptor. IC(50) value (37μM) of quercetin showed significant cytotoxicity in MCF-7 cells, which was not observed in MDA-MB-231 cells even at 100μM of quercetin treatment. To study the response of cancer cells to quercetin, with respect to different hormone receptors, both the cell lines were treated with a fixed concentration (40μM) of quercetin. MCF-7 cells on quercetin treatment showed more apoptotic cells with G1 phase arrest. In addition, quercetin effectively suppressed the expression of CyclinD1, p21, Twist and phospho p38MAPK, which was not observed in MDA-MB-231 cells. To analyse the molecular mechanism of quercetin in exerting an apoptotic effect in MCF-7 cells, Twist was over-expressed and the molecular changes were observed after quercetin administration. Quercetin effectively regulated the expression of Twist, in turn p16 and p21 which induced apoptosis in MCF-7 cells. In conclusion, quercetin induces apoptosis in breast cancer cells through suppression of Twist via p38MAPK pathway. Public Library of Science 2015-10-22 /pmc/articles/PMC4619597/ /pubmed/26491966 http://dx.doi.org/10.1371/journal.pone.0141370 Text en © 2015 Ranganathan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ranganathan, Santhalakshmi
Halagowder, Devaraj
Sivasithambaram, Niranjali Devaraj
Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells
title Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells
title_full Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells
title_fullStr Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells
title_full_unstemmed Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells
title_short Quercetin Suppresses Twist to Induce Apoptosis in MCF-7 Breast Cancer Cells
title_sort quercetin suppresses twist to induce apoptosis in mcf-7 breast cancer cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619597/
https://www.ncbi.nlm.nih.gov/pubmed/26491966
http://dx.doi.org/10.1371/journal.pone.0141370
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