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Efficient Mating-Type Switching in Candida glabrata Induces Cell Death

Candida glabrata is an apparently asexual haploid yeast that is phylogenetically closer to Saccharomyces cerevisiae than to Candida albicans. Its genome contains three MAT-like cassettes, MAT, which encodes either MATa or MATalpha information in different strains, and the additional loci, HML and HM...

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Autores principales: Boisnard, Stéphanie, Zhou Li, Youfang, Arnaise, Sylvie, Sequeira, Gregory, Raffoux, Xavier, Enache-Angoulvant, Adela, Bolotin-Fukuhara, Monique, Fairhead, Cécile
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619647/
https://www.ncbi.nlm.nih.gov/pubmed/26491872
http://dx.doi.org/10.1371/journal.pone.0140990
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author Boisnard, Stéphanie
Zhou Li, Youfang
Arnaise, Sylvie
Sequeira, Gregory
Raffoux, Xavier
Enache-Angoulvant, Adela
Bolotin-Fukuhara, Monique
Fairhead, Cécile
author_facet Boisnard, Stéphanie
Zhou Li, Youfang
Arnaise, Sylvie
Sequeira, Gregory
Raffoux, Xavier
Enache-Angoulvant, Adela
Bolotin-Fukuhara, Monique
Fairhead, Cécile
author_sort Boisnard, Stéphanie
collection PubMed
description Candida glabrata is an apparently asexual haploid yeast that is phylogenetically closer to Saccharomyces cerevisiae than to Candida albicans. Its genome contains three MAT-like cassettes, MAT, which encodes either MATa or MATalpha information in different strains, and the additional loci, HML and HMR. The genome also contains an HO gene homolog, but this yeast has never been shown to switch mating-types spontaneously, as S. cerevisiae does. We have recently sequenced the genomes of the five species that, together with C. glabrata, make up the Nakaseomyces clade. All contain MAT-like cassettes and an HO gene homolog. In this work, we express the HO gene of all Nakaseomyces and of S. cerevisiae in C. glabrata. All can induce mating-type switching, but, despite the larger phylogenetic distance, the most efficient endonuclease is the one from S. cerevisiae. Efficient mating-type switching in C. glabrata is accompanied by a high cell mortality, and sometimes results in conversion of the additional cassette HML. Mortality probably results from the cutting of the HO recognition sites that are present, in HML and possibly HMR, contrary to what happens naturally in S. cerevisiae. This has implications in the life-cycle of C. glabrata, as we show that efficient MAT switching is lethal for most cells, induces chromosomal rearrangements in survivors, and that the endogenous HO is probably rarely active indeed.
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spelling pubmed-46196472015-10-29 Efficient Mating-Type Switching in Candida glabrata Induces Cell Death Boisnard, Stéphanie Zhou Li, Youfang Arnaise, Sylvie Sequeira, Gregory Raffoux, Xavier Enache-Angoulvant, Adela Bolotin-Fukuhara, Monique Fairhead, Cécile PLoS One Research Article Candida glabrata is an apparently asexual haploid yeast that is phylogenetically closer to Saccharomyces cerevisiae than to Candida albicans. Its genome contains three MAT-like cassettes, MAT, which encodes either MATa or MATalpha information in different strains, and the additional loci, HML and HMR. The genome also contains an HO gene homolog, but this yeast has never been shown to switch mating-types spontaneously, as S. cerevisiae does. We have recently sequenced the genomes of the five species that, together with C. glabrata, make up the Nakaseomyces clade. All contain MAT-like cassettes and an HO gene homolog. In this work, we express the HO gene of all Nakaseomyces and of S. cerevisiae in C. glabrata. All can induce mating-type switching, but, despite the larger phylogenetic distance, the most efficient endonuclease is the one from S. cerevisiae. Efficient mating-type switching in C. glabrata is accompanied by a high cell mortality, and sometimes results in conversion of the additional cassette HML. Mortality probably results from the cutting of the HO recognition sites that are present, in HML and possibly HMR, contrary to what happens naturally in S. cerevisiae. This has implications in the life-cycle of C. glabrata, as we show that efficient MAT switching is lethal for most cells, induces chromosomal rearrangements in survivors, and that the endogenous HO is probably rarely active indeed. Public Library of Science 2015-10-22 /pmc/articles/PMC4619647/ /pubmed/26491872 http://dx.doi.org/10.1371/journal.pone.0140990 Text en © 2015 Boisnard et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Boisnard, Stéphanie
Zhou Li, Youfang
Arnaise, Sylvie
Sequeira, Gregory
Raffoux, Xavier
Enache-Angoulvant, Adela
Bolotin-Fukuhara, Monique
Fairhead, Cécile
Efficient Mating-Type Switching in Candida glabrata Induces Cell Death
title Efficient Mating-Type Switching in Candida glabrata Induces Cell Death
title_full Efficient Mating-Type Switching in Candida glabrata Induces Cell Death
title_fullStr Efficient Mating-Type Switching in Candida glabrata Induces Cell Death
title_full_unstemmed Efficient Mating-Type Switching in Candida glabrata Induces Cell Death
title_short Efficient Mating-Type Switching in Candida glabrata Induces Cell Death
title_sort efficient mating-type switching in candida glabrata induces cell death
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619647/
https://www.ncbi.nlm.nih.gov/pubmed/26491872
http://dx.doi.org/10.1371/journal.pone.0140990
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