Monitoring of the Parasite Load in the Digestive Tract of Rhodnius prolixus by Combined qPCR Analysis and Imaging Techniques Provides New Insights into the Trypanosome Life Cycle

BACKGROUND: Here we report the monitoring of the digestive tract colonization of Rhodnius prolixus by Trypanosoma cruzi using an accurate determination of the parasite load by qPCR coupled with fluorescence and bioluminescence imaging (BLI). These complementary methods revealed critical steps necess...

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Detalles Bibliográficos
Autores principales: Dias, Felipe de Almeida, Guerra, Barbara, Vieira, Larissa Rezende, Perdomo, Hugo Diego, Gandara, Ana Caroline Paiva, do Amaral, Raquel Juliana Vionette, Vollú, Renata Estebanez, Gomes, Suzete Araujo Oliveira, Lara, Flavio Alves, Sorgine, Marcos Henrique Ferreira, Medei, Emiliano, de Oliveira, Pedro Lagerblad, Salmon, Didier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619730/
https://www.ncbi.nlm.nih.gov/pubmed/26496442
http://dx.doi.org/10.1371/journal.pntd.0004186

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