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The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells

The introduction of genes into glial cells for mechanistic studies of cell function and as a therapeutic for gene delivery is an expanding field. Though viral vector based systems do exhibit good delivery efficiency and long-term production of the transgene, the need for transient gene expression, b...

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Autores principales: Rao, Sudheendra, Morales, Alejo A., Pearse, Damien D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619820/
https://www.ncbi.nlm.nih.gov/pubmed/26539498
http://dx.doi.org/10.1155/2015/458624
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author Rao, Sudheendra
Morales, Alejo A.
Pearse, Damien D.
author_facet Rao, Sudheendra
Morales, Alejo A.
Pearse, Damien D.
author_sort Rao, Sudheendra
collection PubMed
description The introduction of genes into glial cells for mechanistic studies of cell function and as a therapeutic for gene delivery is an expanding field. Though viral vector based systems do exhibit good delivery efficiency and long-term production of the transgene, the need for transient gene expression, broad and rapid gene setup methodologies, and safety concerns regarding in vivo application still incentivize research into the use of nonviral gene delivery methods. In the current study, aviral gene delivery vectors based upon cationic lipid (Lipofectamine 3000) lipoplex or polyethylenimine (Viromer RED) polyplex technologies were examined in cell lines and primary glial cells for their transfection efficiencies, gene expression levels, and toxicity. The transfection efficiencies of polyplex and lipoplex agents were found to be comparable in a limited, yet similar, transfection setting, with or without serum across a number of cell types. However, differential effects on cell-specific transgene expression and reduced viability with cargo loaded polyplex were observed. Overall, our data suggests that polyplex technology could perform comparably to the market dominant lipoplex technology in transfecting various cells lines including glial cells but also stress a need for further refinement of polyplex reagents to minimize their effects on cell viability.
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spelling pubmed-46198202015-11-04 The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells Rao, Sudheendra Morales, Alejo A. Pearse, Damien D. Biomed Res Int Research Article The introduction of genes into glial cells for mechanistic studies of cell function and as a therapeutic for gene delivery is an expanding field. Though viral vector based systems do exhibit good delivery efficiency and long-term production of the transgene, the need for transient gene expression, broad and rapid gene setup methodologies, and safety concerns regarding in vivo application still incentivize research into the use of nonviral gene delivery methods. In the current study, aviral gene delivery vectors based upon cationic lipid (Lipofectamine 3000) lipoplex or polyethylenimine (Viromer RED) polyplex technologies were examined in cell lines and primary glial cells for their transfection efficiencies, gene expression levels, and toxicity. The transfection efficiencies of polyplex and lipoplex agents were found to be comparable in a limited, yet similar, transfection setting, with or without serum across a number of cell types. However, differential effects on cell-specific transgene expression and reduced viability with cargo loaded polyplex were observed. Overall, our data suggests that polyplex technology could perform comparably to the market dominant lipoplex technology in transfecting various cells lines including glial cells but also stress a need for further refinement of polyplex reagents to minimize their effects on cell viability. Hindawi Publishing Corporation 2015 2015-10-11 /pmc/articles/PMC4619820/ /pubmed/26539498 http://dx.doi.org/10.1155/2015/458624 Text en Copyright © 2015 Sudheendra Rao et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rao, Sudheendra
Morales, Alejo A.
Pearse, Damien D.
The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells
title The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells
title_full The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells
title_fullStr The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells
title_full_unstemmed The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells
title_short The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells
title_sort comparative utility of viromer red and lipofectamine for transient gene introduction into glial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619820/
https://www.ncbi.nlm.nih.gov/pubmed/26539498
http://dx.doi.org/10.1155/2015/458624
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