Amylose-Based Cationic Star Polymers for siRNA Delivery

A new siRNA delivery system using a cationic glyco-star polymer is described. Spermine-modified 8-arm amylose star polymer (with a degree of polymerization of approximately 60 per arm) was synthesized by chemoenzymatic methods. The cationic star polymer effectively bound to siRNA and formed spherica...

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Detalles Bibliográficos
Autores principales: Nishimura, Tomoki, Umezaki, Kaori, Mukai, Sada-atsu, Sawada, Shin-ichi, Akiyoshi, Kazunari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619918/
https://www.ncbi.nlm.nih.gov/pubmed/26539548
http://dx.doi.org/10.1155/2015/962941
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author Nishimura, Tomoki
Umezaki, Kaori
Mukai, Sada-atsu
Sawada, Shin-ichi
Akiyoshi, Kazunari
author_facet Nishimura, Tomoki
Umezaki, Kaori
Mukai, Sada-atsu
Sawada, Shin-ichi
Akiyoshi, Kazunari
author_sort Nishimura, Tomoki
collection PubMed
description A new siRNA delivery system using a cationic glyco-star polymer is described. Spermine-modified 8-arm amylose star polymer (with a degree of polymerization of approximately 60 per arm) was synthesized by chemoenzymatic methods. The cationic star polymer effectively bound to siRNA and formed spherical complexes with an average hydrodynamic diameter of 230 nm. The cationic 8-arm star polymer complexes showed superior cellular uptake characteristics and higher gene silencing effects than a cationic 1-arm polymer. These results suggest that amylose-based star polymers are a promising nanoplatform for glycobiomaterials.
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spelling pubmed-46199182015-11-04 Amylose-Based Cationic Star Polymers for siRNA Delivery Nishimura, Tomoki Umezaki, Kaori Mukai, Sada-atsu Sawada, Shin-ichi Akiyoshi, Kazunari Biomed Res Int Research Article A new siRNA delivery system using a cationic glyco-star polymer is described. Spermine-modified 8-arm amylose star polymer (with a degree of polymerization of approximately 60 per arm) was synthesized by chemoenzymatic methods. The cationic star polymer effectively bound to siRNA and formed spherical complexes with an average hydrodynamic diameter of 230 nm. The cationic 8-arm star polymer complexes showed superior cellular uptake characteristics and higher gene silencing effects than a cationic 1-arm polymer. These results suggest that amylose-based star polymers are a promising nanoplatform for glycobiomaterials. Hindawi Publishing Corporation 2015 2015-10-11 /pmc/articles/PMC4619918/ /pubmed/26539548 http://dx.doi.org/10.1155/2015/962941 Text en Copyright © 2015 Tomoki Nishimura et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nishimura, Tomoki
Umezaki, Kaori
Mukai, Sada-atsu
Sawada, Shin-ichi
Akiyoshi, Kazunari
Amylose-Based Cationic Star Polymers for siRNA Delivery
title Amylose-Based Cationic Star Polymers for siRNA Delivery
title_full Amylose-Based Cationic Star Polymers for siRNA Delivery
title_fullStr Amylose-Based Cationic Star Polymers for siRNA Delivery
title_full_unstemmed Amylose-Based Cationic Star Polymers for siRNA Delivery
title_short Amylose-Based Cationic Star Polymers for siRNA Delivery
title_sort amylose-based cationic star polymers for sirna delivery
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619918/
https://www.ncbi.nlm.nih.gov/pubmed/26539548
http://dx.doi.org/10.1155/2015/962941
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AT mukaisadaatsu amylosebasedcationicstarpolymersforsirnadelivery
AT sawadashinichi amylosebasedcationicstarpolymersforsirnadelivery
AT akiyoshikazunari amylosebasedcationicstarpolymersforsirnadelivery

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