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Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides
GC-MS method combined with FTIR techniques by the analysis of polysaccharide was applied to evaluate the similarity between wild (W) and tissue-cultured (TC) Dendrobium huoshanense (DHS), Dendrobium officinale (DO), and Dendrobium moniliforme (DM) as well as 3 wild Dendrobium spp.: Dendrobium henane...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619951/ https://www.ncbi.nlm.nih.gov/pubmed/26539215 http://dx.doi.org/10.1155/2015/713410 |
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author | Chen, Nai-Dong Chen, Nai-Fu Li, Jun Cao, Cai-Yun Wang, Jin-Mei Huang, He-Ping |
author_facet | Chen, Nai-Dong Chen, Nai-Fu Li, Jun Cao, Cai-Yun Wang, Jin-Mei Huang, He-Ping |
author_sort | Chen, Nai-Dong |
collection | PubMed |
description | GC-MS method combined with FTIR techniques by the analysis of polysaccharide was applied to evaluate the similarity between wild (W) and tissue-cultured (TC) Dendrobium huoshanense (DHS), Dendrobium officinale (DO), and Dendrobium moniliforme (DM) as well as 3 wild Dendrobium spp.: Dendrobium henanense (DHN), Dendrobium loddigesii (DL), and Dendrobium crepidatum (DC). Eight monosaccharides involving xylose, arabinose, rhamnose, glucose, mannose, fructose, galactose, and galacturonic acid were identified in the polysaccharide from each Dendrobium sample while the contents of the monosugars varied remarkably across origins and species. Further similarity evaluation based on GC-MS data showed that the r (cor) values of different origins of DHS, DO, and DM were 0.831, 0.865, and 0.884, respectively, while the r (cor) values ranged from 0.475 to 0.837 across species. FTIR files of the polysaccharides revealed that the similarity coefficients between W and TC-DHS, DO, and DM were 88.7%, 86.8%, and 88.5%, respectively, in contrast to the similarity coefficients varying from 57.4% to 82.6% across species. These results suggested that the structures of polysaccharides between different origins of the investigated Dendrobiums might be higher than what we had supposed. |
format | Online Article Text |
id | pubmed-4619951 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-46199512015-11-04 Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides Chen, Nai-Dong Chen, Nai-Fu Li, Jun Cao, Cai-Yun Wang, Jin-Mei Huang, He-Ping Int J Anal Chem Research Article GC-MS method combined with FTIR techniques by the analysis of polysaccharide was applied to evaluate the similarity between wild (W) and tissue-cultured (TC) Dendrobium huoshanense (DHS), Dendrobium officinale (DO), and Dendrobium moniliforme (DM) as well as 3 wild Dendrobium spp.: Dendrobium henanense (DHN), Dendrobium loddigesii (DL), and Dendrobium crepidatum (DC). Eight monosaccharides involving xylose, arabinose, rhamnose, glucose, mannose, fructose, galactose, and galacturonic acid were identified in the polysaccharide from each Dendrobium sample while the contents of the monosugars varied remarkably across origins and species. Further similarity evaluation based on GC-MS data showed that the r (cor) values of different origins of DHS, DO, and DM were 0.831, 0.865, and 0.884, respectively, while the r (cor) values ranged from 0.475 to 0.837 across species. FTIR files of the polysaccharides revealed that the similarity coefficients between W and TC-DHS, DO, and DM were 88.7%, 86.8%, and 88.5%, respectively, in contrast to the similarity coefficients varying from 57.4% to 82.6% across species. These results suggested that the structures of polysaccharides between different origins of the investigated Dendrobiums might be higher than what we had supposed. Hindawi Publishing Corporation 2015 2015-10-11 /pmc/articles/PMC4619951/ /pubmed/26539215 http://dx.doi.org/10.1155/2015/713410 Text en Copyright © 2015 Nai-Dong Chen et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chen, Nai-Dong Chen, Nai-Fu Li, Jun Cao, Cai-Yun Wang, Jin-Mei Huang, He-Ping Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides |
title | Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides |
title_full | Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides |
title_fullStr | Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides |
title_full_unstemmed | Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides |
title_short | Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides |
title_sort | similarity evaluation of different origins and species of dendrobiums by gc-ms and ftir analysis of polysaccharides |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619951/ https://www.ncbi.nlm.nih.gov/pubmed/26539215 http://dx.doi.org/10.1155/2015/713410 |
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