Cargando…
Clonorchis sinensis excretory/secretory products promote the secretion of TNF-alpha in the mouse intrahepatic biliary epithelial cells via Toll-like receptor 4
BACKGROUND: Toll-like receptor 4 (TLR4), as one of the most important pathogen pattern recognitions (PPRs) plays a central role in elicitation of innate immunity and mediation of adaptive responses against foreign antigens. However, little is known of the roles of TLR4 in the immune responses of bil...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4620022/ https://www.ncbi.nlm.nih.gov/pubmed/26497121 http://dx.doi.org/10.1186/s13071-015-1171-0 |
_version_ | 1782397236214235136 |
---|---|
author | Yan, Chao Wang, Yan-Hong Yu, Qian Cheng, Xiao-Dan Zhang, Bei-Bei Li, Bo Zhang, Bo Tang, Ren-Xian Zheng, Kui-Yang |
author_facet | Yan, Chao Wang, Yan-Hong Yu, Qian Cheng, Xiao-Dan Zhang, Bei-Bei Li, Bo Zhang, Bo Tang, Ren-Xian Zheng, Kui-Yang |
author_sort | Yan, Chao |
collection | PubMed |
description | BACKGROUND: Toll-like receptor 4 (TLR4), as one of the most important pathogen pattern recognitions (PPRs) plays a central role in elicitation of innate immunity and mediation of adaptive responses against foreign antigens. However, little is known of the roles of TLR4 in the immune responses of biliary epithelial cells (BECs) induced by Clonorchis sinensis, a parasite of significance in human health. METHODS: In the present study, the primary mouse intrahepatic biliary epithelial cells (MIBECs) were pre-treated with TLR4 inhibitor peptide or control peptide and then stimulated by excretory/secretory products (ESP) of C. sinensis, respectively. The expressions of TLR4 and relative cytokines were determined using western blot and a bead-based analytic detection system, respectively. RESULTS: The results showed that ESP of C. sinensis significantly increased the expression of TLR4 which promoted the expression of MyD88 and NF-κB in BECs; the levels of TNF-α but not IL-6 from MIBECs stimulated by ESP alone were also considerably increased, compared with the group of the medium stimulated. However, the concentration of TNF-α was significantly decreased when MIBECs were pre-treated with TLR4 inhibitor. In addition, ESP could depress the level of IL-6 in MIBECs which was elevated by LPS. CONCLUSIONS: Our data for the first time demonstrate that ESP of C. sinensis can potently induce secretion of pro-inflammatory cytokines via TLR4 in MIBECs, which suggests that TLR4 plays an important role in host defenses against C. sinensis and the pathogenesis of clonorchiasis. |
format | Online Article Text |
id | pubmed-4620022 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46200222015-10-26 Clonorchis sinensis excretory/secretory products promote the secretion of TNF-alpha in the mouse intrahepatic biliary epithelial cells via Toll-like receptor 4 Yan, Chao Wang, Yan-Hong Yu, Qian Cheng, Xiao-Dan Zhang, Bei-Bei Li, Bo Zhang, Bo Tang, Ren-Xian Zheng, Kui-Yang Parasit Vectors Research BACKGROUND: Toll-like receptor 4 (TLR4), as one of the most important pathogen pattern recognitions (PPRs) plays a central role in elicitation of innate immunity and mediation of adaptive responses against foreign antigens. However, little is known of the roles of TLR4 in the immune responses of biliary epithelial cells (BECs) induced by Clonorchis sinensis, a parasite of significance in human health. METHODS: In the present study, the primary mouse intrahepatic biliary epithelial cells (MIBECs) were pre-treated with TLR4 inhibitor peptide or control peptide and then stimulated by excretory/secretory products (ESP) of C. sinensis, respectively. The expressions of TLR4 and relative cytokines were determined using western blot and a bead-based analytic detection system, respectively. RESULTS: The results showed that ESP of C. sinensis significantly increased the expression of TLR4 which promoted the expression of MyD88 and NF-κB in BECs; the levels of TNF-α but not IL-6 from MIBECs stimulated by ESP alone were also considerably increased, compared with the group of the medium stimulated. However, the concentration of TNF-α was significantly decreased when MIBECs were pre-treated with TLR4 inhibitor. In addition, ESP could depress the level of IL-6 in MIBECs which was elevated by LPS. CONCLUSIONS: Our data for the first time demonstrate that ESP of C. sinensis can potently induce secretion of pro-inflammatory cytokines via TLR4 in MIBECs, which suggests that TLR4 plays an important role in host defenses against C. sinensis and the pathogenesis of clonorchiasis. BioMed Central 2015-10-24 /pmc/articles/PMC4620022/ /pubmed/26497121 http://dx.doi.org/10.1186/s13071-015-1171-0 Text en © Yan et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Yan, Chao Wang, Yan-Hong Yu, Qian Cheng, Xiao-Dan Zhang, Bei-Bei Li, Bo Zhang, Bo Tang, Ren-Xian Zheng, Kui-Yang Clonorchis sinensis excretory/secretory products promote the secretion of TNF-alpha in the mouse intrahepatic biliary epithelial cells via Toll-like receptor 4 |
title | Clonorchis sinensis excretory/secretory products promote the secretion of TNF-alpha in the mouse intrahepatic biliary epithelial cells via Toll-like receptor 4 |
title_full | Clonorchis sinensis excretory/secretory products promote the secretion of TNF-alpha in the mouse intrahepatic biliary epithelial cells via Toll-like receptor 4 |
title_fullStr | Clonorchis sinensis excretory/secretory products promote the secretion of TNF-alpha in the mouse intrahepatic biliary epithelial cells via Toll-like receptor 4 |
title_full_unstemmed | Clonorchis sinensis excretory/secretory products promote the secretion of TNF-alpha in the mouse intrahepatic biliary epithelial cells via Toll-like receptor 4 |
title_short | Clonorchis sinensis excretory/secretory products promote the secretion of TNF-alpha in the mouse intrahepatic biliary epithelial cells via Toll-like receptor 4 |
title_sort | clonorchis sinensis excretory/secretory products promote the secretion of tnf-alpha in the mouse intrahepatic biliary epithelial cells via toll-like receptor 4 |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4620022/ https://www.ncbi.nlm.nih.gov/pubmed/26497121 http://dx.doi.org/10.1186/s13071-015-1171-0 |
work_keys_str_mv | AT yanchao clonorchissinensisexcretorysecretoryproductspromotethesecretionoftnfalphainthemouseintrahepaticbiliaryepithelialcellsviatolllikereceptor4 AT wangyanhong clonorchissinensisexcretorysecretoryproductspromotethesecretionoftnfalphainthemouseintrahepaticbiliaryepithelialcellsviatolllikereceptor4 AT yuqian clonorchissinensisexcretorysecretoryproductspromotethesecretionoftnfalphainthemouseintrahepaticbiliaryepithelialcellsviatolllikereceptor4 AT chengxiaodan clonorchissinensisexcretorysecretoryproductspromotethesecretionoftnfalphainthemouseintrahepaticbiliaryepithelialcellsviatolllikereceptor4 AT zhangbeibei clonorchissinensisexcretorysecretoryproductspromotethesecretionoftnfalphainthemouseintrahepaticbiliaryepithelialcellsviatolllikereceptor4 AT libo clonorchissinensisexcretorysecretoryproductspromotethesecretionoftnfalphainthemouseintrahepaticbiliaryepithelialcellsviatolllikereceptor4 AT zhangbo clonorchissinensisexcretorysecretoryproductspromotethesecretionoftnfalphainthemouseintrahepaticbiliaryepithelialcellsviatolllikereceptor4 AT tangrenxian clonorchissinensisexcretorysecretoryproductspromotethesecretionoftnfalphainthemouseintrahepaticbiliaryepithelialcellsviatolllikereceptor4 AT zhengkuiyang clonorchissinensisexcretorysecretoryproductspromotethesecretionoftnfalphainthemouseintrahepaticbiliaryepithelialcellsviatolllikereceptor4 |