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Disruption of diapause induction by TALEN-based gene mutagenesis in relation to a unique neuropeptide signaling pathway in Bombyx

The insect neuropeptide family FXPRLa, which carries the Phe-Xaa-Pro-Arg-Leu-NH(2) sequence at the C-terminus, is involved in many physiological processes. Although ligand–receptor interactions in FXPRLa signaling have been examined using in vitro assays, the correlation between these interactions a...

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Autores principales: Shiomi, Kunihiro, Takasu, Yoko, Kunii, Masayo, Tsuchiya, Ryoma, Mukaida, Moeka, Kobayashi, Masakazu, Sezutsu, Hideki, Ichida Takahama, Masatoshi, Mizoguchi, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4620438/
https://www.ncbi.nlm.nih.gov/pubmed/26497859
http://dx.doi.org/10.1038/srep15566
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author Shiomi, Kunihiro
Takasu, Yoko
Kunii, Masayo
Tsuchiya, Ryoma
Mukaida, Moeka
Kobayashi, Masakazu
Sezutsu, Hideki
Ichida Takahama, Masatoshi
Mizoguchi, Akira
author_facet Shiomi, Kunihiro
Takasu, Yoko
Kunii, Masayo
Tsuchiya, Ryoma
Mukaida, Moeka
Kobayashi, Masakazu
Sezutsu, Hideki
Ichida Takahama, Masatoshi
Mizoguchi, Akira
author_sort Shiomi, Kunihiro
collection PubMed
description The insect neuropeptide family FXPRLa, which carries the Phe-Xaa-Pro-Arg-Leu-NH(2) sequence at the C-terminus, is involved in many physiological processes. Although ligand–receptor interactions in FXPRLa signaling have been examined using in vitro assays, the correlation between these interactions and in vivo physiological function is unclear. Diapause in the silkworm, Bombyx mori, is thought to be elicited by diapause hormone (DH, an FXPRLa) signaling, which consists of interactions between DH and DH receptor (DHR). Here, we performed transcription activator-like effector nuclease (TALEN)-based mutagenesis of the Bombyx DH-PBAN and DHR genes and isolated the null mutants of these genes in a bivoltine strain. All mutant silkworms were fully viable and showed no abnormalities in the developmental timing of ecdysis or metamorphosis. However, female adults oviposited non-diapause eggs despite diapause-inducing temperature and photoperiod conditions. Therefore, we conclude that DH signaling is essential for diapause induction and consists of highly sensitive and specific interactions between DH and DHR selected during ligand–receptor coevolution in Bombyx mori.
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spelling pubmed-46204382015-10-29 Disruption of diapause induction by TALEN-based gene mutagenesis in relation to a unique neuropeptide signaling pathway in Bombyx Shiomi, Kunihiro Takasu, Yoko Kunii, Masayo Tsuchiya, Ryoma Mukaida, Moeka Kobayashi, Masakazu Sezutsu, Hideki Ichida Takahama, Masatoshi Mizoguchi, Akira Sci Rep Article The insect neuropeptide family FXPRLa, which carries the Phe-Xaa-Pro-Arg-Leu-NH(2) sequence at the C-terminus, is involved in many physiological processes. Although ligand–receptor interactions in FXPRLa signaling have been examined using in vitro assays, the correlation between these interactions and in vivo physiological function is unclear. Diapause in the silkworm, Bombyx mori, is thought to be elicited by diapause hormone (DH, an FXPRLa) signaling, which consists of interactions between DH and DH receptor (DHR). Here, we performed transcription activator-like effector nuclease (TALEN)-based mutagenesis of the Bombyx DH-PBAN and DHR genes and isolated the null mutants of these genes in a bivoltine strain. All mutant silkworms were fully viable and showed no abnormalities in the developmental timing of ecdysis or metamorphosis. However, female adults oviposited non-diapause eggs despite diapause-inducing temperature and photoperiod conditions. Therefore, we conclude that DH signaling is essential for diapause induction and consists of highly sensitive and specific interactions between DH and DHR selected during ligand–receptor coevolution in Bombyx mori. Nature Publishing Group 2015-10-26 /pmc/articles/PMC4620438/ /pubmed/26497859 http://dx.doi.org/10.1038/srep15566 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Shiomi, Kunihiro
Takasu, Yoko
Kunii, Masayo
Tsuchiya, Ryoma
Mukaida, Moeka
Kobayashi, Masakazu
Sezutsu, Hideki
Ichida Takahama, Masatoshi
Mizoguchi, Akira
Disruption of diapause induction by TALEN-based gene mutagenesis in relation to a unique neuropeptide signaling pathway in Bombyx
title Disruption of diapause induction by TALEN-based gene mutagenesis in relation to a unique neuropeptide signaling pathway in Bombyx
title_full Disruption of diapause induction by TALEN-based gene mutagenesis in relation to a unique neuropeptide signaling pathway in Bombyx
title_fullStr Disruption of diapause induction by TALEN-based gene mutagenesis in relation to a unique neuropeptide signaling pathway in Bombyx
title_full_unstemmed Disruption of diapause induction by TALEN-based gene mutagenesis in relation to a unique neuropeptide signaling pathway in Bombyx
title_short Disruption of diapause induction by TALEN-based gene mutagenesis in relation to a unique neuropeptide signaling pathway in Bombyx
title_sort disruption of diapause induction by talen-based gene mutagenesis in relation to a unique neuropeptide signaling pathway in bombyx
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4620438/
https://www.ncbi.nlm.nih.gov/pubmed/26497859
http://dx.doi.org/10.1038/srep15566
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