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Spatially Distinct Neutrophil Responses within the Inflammatory Lesions of Pneumonic Plague

During pneumonic plague, the bacterium Yersinia pestis elicits the development of inflammatory lung lesions that continue to expand throughout infection. This lesion development and persistence are poorly understood. Here, we examine spatially distinct regions of lung lesions using laser capture mic...

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Autores principales: Stasulli, Nikolas M., Eichelberger, Kara R., Price, Paul A., Pechous, Roger D., Montgomery, Stephanie A., Parker, Joel S., Goldman, William E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Microbiology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4620470/
https://www.ncbi.nlm.nih.gov/pubmed/26463167
http://dx.doi.org/10.1128/mBio.01530-15
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author Stasulli, Nikolas M.
Eichelberger, Kara R.
Price, Paul A.
Pechous, Roger D.
Montgomery, Stephanie A.
Parker, Joel S.
Goldman, William E.
author_facet Stasulli, Nikolas M.
Eichelberger, Kara R.
Price, Paul A.
Pechous, Roger D.
Montgomery, Stephanie A.
Parker, Joel S.
Goldman, William E.
author_sort Stasulli, Nikolas M.
collection PubMed
description During pneumonic plague, the bacterium Yersinia pestis elicits the development of inflammatory lung lesions that continue to expand throughout infection. This lesion development and persistence are poorly understood. Here, we examine spatially distinct regions of lung lesions using laser capture microdissection and transcriptome sequencing (RNA-seq) analysis to identify transcriptional differences between lesion microenvironments. We show that cellular pathways involved in leukocyte migration and apoptosis are downregulated in the center of lung lesions compared to the periphery. Probing for the bacterial factor(s) important for the alteration in neutrophil survival, we show both in vitro and in vivo that Y. pestis increases neutrophil survival in a manner that is dependent on the type III secretion system effector YopM. This research explores the complexity of spatially distinct host-microbe interactions and emphasizes the importance of cell relevance in assays in order to fully understand Y. pestis virulence.
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spelling pubmed-46204702015-10-26 Spatially Distinct Neutrophil Responses within the Inflammatory Lesions of Pneumonic Plague Stasulli, Nikolas M. Eichelberger, Kara R. Price, Paul A. Pechous, Roger D. Montgomery, Stephanie A. Parker, Joel S. Goldman, William E. mBio Research Article During pneumonic plague, the bacterium Yersinia pestis elicits the development of inflammatory lung lesions that continue to expand throughout infection. This lesion development and persistence are poorly understood. Here, we examine spatially distinct regions of lung lesions using laser capture microdissection and transcriptome sequencing (RNA-seq) analysis to identify transcriptional differences between lesion microenvironments. We show that cellular pathways involved in leukocyte migration and apoptosis are downregulated in the center of lung lesions compared to the periphery. Probing for the bacterial factor(s) important for the alteration in neutrophil survival, we show both in vitro and in vivo that Y. pestis increases neutrophil survival in a manner that is dependent on the type III secretion system effector YopM. This research explores the complexity of spatially distinct host-microbe interactions and emphasizes the importance of cell relevance in assays in order to fully understand Y. pestis virulence. American Society of Microbiology 2015-10-13 /pmc/articles/PMC4620470/ /pubmed/26463167 http://dx.doi.org/10.1128/mBio.01530-15 Text en Copyright © 2015 Stasulli et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Stasulli, Nikolas M.
Eichelberger, Kara R.
Price, Paul A.
Pechous, Roger D.
Montgomery, Stephanie A.
Parker, Joel S.
Goldman, William E.
Spatially Distinct Neutrophil Responses within the Inflammatory Lesions of Pneumonic Plague
title Spatially Distinct Neutrophil Responses within the Inflammatory Lesions of Pneumonic Plague
title_full Spatially Distinct Neutrophil Responses within the Inflammatory Lesions of Pneumonic Plague
title_fullStr Spatially Distinct Neutrophil Responses within the Inflammatory Lesions of Pneumonic Plague
title_full_unstemmed Spatially Distinct Neutrophil Responses within the Inflammatory Lesions of Pneumonic Plague
title_short Spatially Distinct Neutrophil Responses within the Inflammatory Lesions of Pneumonic Plague
title_sort spatially distinct neutrophil responses within the inflammatory lesions of pneumonic plague
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4620470/
https://www.ncbi.nlm.nih.gov/pubmed/26463167
http://dx.doi.org/10.1128/mBio.01530-15
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