High-throughput genotyping of CRISPR/Cas9-mediated mutants using fluorescent PCR-capillary gel electrophoresis

Recent advances in the engineering of sequence-specific synthetic nucleases provide enormous opportunities for genetic manipulation of gene expression in order to study their cellular function in vivo. However, current genotyping methods to detect these programmable nuclease-induced insertion/deleti...

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Detalles Bibliográficos
Autores principales: Ramlee, Muhammad Khairul, Yan, Tingdong, Cheung, Alice M. S., Chuah, Charles T. H., Li, Shang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4620477/
https://www.ncbi.nlm.nih.gov/pubmed/26498861
http://dx.doi.org/10.1038/srep15587

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4620477/
https://www.ncbi.nlm.nih.gov/pubmed/26498861
http://dx.doi.org/10.1038/srep15587

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