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High-throughput genotyping of CRISPR/Cas9-mediated mutants using fluorescent PCR-capillary gel electrophoresis

Recent advances in the engineering of sequence-specific synthetic nucleases provide enormous opportunities for genetic manipulation of gene expression in order to study their cellular function in vivo. However, current genotyping methods to detect these programmable nuclease-induced insertion/deleti...

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Detalles Bibliográficos
Autores principales:	Ramlee, Muhammad Khairul, Yan, Tingdong, Cheung, Alice M. S., Chuah, Charles T. H., Li, Shang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2015
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4620477/ https://www.ncbi.nlm.nih.gov/pubmed/26498861 http://dx.doi.org/10.1038/srep15587

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