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Enhanced osteogenic potential of mesenchymal stem cells from cortical bone: a comparative analysis

INTRODUCTION: Mesenchymal stem cells (MSCs) hold great promise for regenerative therapies in the musculoskeletal system. Although MSCs from bone marrow (BM-MSCs) and adipose tissue (AD-MSCs) have been extensively characterized, there is still debate as to the ideal source of MSCs for tissue-engineer...

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Autores principales: Fernandez-Moure, Joseph S., Corradetti, Bruna, Chan, Paige, Van Eps, Jeffrey L., Janecek, Trevor, Rameshwar, Pranela, Weiner, Bradley K., Tasciotti, Ennio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4620594/
https://www.ncbi.nlm.nih.gov/pubmed/26503337
http://dx.doi.org/10.1186/s13287-015-0193-z
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author Fernandez-Moure, Joseph S.
Corradetti, Bruna
Chan, Paige
Van Eps, Jeffrey L.
Janecek, Trevor
Rameshwar, Pranela
Weiner, Bradley K.
Tasciotti, Ennio
author_facet Fernandez-Moure, Joseph S.
Corradetti, Bruna
Chan, Paige
Van Eps, Jeffrey L.
Janecek, Trevor
Rameshwar, Pranela
Weiner, Bradley K.
Tasciotti, Ennio
author_sort Fernandez-Moure, Joseph S.
collection PubMed
description INTRODUCTION: Mesenchymal stem cells (MSCs) hold great promise for regenerative therapies in the musculoskeletal system. Although MSCs from bone marrow (BM-MSCs) and adipose tissue (AD-MSCs) have been extensively characterized, there is still debate as to the ideal source of MSCs for tissue-engineering applications in bone repair. METHODS: MSCs were isolated from cortical bone fragments (CBF-MSCs) obtained from patients undergoing laminectomy, selected by fluorescence-activated cell sorting analysis, and tested for their potential to undergo mesodermic differentiation. CBF-MSCs were then compared with BM-MSCs and AD-MSCs for their colony-forming unit capability and osteogenic potential in both normoxia and hypoxia. After 2 and 4 weeks in inducing media, differentiation was assessed qualitatively and quantitatively by the evaluation of alkaline phosphatase (ALP) expression and mineral deposition (Von Kossa staining). Transcriptional activity of osteoblastogenesis-associated genes (Alp, RUNX2, Spp1, and Bglap) was also analyzed. RESULTS: The cortical fraction of the bone contains a subset of cells positive for MSC-associated markers and capable of tri-lineage differentiation. The hypoxic conditions were generally more effective in inducing osteogenesis for the three cell lines. However, at 2 and 4 weeks, greater calcium deposition and ALP expression were observed in both hypoxic and normoxic conditions in CBF-MSCs compared with AD- and BM-MSCs. These functional observations were further corroborated by gene expression analysis, which showed a significant upregulation of Bglap, Alp, and Spp1, with a 22.50 (±4.55)-, 46.56 (±7.4)-, 71.46 (±4.16)-fold increase compared with their uninduced counterparts. CONCLUSIONS: This novel population of MSCs retains a greater biosynthetic activity in vitro, which was found increased in hypoxic conditions. The present study demonstrates that quantitative differences between MSCs retrieved from bone marrow, adipose, and the cortical portion of the bone with respect to their osteogenic potential exist and suggests the cortical bone as suitable candidate to use for orthopedic tissue engineering and regenerative medicine.
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spelling pubmed-46205942015-10-27 Enhanced osteogenic potential of mesenchymal stem cells from cortical bone: a comparative analysis Fernandez-Moure, Joseph S. Corradetti, Bruna Chan, Paige Van Eps, Jeffrey L. Janecek, Trevor Rameshwar, Pranela Weiner, Bradley K. Tasciotti, Ennio Stem Cell Res Ther Research INTRODUCTION: Mesenchymal stem cells (MSCs) hold great promise for regenerative therapies in the musculoskeletal system. Although MSCs from bone marrow (BM-MSCs) and adipose tissue (AD-MSCs) have been extensively characterized, there is still debate as to the ideal source of MSCs for tissue-engineering applications in bone repair. METHODS: MSCs were isolated from cortical bone fragments (CBF-MSCs) obtained from patients undergoing laminectomy, selected by fluorescence-activated cell sorting analysis, and tested for their potential to undergo mesodermic differentiation. CBF-MSCs were then compared with BM-MSCs and AD-MSCs for their colony-forming unit capability and osteogenic potential in both normoxia and hypoxia. After 2 and 4 weeks in inducing media, differentiation was assessed qualitatively and quantitatively by the evaluation of alkaline phosphatase (ALP) expression and mineral deposition (Von Kossa staining). Transcriptional activity of osteoblastogenesis-associated genes (Alp, RUNX2, Spp1, and Bglap) was also analyzed. RESULTS: The cortical fraction of the bone contains a subset of cells positive for MSC-associated markers and capable of tri-lineage differentiation. The hypoxic conditions were generally more effective in inducing osteogenesis for the three cell lines. However, at 2 and 4 weeks, greater calcium deposition and ALP expression were observed in both hypoxic and normoxic conditions in CBF-MSCs compared with AD- and BM-MSCs. These functional observations were further corroborated by gene expression analysis, which showed a significant upregulation of Bglap, Alp, and Spp1, with a 22.50 (±4.55)-, 46.56 (±7.4)-, 71.46 (±4.16)-fold increase compared with their uninduced counterparts. CONCLUSIONS: This novel population of MSCs retains a greater biosynthetic activity in vitro, which was found increased in hypoxic conditions. The present study demonstrates that quantitative differences between MSCs retrieved from bone marrow, adipose, and the cortical portion of the bone with respect to their osteogenic potential exist and suggests the cortical bone as suitable candidate to use for orthopedic tissue engineering and regenerative medicine. BioMed Central 2015-10-26 /pmc/articles/PMC4620594/ /pubmed/26503337 http://dx.doi.org/10.1186/s13287-015-0193-z Text en © Fernandez-Moure et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Fernandez-Moure, Joseph S.
Corradetti, Bruna
Chan, Paige
Van Eps, Jeffrey L.
Janecek, Trevor
Rameshwar, Pranela
Weiner, Bradley K.
Tasciotti, Ennio
Enhanced osteogenic potential of mesenchymal stem cells from cortical bone: a comparative analysis
title Enhanced osteogenic potential of mesenchymal stem cells from cortical bone: a comparative analysis
title_full Enhanced osteogenic potential of mesenchymal stem cells from cortical bone: a comparative analysis
title_fullStr Enhanced osteogenic potential of mesenchymal stem cells from cortical bone: a comparative analysis
title_full_unstemmed Enhanced osteogenic potential of mesenchymal stem cells from cortical bone: a comparative analysis
title_short Enhanced osteogenic potential of mesenchymal stem cells from cortical bone: a comparative analysis
title_sort enhanced osteogenic potential of mesenchymal stem cells from cortical bone: a comparative analysis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4620594/
https://www.ncbi.nlm.nih.gov/pubmed/26503337
http://dx.doi.org/10.1186/s13287-015-0193-z
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