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Hyperspectral Imaging as an Early Biomarker for Radiation Exposure and Microcirculatory Damage
BACKGROUND: Radiation exposure can lead to detrimental effects in skin microcirculation. The precise relationship between radiation dose received and its effect on cutaneous perfusion still remains controversial. Previously, we have shown that hyperspectral imaging (HSI) is able to demonstrate long-...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4620692/ https://www.ncbi.nlm.nih.gov/pubmed/26579490 http://dx.doi.org/10.3389/fonc.2015.00232 |
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author | Chin, Michael S. Freniere, Brian B. Lancerotto, Luca Lujan-Hernandez, Jorge Saleeby, Jonathan H. Lo, Yuan-Chyuan Orgill, Dennis P. Lalikos, Janice F. Fitzgerald, Thomas J. |
author_facet | Chin, Michael S. Freniere, Brian B. Lancerotto, Luca Lujan-Hernandez, Jorge Saleeby, Jonathan H. Lo, Yuan-Chyuan Orgill, Dennis P. Lalikos, Janice F. Fitzgerald, Thomas J. |
author_sort | Chin, Michael S. |
collection | PubMed |
description | BACKGROUND: Radiation exposure can lead to detrimental effects in skin microcirculation. The precise relationship between radiation dose received and its effect on cutaneous perfusion still remains controversial. Previously, we have shown that hyperspectral imaging (HSI) is able to demonstrate long-term reductions in cutaneous perfusion secondary to chronic microvascular injury. This study characterizes the changes in skin microcirculation in response to varying doses of ionizing radiation and investigates these microcirculatory changes as a possible early non-invasive biomarker that may correlate with the extent of long-term microvascular damage. METHODS: Immunocompetent hairless mice (n = 66) were exposed to single fractions of superficial beta-irradiation in doses of 0, 5, 10, 20, 35, or 50 Gy. A HSI device was utilized to measure deoxygenated hemoglobin levels in irradiated and control areas. HSI measurements were performed at baseline before radiation exposure and for the first 3 days post-irradiation. Maximum macroscopic skin reactions were graded, and histological assessment of cutaneous microvascular densities at 4 weeks post-irradiation was performed in harvested tissue by CD31 immunohistochemistry. RESULTS: CD31 immunohistochemistry demonstrated a significant correlation (r = 0.90, p < 0.0001) between dose and vessel density reduction at 4 weeks. Using HSI analysis, early changes in deoxygenated hemoglobin levels were observed during the first 3 days post-irradiation in all groups. These deoxygenated hemoglobin changes varied proportionally with dose (r = 0.98, p < 0.0001) and skin reactions (r = 0.98, p < 0.0001). There was a highly significant correlation (r = 0.91, p < 0.0001) between these early changes in deoxygenated hemoglobin and late vascular injury severity assessed at the end of 4 weeks. CONCLUSION: Radiation dose is directly correlated with cutaneous microvascular injury severity at 4 weeks in our model. Early post-exposure measurement of cutaneous deoxygenated hemoglobin levels may be a useful biomarker for radiation dose reconstruction and predictor for chronic microvascular injury. |
format | Online Article Text |
id | pubmed-4620692 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-46206922015-11-17 Hyperspectral Imaging as an Early Biomarker for Radiation Exposure and Microcirculatory Damage Chin, Michael S. Freniere, Brian B. Lancerotto, Luca Lujan-Hernandez, Jorge Saleeby, Jonathan H. Lo, Yuan-Chyuan Orgill, Dennis P. Lalikos, Janice F. Fitzgerald, Thomas J. Front Oncol Oncology BACKGROUND: Radiation exposure can lead to detrimental effects in skin microcirculation. The precise relationship between radiation dose received and its effect on cutaneous perfusion still remains controversial. Previously, we have shown that hyperspectral imaging (HSI) is able to demonstrate long-term reductions in cutaneous perfusion secondary to chronic microvascular injury. This study characterizes the changes in skin microcirculation in response to varying doses of ionizing radiation and investigates these microcirculatory changes as a possible early non-invasive biomarker that may correlate with the extent of long-term microvascular damage. METHODS: Immunocompetent hairless mice (n = 66) were exposed to single fractions of superficial beta-irradiation in doses of 0, 5, 10, 20, 35, or 50 Gy. A HSI device was utilized to measure deoxygenated hemoglobin levels in irradiated and control areas. HSI measurements were performed at baseline before radiation exposure and for the first 3 days post-irradiation. Maximum macroscopic skin reactions were graded, and histological assessment of cutaneous microvascular densities at 4 weeks post-irradiation was performed in harvested tissue by CD31 immunohistochemistry. RESULTS: CD31 immunohistochemistry demonstrated a significant correlation (r = 0.90, p < 0.0001) between dose and vessel density reduction at 4 weeks. Using HSI analysis, early changes in deoxygenated hemoglobin levels were observed during the first 3 days post-irradiation in all groups. These deoxygenated hemoglobin changes varied proportionally with dose (r = 0.98, p < 0.0001) and skin reactions (r = 0.98, p < 0.0001). There was a highly significant correlation (r = 0.91, p < 0.0001) between these early changes in deoxygenated hemoglobin and late vascular injury severity assessed at the end of 4 weeks. CONCLUSION: Radiation dose is directly correlated with cutaneous microvascular injury severity at 4 weeks in our model. Early post-exposure measurement of cutaneous deoxygenated hemoglobin levels may be a useful biomarker for radiation dose reconstruction and predictor for chronic microvascular injury. Frontiers Media S.A. 2015-10-26 /pmc/articles/PMC4620692/ /pubmed/26579490 http://dx.doi.org/10.3389/fonc.2015.00232 Text en Copyright © 2015 Chin, Freniere, Lancerotto, Lujan-Hernandez, Saleeby, Lo, Orgill, Lalikos and Fitzgerald. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Chin, Michael S. Freniere, Brian B. Lancerotto, Luca Lujan-Hernandez, Jorge Saleeby, Jonathan H. Lo, Yuan-Chyuan Orgill, Dennis P. Lalikos, Janice F. Fitzgerald, Thomas J. Hyperspectral Imaging as an Early Biomarker for Radiation Exposure and Microcirculatory Damage |
title | Hyperspectral Imaging as an Early Biomarker for Radiation Exposure and Microcirculatory Damage |
title_full | Hyperspectral Imaging as an Early Biomarker for Radiation Exposure and Microcirculatory Damage |
title_fullStr | Hyperspectral Imaging as an Early Biomarker for Radiation Exposure and Microcirculatory Damage |
title_full_unstemmed | Hyperspectral Imaging as an Early Biomarker for Radiation Exposure and Microcirculatory Damage |
title_short | Hyperspectral Imaging as an Early Biomarker for Radiation Exposure and Microcirculatory Damage |
title_sort | hyperspectral imaging as an early biomarker for radiation exposure and microcirculatory damage |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4620692/ https://www.ncbi.nlm.nih.gov/pubmed/26579490 http://dx.doi.org/10.3389/fonc.2015.00232 |
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