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A new method of wound treatment: targeted therapy of skin wounds with reactive oxygen species-responsive nanoparticles containing SDF-1α

OBJECTIVE: To accelerate wound healing through promoting vascularization by using reactive oxygen species (ROS)-responsive nanoparticles loaded with stromal cell-derived factor-1α(SDF-1α). METHODS: The ROS-reactive nanomaterial poly-(1,4-phenyleneacetone dimethylene thioketal) was synthesized, and i...

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Autores principales: Tang, Tao, Jiang, Hao, Yu, Yuan, He, Fang, Ji, Shi-zhao, Liu, Ying-ying, Wang, Zhong-shan, Xiao, Shi-chu, Tang, Cui, Wang, Guang-Yi, Xia, Zhao-Fan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621221/
https://www.ncbi.nlm.nih.gov/pubmed/26527874
http://dx.doi.org/10.2147/IJN.S88384
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author Tang, Tao
Jiang, Hao
Yu, Yuan
He, Fang
Ji, Shi-zhao
Liu, Ying-ying
Wang, Zhong-shan
Xiao, Shi-chu
Tang, Cui
Wang, Guang-Yi
Xia, Zhao-Fan
author_facet Tang, Tao
Jiang, Hao
Yu, Yuan
He, Fang
Ji, Shi-zhao
Liu, Ying-ying
Wang, Zhong-shan
Xiao, Shi-chu
Tang, Cui
Wang, Guang-Yi
Xia, Zhao-Fan
author_sort Tang, Tao
collection PubMed
description OBJECTIVE: To accelerate wound healing through promoting vascularization by using reactive oxygen species (ROS)-responsive nanoparticles loaded with stromal cell-derived factor-1α(SDF-1α). METHODS: The ROS-reactive nanomaterial poly-(1,4-phenyleneacetone dimethylene thioketal) was synthesized, and its physical and chemical properties were characterized. ROS-responsive nanoparticles containing SDF-1α were prepared through a multiple emulsion solvent evaporation method. The loading capacity, stability, activity of the encapsulated protein, toxicity, and in vivo distribution of these nanoparticles were determined. These nanoparticles were administered by intravenous infusion to mice with full-thickness skin defects to study their effects on the directed chemotaxis of bone marrow mesenchymal stem cells, wound vascularization, and wound healing. RESULTS: The synthesized ROS-reactive organic polymer poly-(1,4-phenyleneacetone dimethylene thioketal) possessed a molecular weight of approximately 11.5 kDa with a dispersity of 1.97. ROS-responsive nanoparticles containing SDF-1α were prepared with an average diameter of 110 nm and a drug loading capacity of 1.8%. The encapsulation process showed minimal effects on the activity of SDF-1α, and it could be effectively released from the nanoparticles in the presence of ROS. Encapsulated SDF-1α could exist for a long time in blood. In mice with full-thickness skin defects, SDF-1α was effectively released and targeted to the wounds, thus promoting the chemotaxis of bone marrow mesenchymal stem cells toward the wound and its periphery, inducing wound vascularization, and accelerating wound healing.
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spelling pubmed-46212212015-11-02 A new method of wound treatment: targeted therapy of skin wounds with reactive oxygen species-responsive nanoparticles containing SDF-1α Tang, Tao Jiang, Hao Yu, Yuan He, Fang Ji, Shi-zhao Liu, Ying-ying Wang, Zhong-shan Xiao, Shi-chu Tang, Cui Wang, Guang-Yi Xia, Zhao-Fan Int J Nanomedicine Original Research OBJECTIVE: To accelerate wound healing through promoting vascularization by using reactive oxygen species (ROS)-responsive nanoparticles loaded with stromal cell-derived factor-1α(SDF-1α). METHODS: The ROS-reactive nanomaterial poly-(1,4-phenyleneacetone dimethylene thioketal) was synthesized, and its physical and chemical properties were characterized. ROS-responsive nanoparticles containing SDF-1α were prepared through a multiple emulsion solvent evaporation method. The loading capacity, stability, activity of the encapsulated protein, toxicity, and in vivo distribution of these nanoparticles were determined. These nanoparticles were administered by intravenous infusion to mice with full-thickness skin defects to study their effects on the directed chemotaxis of bone marrow mesenchymal stem cells, wound vascularization, and wound healing. RESULTS: The synthesized ROS-reactive organic polymer poly-(1,4-phenyleneacetone dimethylene thioketal) possessed a molecular weight of approximately 11.5 kDa with a dispersity of 1.97. ROS-responsive nanoparticles containing SDF-1α were prepared with an average diameter of 110 nm and a drug loading capacity of 1.8%. The encapsulation process showed minimal effects on the activity of SDF-1α, and it could be effectively released from the nanoparticles in the presence of ROS. Encapsulated SDF-1α could exist for a long time in blood. In mice with full-thickness skin defects, SDF-1α was effectively released and targeted to the wounds, thus promoting the chemotaxis of bone marrow mesenchymal stem cells toward the wound and its periphery, inducing wound vascularization, and accelerating wound healing. Dove Medical Press 2015-10-19 /pmc/articles/PMC4621221/ /pubmed/26527874 http://dx.doi.org/10.2147/IJN.S88384 Text en © 2015 Tang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Tang, Tao
Jiang, Hao
Yu, Yuan
He, Fang
Ji, Shi-zhao
Liu, Ying-ying
Wang, Zhong-shan
Xiao, Shi-chu
Tang, Cui
Wang, Guang-Yi
Xia, Zhao-Fan
A new method of wound treatment: targeted therapy of skin wounds with reactive oxygen species-responsive nanoparticles containing SDF-1α
title A new method of wound treatment: targeted therapy of skin wounds with reactive oxygen species-responsive nanoparticles containing SDF-1α
title_full A new method of wound treatment: targeted therapy of skin wounds with reactive oxygen species-responsive nanoparticles containing SDF-1α
title_fullStr A new method of wound treatment: targeted therapy of skin wounds with reactive oxygen species-responsive nanoparticles containing SDF-1α
title_full_unstemmed A new method of wound treatment: targeted therapy of skin wounds with reactive oxygen species-responsive nanoparticles containing SDF-1α
title_short A new method of wound treatment: targeted therapy of skin wounds with reactive oxygen species-responsive nanoparticles containing SDF-1α
title_sort new method of wound treatment: targeted therapy of skin wounds with reactive oxygen species-responsive nanoparticles containing sdf-1α
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621221/
https://www.ncbi.nlm.nih.gov/pubmed/26527874
http://dx.doi.org/10.2147/IJN.S88384
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