Interpretation of Negative Molecular Test Results in Patients With Suspected or Confirmed Ebola Virus Disease: Report of Two Cases

Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) is the most sensitive quantitative diagnostic assay for detection of Ebola virus in multiple body fluids. Despite the strengths of this assay, we present 2 cases of Ebola virus disease (EVD) and highlight the potential for false-...

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Autores principales: Edwards, Jeffrey K., Kleine, Christian, Munster, Vincent, Giuliani, Ruggero, Massaquoi, Moses, Sprecher, Armand, Chertow, Daniel S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621457/
https://www.ncbi.nlm.nih.gov/pubmed/26512358
http://dx.doi.org/10.1093/ofid/ofv137
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author Edwards, Jeffrey K.
Kleine, Christian
Munster, Vincent
Giuliani, Ruggero
Massaquoi, Moses
Sprecher, Armand
Chertow, Daniel S.
author_facet Edwards, Jeffrey K.
Kleine, Christian
Munster, Vincent
Giuliani, Ruggero
Massaquoi, Moses
Sprecher, Armand
Chertow, Daniel S.
author_sort Edwards, Jeffrey K.
collection PubMed
description Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) is the most sensitive quantitative diagnostic assay for detection of Ebola virus in multiple body fluids. Despite the strengths of this assay, we present 2 cases of Ebola virus disease (EVD) and highlight the potential for false-negative results during the early and late stages of EVD. The first case emphasizes the low negative-predictive value of qRT-PCR during incubation and the early febrile stage of EVD, and the second case emphasizes the potential for false-negative results during recovery and late neurologic complications of EVD. Careful interpretation of test results are needed to guide difficult admission and discharge decisions in suspected or confirmed EVD.
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spelling pubmed-46214572015-10-28 Interpretation of Negative Molecular Test Results in Patients With Suspected or Confirmed Ebola Virus Disease: Report of Two Cases Edwards, Jeffrey K. Kleine, Christian Munster, Vincent Giuliani, Ruggero Massaquoi, Moses Sprecher, Armand Chertow, Daniel S. Open Forum Infect Dis Brief Reports Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) is the most sensitive quantitative diagnostic assay for detection of Ebola virus in multiple body fluids. Despite the strengths of this assay, we present 2 cases of Ebola virus disease (EVD) and highlight the potential for false-negative results during the early and late stages of EVD. The first case emphasizes the low negative-predictive value of qRT-PCR during incubation and the early febrile stage of EVD, and the second case emphasizes the potential for false-negative results during recovery and late neurologic complications of EVD. Careful interpretation of test results are needed to guide difficult admission and discharge decisions in suspected or confirmed EVD. Oxford University Press 2015-09-19 /pmc/articles/PMC4621457/ /pubmed/26512358 http://dx.doi.org/10.1093/ofid/ofv137 Text en © The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.
spellingShingle Brief Reports
Edwards, Jeffrey K.
Kleine, Christian
Munster, Vincent
Giuliani, Ruggero
Massaquoi, Moses
Sprecher, Armand
Chertow, Daniel S.
Interpretation of Negative Molecular Test Results in Patients With Suspected or Confirmed Ebola Virus Disease: Report of Two Cases
title Interpretation of Negative Molecular Test Results in Patients With Suspected or Confirmed Ebola Virus Disease: Report of Two Cases
title_full Interpretation of Negative Molecular Test Results in Patients With Suspected or Confirmed Ebola Virus Disease: Report of Two Cases
title_fullStr Interpretation of Negative Molecular Test Results in Patients With Suspected or Confirmed Ebola Virus Disease: Report of Two Cases
title_full_unstemmed Interpretation of Negative Molecular Test Results in Patients With Suspected or Confirmed Ebola Virus Disease: Report of Two Cases
title_short Interpretation of Negative Molecular Test Results in Patients With Suspected or Confirmed Ebola Virus Disease: Report of Two Cases
title_sort interpretation of negative molecular test results in patients with suspected or confirmed ebola virus disease: report of two cases
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621457/
https://www.ncbi.nlm.nih.gov/pubmed/26512358
http://dx.doi.org/10.1093/ofid/ofv137
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