Affinity Chromatography of Native and Recombinant Proteins from Receptors for Insulin and IGF-I to Recombinant Single Chain Antibodies

Affinity chromatography is an efficient method to isolate proteins by taking advantage of their affinities for specific molecules such as substrates, inhibitors, antigens, ligands, antibodies, and other interacting molecules, including subunits. Nowadays, we take the effectiveness and excellence of...

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Detalles Bibliográficos
Autor principal: Fujita-Yamaguchi, Yoko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Endocrinology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621480/
https://www.ncbi.nlm.nih.gov/pubmed/26579073
http://dx.doi.org/10.3389/fendo.2015.00166
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author Fujita-Yamaguchi, Yoko
author_facet Fujita-Yamaguchi, Yoko
author_sort Fujita-Yamaguchi, Yoko
collection PubMed
description Affinity chromatography is an efficient method to isolate proteins by taking advantage of their affinities for specific molecules such as substrates, inhibitors, antigens, ligands, antibodies, and other interacting molecules, including subunits. Nowadays, we take the effectiveness and excellence of this technology for granted. This essay will mainly cover the use of affinity chromatography based on my experience.
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spelling pubmed-46214802015-11-17 Affinity Chromatography of Native and Recombinant Proteins from Receptors for Insulin and IGF-I to Recombinant Single Chain Antibodies Fujita-Yamaguchi, Yoko Front Endocrinol (Lausanne) Endocrinology Affinity chromatography is an efficient method to isolate proteins by taking advantage of their affinities for specific molecules such as substrates, inhibitors, antigens, ligands, antibodies, and other interacting molecules, including subunits. Nowadays, we take the effectiveness and excellence of this technology for granted. This essay will mainly cover the use of affinity chromatography based on my experience. Frontiers Media S.A. 2015-10-27 /pmc/articles/PMC4621480/ /pubmed/26579073 http://dx.doi.org/10.3389/fendo.2015.00166 Text en Copyright © 2015 Fujita-Yamaguchi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Fujita-Yamaguchi, Yoko
Affinity Chromatography of Native and Recombinant Proteins from Receptors for Insulin and IGF-I to Recombinant Single Chain Antibodies
title Affinity Chromatography of Native and Recombinant Proteins from Receptors for Insulin and IGF-I to Recombinant Single Chain Antibodies
title_full Affinity Chromatography of Native and Recombinant Proteins from Receptors for Insulin and IGF-I to Recombinant Single Chain Antibodies
title_fullStr Affinity Chromatography of Native and Recombinant Proteins from Receptors for Insulin and IGF-I to Recombinant Single Chain Antibodies
title_full_unstemmed Affinity Chromatography of Native and Recombinant Proteins from Receptors for Insulin and IGF-I to Recombinant Single Chain Antibodies
title_short Affinity Chromatography of Native and Recombinant Proteins from Receptors for Insulin and IGF-I to Recombinant Single Chain Antibodies
title_sort affinity chromatography of native and recombinant proteins from receptors for insulin and igf-i to recombinant single chain antibodies
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621480/
https://www.ncbi.nlm.nih.gov/pubmed/26579073
http://dx.doi.org/10.3389/fendo.2015.00166
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