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Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly

During meiosis, the kinetochore undergoes substantial reorganization to establish monopolar spindle attachment. In the fission yeast Schizosaccharomyces pombe, the KNL1–Spc7-Mis12-Nuf2 (KMN) complex, which constitutes the outer kinetochore, is disassembled during meiotic prophase and is reassembled...

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Autores principales: Yang, Hui-Ju, Asakawa, Haruhiko, Haraguchi, Tokuko, Hiraoka, Yasushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621824/
https://www.ncbi.nlm.nih.gov/pubmed/26483559
http://dx.doi.org/10.1083/jcb.201501035
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author Yang, Hui-Ju
Asakawa, Haruhiko
Haraguchi, Tokuko
Hiraoka, Yasushi
author_facet Yang, Hui-Ju
Asakawa, Haruhiko
Haraguchi, Tokuko
Hiraoka, Yasushi
author_sort Yang, Hui-Ju
collection PubMed
description During meiosis, the kinetochore undergoes substantial reorganization to establish monopolar spindle attachment. In the fission yeast Schizosaccharomyces pombe, the KNL1–Spc7-Mis12-Nuf2 (KMN) complex, which constitutes the outer kinetochore, is disassembled during meiotic prophase and is reassembled before meiosis I. Here, we show that the nucleoporin Nup132 is required for timely assembly of the KMN proteins: In the absence of Nup132, Mis12 and Spc7 are precociously assembled at the centromeres during meiotic prophase. In contrast, Nuf2 shows timely dissociation and reappearance at the meiotic centromeres. We further demonstrate that depletion of Nup132 activates the spindle assembly checkpoint in meiosis I, possibly because of the increased incidence of erroneous spindle attachment at sister chromatids. These results suggest that precocious assembly of the kinetochores leads to the meiosis I defects observed in the nup132-disrupted mutant. Thus, we propose that Nup132 plays an important role in establishing monopolar spindle attachment at meiosis I through outer kinetochore reorganization at meiotic prophase.
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spelling pubmed-46218242016-04-26 Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly Yang, Hui-Ju Asakawa, Haruhiko Haraguchi, Tokuko Hiraoka, Yasushi J Cell Biol Research Articles During meiosis, the kinetochore undergoes substantial reorganization to establish monopolar spindle attachment. In the fission yeast Schizosaccharomyces pombe, the KNL1–Spc7-Mis12-Nuf2 (KMN) complex, which constitutes the outer kinetochore, is disassembled during meiotic prophase and is reassembled before meiosis I. Here, we show that the nucleoporin Nup132 is required for timely assembly of the KMN proteins: In the absence of Nup132, Mis12 and Spc7 are precociously assembled at the centromeres during meiotic prophase. In contrast, Nuf2 shows timely dissociation and reappearance at the meiotic centromeres. We further demonstrate that depletion of Nup132 activates the spindle assembly checkpoint in meiosis I, possibly because of the increased incidence of erroneous spindle attachment at sister chromatids. These results suggest that precocious assembly of the kinetochores leads to the meiosis I defects observed in the nup132-disrupted mutant. Thus, we propose that Nup132 plays an important role in establishing monopolar spindle attachment at meiosis I through outer kinetochore reorganization at meiotic prophase. The Rockefeller University Press 2015-10-26 /pmc/articles/PMC4621824/ /pubmed/26483559 http://dx.doi.org/10.1083/jcb.201501035 Text en © 2015 Yang et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Yang, Hui-Ju
Asakawa, Haruhiko
Haraguchi, Tokuko
Hiraoka, Yasushi
Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly
title Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly
title_full Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly
title_fullStr Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly
title_full_unstemmed Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly
title_short Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly
title_sort nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621824/
https://www.ncbi.nlm.nih.gov/pubmed/26483559
http://dx.doi.org/10.1083/jcb.201501035
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