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The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135

In polarized epithelial cells, newly synthesized cell surface proteins travel in carrier vesicles from the trans Golgi network to the apical or basolateral plasma membrane. Despite extensive research on polarized trafficking, the sites of protein delivery are not fully characterized. Here we use the...

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Autores principales: Stoops, Emily H., Hull, Michael, Olesen, Christina, Mistry, Kavita, Harder, Jennifer L., Rivera-Molina, Felix, Toomre, Derek, Caplan, Michael J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621837/
https://www.ncbi.nlm.nih.gov/pubmed/26504168
http://dx.doi.org/10.1083/jcb.201502045
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author Stoops, Emily H.
Hull, Michael
Olesen, Christina
Mistry, Kavita
Harder, Jennifer L.
Rivera-Molina, Felix
Toomre, Derek
Caplan, Michael J.
author_facet Stoops, Emily H.
Hull, Michael
Olesen, Christina
Mistry, Kavita
Harder, Jennifer L.
Rivera-Molina, Felix
Toomre, Derek
Caplan, Michael J.
author_sort Stoops, Emily H.
collection PubMed
description In polarized epithelial cells, newly synthesized cell surface proteins travel in carrier vesicles from the trans Golgi network to the apical or basolateral plasma membrane. Despite extensive research on polarized trafficking, the sites of protein delivery are not fully characterized. Here we use the SNAP tag system to examine the site of delivery of the apical glycoprotein gp135. We show that a cohort of gp135 is delivered to a ring surrounding the base of the primary cilium, followed by microtubule-dependent radial movement away from the cilium. Delivery to the periciliary ring was specific to newly synthesized and not recycling protein. A subset of this newly delivered protein traverses the basolateral membrane en route to the apical membrane. Crumbs3a, another apical protein, was not delivered to the periciliary region, instead making its initial apical appearance in a pattern that resembled its steady-state distribution. Our results demonstrate a surprising “hot spot” for gp135 protein delivery at the base of the primary cilium and suggest the existence of a novel microtubule-based directed movement of a subset of apical surface proteins.
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spelling pubmed-46218372016-04-26 The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135 Stoops, Emily H. Hull, Michael Olesen, Christina Mistry, Kavita Harder, Jennifer L. Rivera-Molina, Felix Toomre, Derek Caplan, Michael J. J Cell Biol Research Articles In polarized epithelial cells, newly synthesized cell surface proteins travel in carrier vesicles from the trans Golgi network to the apical or basolateral plasma membrane. Despite extensive research on polarized trafficking, the sites of protein delivery are not fully characterized. Here we use the SNAP tag system to examine the site of delivery of the apical glycoprotein gp135. We show that a cohort of gp135 is delivered to a ring surrounding the base of the primary cilium, followed by microtubule-dependent radial movement away from the cilium. Delivery to the periciliary ring was specific to newly synthesized and not recycling protein. A subset of this newly delivered protein traverses the basolateral membrane en route to the apical membrane. Crumbs3a, another apical protein, was not delivered to the periciliary region, instead making its initial apical appearance in a pattern that resembled its steady-state distribution. Our results demonstrate a surprising “hot spot” for gp135 protein delivery at the base of the primary cilium and suggest the existence of a novel microtubule-based directed movement of a subset of apical surface proteins. The Rockefeller University Press 2015-10-26 /pmc/articles/PMC4621837/ /pubmed/26504168 http://dx.doi.org/10.1083/jcb.201502045 Text en © 2015 Stoops et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Stoops, Emily H.
Hull, Michael
Olesen, Christina
Mistry, Kavita
Harder, Jennifer L.
Rivera-Molina, Felix
Toomre, Derek
Caplan, Michael J.
The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135
title The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135
title_full The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135
title_fullStr The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135
title_full_unstemmed The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135
title_short The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135
title_sort periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621837/
https://www.ncbi.nlm.nih.gov/pubmed/26504168
http://dx.doi.org/10.1083/jcb.201502045
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