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Liposome encapsulated curcumin-difluorinated (CDF) inhibits the growth of cisplatin resistant head and neck cancer stem cells

Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer, with 600,000 new cases every year worldwide. Although chemotherapeutics exist, five-year survival is only 50%. New strategies to overcome drug resistance are required to improve HNSCC treatment. Curcumin-difluorinated (CD...

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Autores principales: Basak, Saroj K., Zinabadi, Alborz, Wu, Arthur W., Venkatesan, Natarajan, Duarte, Victor M., Kang, James J., Dalgard, Clifton L., Srivastava, Meera, Sarkar, Fazlul H., Wang, Marilene B., Srivatsan, Eri S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621906/
https://www.ncbi.nlm.nih.gov/pubmed/26098778
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author Basak, Saroj K.
Zinabadi, Alborz
Wu, Arthur W.
Venkatesan, Natarajan
Duarte, Victor M.
Kang, James J.
Dalgard, Clifton L.
Srivastava, Meera
Sarkar, Fazlul H.
Wang, Marilene B.
Srivatsan, Eri S.
author_facet Basak, Saroj K.
Zinabadi, Alborz
Wu, Arthur W.
Venkatesan, Natarajan
Duarte, Victor M.
Kang, James J.
Dalgard, Clifton L.
Srivastava, Meera
Sarkar, Fazlul H.
Wang, Marilene B.
Srivatsan, Eri S.
author_sort Basak, Saroj K.
collection PubMed
description Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer, with 600,000 new cases every year worldwide. Although chemotherapeutics exist, five-year survival is only 50%. New strategies to overcome drug resistance are required to improve HNSCC treatment. Curcumin-difluorinated (CDF), a synthetic analog of curcumin, was packaged in liposomes and used to evaluate growth inhibition of cisplatin resistant HNSCC cell lines CCL-23R and UM-SCC-1R generated from the parental cell lines CCL-23 and UM-SCC-1 respectively. Growth inhibition in vitro and expression levels of the CD44 (cancer stem cell marker), cytokines, and growth factors were investigated after liposomal CDF treatment. The in vivo growth inhibitory effect of liposomal CDF was evaluated in the nude mice xenograft tumor model of UM-SCC-1R and the inhibition of CD44 was measured. Treatment of the resistant cell lines in vitro with liposomal CDF resulted in a statistically significant growth inhibition (p < 0.05). The nude mice xenograft study showed a statistically significant tumor growth inhibition of UM-SCC-1R cells and a reduction in the expression of CD44 (p < 0.05), indicating an inhibitory effect of liposomal CDF on CSCs. Our results demonstrate that delivery of CDF through liposomes may be an effective method for the treatment of cisplatin resistant HNSCC.
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spelling pubmed-46219062015-12-02 Liposome encapsulated curcumin-difluorinated (CDF) inhibits the growth of cisplatin resistant head and neck cancer stem cells Basak, Saroj K. Zinabadi, Alborz Wu, Arthur W. Venkatesan, Natarajan Duarte, Victor M. Kang, James J. Dalgard, Clifton L. Srivastava, Meera Sarkar, Fazlul H. Wang, Marilene B. Srivatsan, Eri S. Oncotarget Research Paper Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer, with 600,000 new cases every year worldwide. Although chemotherapeutics exist, five-year survival is only 50%. New strategies to overcome drug resistance are required to improve HNSCC treatment. Curcumin-difluorinated (CDF), a synthetic analog of curcumin, was packaged in liposomes and used to evaluate growth inhibition of cisplatin resistant HNSCC cell lines CCL-23R and UM-SCC-1R generated from the parental cell lines CCL-23 and UM-SCC-1 respectively. Growth inhibition in vitro and expression levels of the CD44 (cancer stem cell marker), cytokines, and growth factors were investigated after liposomal CDF treatment. The in vivo growth inhibitory effect of liposomal CDF was evaluated in the nude mice xenograft tumor model of UM-SCC-1R and the inhibition of CD44 was measured. Treatment of the resistant cell lines in vitro with liposomal CDF resulted in a statistically significant growth inhibition (p < 0.05). The nude mice xenograft study showed a statistically significant tumor growth inhibition of UM-SCC-1R cells and a reduction in the expression of CD44 (p < 0.05), indicating an inhibitory effect of liposomal CDF on CSCs. Our results demonstrate that delivery of CDF through liposomes may be an effective method for the treatment of cisplatin resistant HNSCC. Impact Journals LLC 2015-05-19 /pmc/articles/PMC4621906/ /pubmed/26098778 Text en Copyright: © 2015 Basak et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Basak, Saroj K.
Zinabadi, Alborz
Wu, Arthur W.
Venkatesan, Natarajan
Duarte, Victor M.
Kang, James J.
Dalgard, Clifton L.
Srivastava, Meera
Sarkar, Fazlul H.
Wang, Marilene B.
Srivatsan, Eri S.
Liposome encapsulated curcumin-difluorinated (CDF) inhibits the growth of cisplatin resistant head and neck cancer stem cells
title Liposome encapsulated curcumin-difluorinated (CDF) inhibits the growth of cisplatin resistant head and neck cancer stem cells
title_full Liposome encapsulated curcumin-difluorinated (CDF) inhibits the growth of cisplatin resistant head and neck cancer stem cells
title_fullStr Liposome encapsulated curcumin-difluorinated (CDF) inhibits the growth of cisplatin resistant head and neck cancer stem cells
title_full_unstemmed Liposome encapsulated curcumin-difluorinated (CDF) inhibits the growth of cisplatin resistant head and neck cancer stem cells
title_short Liposome encapsulated curcumin-difluorinated (CDF) inhibits the growth of cisplatin resistant head and neck cancer stem cells
title_sort liposome encapsulated curcumin-difluorinated (cdf) inhibits the growth of cisplatin resistant head and neck cancer stem cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621906/
https://www.ncbi.nlm.nih.gov/pubmed/26098778
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