Cargando…
Analysis of the reaction of subcutaneous tissues in rats and the antimicrobial activity of calcium hydroxide paste used in association with different substances
The aim of this study was to evaluate the subcutaneous tissue response in rats and the antimicrobial activity of intracanal calcium hydroxide dressings mixed with different substances against E. faecalis. Fifty four rats were divided into three experimental groups according to the vehicle in the cal...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Faculdade de Odontologia de Bauru da Universidade de São Paulo
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621947/ https://www.ncbi.nlm.nih.gov/pubmed/26200527 http://dx.doi.org/10.1590/1678-775720140049 |
_version_ | 1782397526680272896 |
---|---|
author | MIDENA, Raquel Zanin GARCIA, Roberto Brandão CAVENAGO, Bruno Cavalini MARCIANO, Marina Angélica MINOTTI, Paloma Gagliardi ORDINOLA-ZAPATA, Ronald WECKWERTH, Paulo Henrique de ANDRADE, Flaviana Bombarda DUARTE, Marco Antonio Hungaro |
author_facet | MIDENA, Raquel Zanin GARCIA, Roberto Brandão CAVENAGO, Bruno Cavalini MARCIANO, Marina Angélica MINOTTI, Paloma Gagliardi ORDINOLA-ZAPATA, Ronald WECKWERTH, Paulo Henrique de ANDRADE, Flaviana Bombarda DUARTE, Marco Antonio Hungaro |
author_sort | MIDENA, Raquel Zanin |
collection | PubMed |
description | The aim of this study was to evaluate the subcutaneous tissue response in rats and the antimicrobial activity of intracanal calcium hydroxide dressings mixed with different substances against E. faecalis. Fifty four rats were divided into three experimental groups according to the vehicle in the calcium hydroxide treatment: 0.4% chlorohexidine in propylene glycol (PG), Casearia sylvestris Sw in PG and calcium hydroxide+PG (control group). The pastes were placed into polyethylene tubes and implanted into the subcutaneous tissue. After 7, 14 and 30 days, the samples were processed and histologically evaluated (hematoxylin and eosin). The tissue surface in contact with the material was analyzed, and the quantitative analysis determined the volume density occupied by the inflammatory infiltrate (giant cells, polymorphonuclear cells and mononuclear cells), fibroblasts, collagen fibers and blood vessels. For the antimicrobial analysis, 20 dentin blocks infected with E. faecalis were treated with calcium hydroxide pastes in different vehicles; 0.4% chlorhexidine in PG, PG, extract from Casearia sylvestris Sw in PG and a positive control (infection and without medication) for 7 days. The efficiency of the pastes was evaluated by the live/dead technique and confocal microscopy. The results showed that 0.4% chlorhexidine induced a higher inflammatory response than the other groups. The Casearia sylvestris Sw extract showed satisfactory results in relation to the intensity of the inflammatory response. In the microbiological test, there were no statistical differences between the evaluated intracanal dressings and the percentage of bacterial viability was between 33 and 42%. The control group showed an 86% viability. Antimicrobial components such as chlorhexidine or Casearia sylvestris Sw did not improve the antimicrobial activity against E. faecalis in comparison to the calcium hydroxide+PG treatment. In addition, the incorporation of chlorhexidine in the calcium hydroxide paste promoted the highest inflammatory response. |
format | Online Article Text |
id | pubmed-4621947 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Faculdade de Odontologia de Bauru da Universidade de São Paulo |
record_format | MEDLINE/PubMed |
spelling | pubmed-46219472015-10-27 Analysis of the reaction of subcutaneous tissues in rats and the antimicrobial activity of calcium hydroxide paste used in association with different substances MIDENA, Raquel Zanin GARCIA, Roberto Brandão CAVENAGO, Bruno Cavalini MARCIANO, Marina Angélica MINOTTI, Paloma Gagliardi ORDINOLA-ZAPATA, Ronald WECKWERTH, Paulo Henrique de ANDRADE, Flaviana Bombarda DUARTE, Marco Antonio Hungaro J Appl Oral Sci Original Article The aim of this study was to evaluate the subcutaneous tissue response in rats and the antimicrobial activity of intracanal calcium hydroxide dressings mixed with different substances against E. faecalis. Fifty four rats were divided into three experimental groups according to the vehicle in the calcium hydroxide treatment: 0.4% chlorohexidine in propylene glycol (PG), Casearia sylvestris Sw in PG and calcium hydroxide+PG (control group). The pastes were placed into polyethylene tubes and implanted into the subcutaneous tissue. After 7, 14 and 30 days, the samples were processed and histologically evaluated (hematoxylin and eosin). The tissue surface in contact with the material was analyzed, and the quantitative analysis determined the volume density occupied by the inflammatory infiltrate (giant cells, polymorphonuclear cells and mononuclear cells), fibroblasts, collagen fibers and blood vessels. For the antimicrobial analysis, 20 dentin blocks infected with E. faecalis were treated with calcium hydroxide pastes in different vehicles; 0.4% chlorhexidine in PG, PG, extract from Casearia sylvestris Sw in PG and a positive control (infection and without medication) for 7 days. The efficiency of the pastes was evaluated by the live/dead technique and confocal microscopy. The results showed that 0.4% chlorhexidine induced a higher inflammatory response than the other groups. The Casearia sylvestris Sw extract showed satisfactory results in relation to the intensity of the inflammatory response. In the microbiological test, there were no statistical differences between the evaluated intracanal dressings and the percentage of bacterial viability was between 33 and 42%. The control group showed an 86% viability. Antimicrobial components such as chlorhexidine or Casearia sylvestris Sw did not improve the antimicrobial activity against E. faecalis in comparison to the calcium hydroxide+PG treatment. In addition, the incorporation of chlorhexidine in the calcium hydroxide paste promoted the highest inflammatory response. Faculdade de Odontologia de Bauru da Universidade de São Paulo 2015 /pmc/articles/PMC4621947/ /pubmed/26200527 http://dx.doi.org/10.1590/1678-775720140049 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article MIDENA, Raquel Zanin GARCIA, Roberto Brandão CAVENAGO, Bruno Cavalini MARCIANO, Marina Angélica MINOTTI, Paloma Gagliardi ORDINOLA-ZAPATA, Ronald WECKWERTH, Paulo Henrique de ANDRADE, Flaviana Bombarda DUARTE, Marco Antonio Hungaro Analysis of the reaction of subcutaneous tissues in rats and the antimicrobial activity of calcium hydroxide paste used in association with different substances |
title | Analysis of the reaction of subcutaneous tissues in rats and the antimicrobial activity of calcium hydroxide paste used in association with different substances |
title_full | Analysis of the reaction of subcutaneous tissues in rats and the antimicrobial activity of calcium hydroxide paste used in association with different substances |
title_fullStr | Analysis of the reaction of subcutaneous tissues in rats and the antimicrobial activity of calcium hydroxide paste used in association with different substances |
title_full_unstemmed | Analysis of the reaction of subcutaneous tissues in rats and the antimicrobial activity of calcium hydroxide paste used in association with different substances |
title_short | Analysis of the reaction of subcutaneous tissues in rats and the antimicrobial activity of calcium hydroxide paste used in association with different substances |
title_sort | analysis of the reaction of subcutaneous tissues in rats and the antimicrobial activity of calcium hydroxide paste used in association with different substances |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621947/ https://www.ncbi.nlm.nih.gov/pubmed/26200527 http://dx.doi.org/10.1590/1678-775720140049 |
work_keys_str_mv | AT midenaraquelzanin analysisofthereactionofsubcutaneoustissuesinratsandtheantimicrobialactivityofcalciumhydroxidepasteusedinassociationwithdifferentsubstances AT garciarobertobrandao analysisofthereactionofsubcutaneoustissuesinratsandtheantimicrobialactivityofcalciumhydroxidepasteusedinassociationwithdifferentsubstances AT cavenagobrunocavalini analysisofthereactionofsubcutaneoustissuesinratsandtheantimicrobialactivityofcalciumhydroxidepasteusedinassociationwithdifferentsubstances AT marcianomarinaangelica analysisofthereactionofsubcutaneoustissuesinratsandtheantimicrobialactivityofcalciumhydroxidepasteusedinassociationwithdifferentsubstances AT minottipalomagagliardi analysisofthereactionofsubcutaneoustissuesinratsandtheantimicrobialactivityofcalciumhydroxidepasteusedinassociationwithdifferentsubstances AT ordinolazapataronald analysisofthereactionofsubcutaneoustissuesinratsandtheantimicrobialactivityofcalciumhydroxidepasteusedinassociationwithdifferentsubstances AT weckwerthpaulohenrique analysisofthereactionofsubcutaneoustissuesinratsandtheantimicrobialactivityofcalciumhydroxidepasteusedinassociationwithdifferentsubstances AT deandradeflavianabombarda analysisofthereactionofsubcutaneoustissuesinratsandtheantimicrobialactivityofcalciumhydroxidepasteusedinassociationwithdifferentsubstances AT duartemarcoantoniohungaro analysisofthereactionofsubcutaneoustissuesinratsandtheantimicrobialactivityofcalciumhydroxidepasteusedinassociationwithdifferentsubstances |